Language：Japanese   Publisher：JAPAN HEALTH MEDICINE ASSOCIATION  

Marine product processing companies often discard processing by-products, such as fish heads, skins, bones, and entrails; however, there is a growing demand for their effective utilization. In the present study, we prepared horse mackerel by-products (HMBP) and HMBP hydrolysates (HMBP-H) from the head, abdomen, mesosoma, and fins of horse mackerel (<i>Trachurus japonicus</i>) using protease. The effects of HMBP and HMBP-H on serum lipid concentrations were then examined <i>in vivo</i>. Four-week-old male C57BL/6J mice were fed a high-fat diet (Control, casein 23 wt%) and the control diet supplemented with HMBP and HMBP-H, respectively. HMBP and HMBP-H diets were formulated to comprise that the protein sourced from HMBP and HMBP-H constituted 3.2 wt% of the diets, respectively. After four weeks, blood and organs were collected. Serum triglyceride contents were lower in the HMBP and HMBP-H groups than in the control group. These decreases were partly attributed to reductions in the mRNA expression levels of liver fatty acid synthase and acetyl-CoA carboxylase, which are rate-limiting enzymes for fatty acid synthesis. Furthermore, serum total cholesterol contents were reduced in the HMBP-H group, but not in the HMBP group. This decrease may be related to the increased mRNA expression level of liver cholesterol 7 alpha-hydroxylase, which is the rate-limiting enzyme for cholesterol catabolism. These results suggest the potential of HMBP-H as a functional food material to attenuate hyperlipidemia.

DOI： 10.20685/kenkouigaku.33.4_508

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I033966299

Language：Japanese   Publisher：Japan Trace Nutrients Research Society  

AIN93G diet without manganese carbonate was used as a simple low-manganese diet, and the extent to which
manganese status is reduced when it is administered to growing rats was examined. Twelve 4-week-old male Wistar
rats were divided into two groups, one of which received the AIN93G diet (manganese concentration, 10 μg/g) and
the other the AIN93G diet without manganese carbonate (manganese concentration, 0.3 μg/g) for 4 weeks. There
was no difference in rat growth between the two groups, and no clinical signs related to manganese deficiency were
observed at all in the low-manganese diet group. Organ manganese concentrations were clearly reduced by the low
manganese diet, with values in the low manganese group being 28.4% of the control group in the liver and 49.3%
of the control group in the kidneys. Arginase activity in the liver was also significantly decreased in the low manganese
group, but the decrease was not as pronounced as in the manganese concentration, as 79.2% of the values
were maintained in the low manganese group compared to the control group. In serum biochemical tests, the
low-manganese diet group showed slightly but significantly higher serum iron concentration and transferrin saturation
than the control group. To induce manganese deficiency with a simple low-manganese diet, it may be necessary to
administer a low-manganese diet immediately after weaning for multiple generations.

DOI： 10.51029/jtnrs.41.0_7

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Other Link： https://ndlsearch.ndl.go.jp/books/R000000004-I033884741