Updated on 2025/06/22

写真a

 
IWAKI,Hiroaki
 
Organization
Faculty of Chemistry, Materials and Bioengineering Professor
Title
Professor
External link

Degree

  • Doctor of engineering ( 2000.3 )

Research Areas

  • Life Science / Applied microbiology

  • Environmental Science/Agriculture Science / Conservation of biological resources

  • Life Science / Biodiversity and systematics

  • Life Science / Ecology and environment

  • Environmental Science/Agriculture Science / Environmental load reduction and remediation

Education

  • Kansai University   Graduate School, Division of Engineering   Biotechnology major

    1997.4 - 2000.3

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    Country: Japan

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  • Kansai University   Graduate School, Division of Engineering   Biotechnology major

    1995.4 - 1997.3

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  • Kansai University   Faculty of Engineering   Department of Biotechnology

    1991.4 - 1995.3

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Research History

  • 関西大学/教授

    2016.4

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  • Associate Professor

    2009.4 - 2016.3

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  • Kansai University

    2006.4 - 2009.3

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  • Kansai University

    2003.4 - 2006.3

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  • National Research Council of Canada/Biotechnology Research Institute

    2000.9 - 2003.3

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Professional Memberships

Committee Memberships

  •   日本生物工学会第70回大会実行委員(総務代表)  

    2017.11 - 2018.9   

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  • 日本生物工学会   日本生物工学会関西支部庶務幹事  

    2017.6 - 2019.5   

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  •   日本生物工学会関西支部委員  

    2015.6 - Present   

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  • 日本生物工学会   日本生物工学会関西支部企画幹事  

    2015.6 - 2017.5   

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  • 日本生物工学会   日本生物工学会関西支部若手企画委員会委員  

    2014.1 - 2019.5   

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Papers

  • Complete genome sequence of Paraburkholderia terrae strain KU-46, a 2,4-dinitrophenol-degrading bacterium Reviewed International journal

    Tomoki Tanaka, Kenji Okano, Hiroaki Iwaki

    Microbiology Resource Announcements   13 ( 6 )   e00282-24   2024.6

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/mra.00282-24

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  • Precise microbiome engineering using natural and synthetic bacteriophages targeting an artificial bacterial consortium Reviewed International journal

    Tomoki Tanaka, Ryoga Sugiyama, Yu Sato, Manami Kawaguchi, Kohsuke Honda, Hiroaki Iwaki, Kenji Okano

    Frontiers in Microbiology   15 ( 1403903 )   2024.5

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    Publishing type:Research paper (scientific journal)   Publisher:Frontiers Media SA  

    In natural microbiomes, microorganisms interact with each other and exhibit diverse functions. Microbiome engineering, which enables bacterial knockdown, is a promising method to elucidate the functions of targeted bacteria in microbiomes. However, few methods to selectively kill target microorganisms in the microbiome without affecting the growth of nontarget microorganisms are available. In this study, we focused on the host-specific lytic ability of virulent phages and validated their potency for precise microbiome engineering. In an artificial microbiome consisting of Escherichia coli, Pseudomonas putida, Bacillus subtilis, and Lactiplantibacillus plantarum, the addition of bacteriophages infecting their respective host strains specifically reduced the number of these bacteria more than 10<sup>2</sup> orders. Remarkably, the reduction in target bacteria did not affect the growth of nontarget bacteria, indicating that bacteriophages were effective tools for precise microbiome engineering. Moreover, a virulent derivative of the λ phage was synthesized from prophage DNA in the genome of λ lysogen by in vivo DNA assembly and phage-rebooting techniques, and E. coli-targeted microbiome engineering was achieved. These results propose a novel approach for precise microbiome engineering using bacteriophages, in which virulent phages are synthesized from prophage DNA in lysogenic strains without isolating phages from environmental samples.

    DOI: 10.3389/fmicb.2024.1403903

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  • Subtractive modification of bacterial consortium using antisense peptide nucleic acids. Reviewed International journal

    Tatsuya Hizume, Yu Sato, Hiroaki Iwaki, Kohsuke Honda, Kenji Okano

    Frontiers in microbiology   14   1321428   2024.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    Microbiome engineering is an emerging research field that aims to design an artificial microbiome and modulate its function. In particular, subtractive modification of the microbiome allows us to create an artificial microbiome without the microorganism of interest and to evaluate its functions and interactions with other constituent bacteria. However, few techniques that can specifically remove only a single species from a large number of microorganisms and can be applied universally to a variety of microorganisms have been developed. Antisense peptide nucleic acid (PNA) is a potent designable antimicrobial agent that can be delivered into microbial cells by conjugating with a cell-penetrating peptide (CPP). Here, we tested the efficacy of the conjugate of CPP and PNA (CPP-PNA) as microbiome modifiers. The addition of CPP-PNA specifically inhibited the growth of Escherichia coli and Pseudomonas putida in an artificial bacterial consortium comprising E. coli, P. putida, Pseudomonas fluorescens, and Lactiplantibacillus plantarum. Moreover, the growth inhibition of P. putida promoted the growth of P. fluorescens and inhibited the growth of L. plantarum. These results indicate that CPP-PNA can be used not only for precise microbiome engineering but also for analyzing the growth relationships among constituent microorganisms in the microbiome.

    DOI: 10.3389/fmicb.2023.1321428

    PubMed

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  • Identification and characterization of a pab gene cluster responsible for the 4-aminobenzoate degradation pathway, including its involvement in the formation of a γ-glutamylated intermediate in Paraburkholderia terrae strain KU-15. Reviewed

    Yaxuan Liu, Kenji Okano, Hiroaki Iwaki

    Journal of bioscience and bioengineering   137 ( 1 )   38 - 46   2024.1

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Paraburkholderia terrae strain KU-15 grows on 2- and 4-nitrobenzoate and 2- and 4-aminobenzoate (ABA) as the sole nitrogen and carbon sources. The genes responsible for the potential degradation of 2- and 4-nitrobenzoate and 2-ABA have been predicted from its genome sequence. In this study, we identified the pab operon in P. terrae strain KU-15. This operon is responsible for the 4-ABA degradation pathway, which involves the formation of a γ-glutamylated intermediate. Reverse transcription-polymerase chain reaction revealed that the pab operon was induced by 4-ABA. Herein, studying the deletion of pabA and pabB1 in strain KU-15 and the examining of Escherichia coli expressing the pab operon revealed the involvement of the operon in 4-ABA degradation. The first step of the degradation pathway is the formation of a γ-glutamylated intermediate, whereby 4-ABA is converted to γ-glutamyl-4-carboxyanilide (γ-GCA). Subsequently, γ-GCA is oxidized to protocatechuate. Overexpression of various genes in E. coli and purification of recombinant proteins permitted the functional characterization of relevant pathway proteins: PabA is a γ-GCA synthetase, PabB1-B3 functions in a multicomponent dioxygenase system responsible for γ-GCA dioxygenation, and PabC is a γ-GCA hydrolase that reverses the formation of γ-GCA by PabA.

    DOI: 10.1016/j.jbiosc.2023.11.002

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  • Cloning of two gene clusters involved in the catabolism of 2,4-dinitrophenol by Paraburkholderia sp. strain KU-46 and characterization of the initial DnpAB enzymes and a two-component monooxygenases DnpC1C2 Reviewed International coauthorship

    Yaxuan Liu, Taisei Yamamoto, Nozomi Kohaya, Kota Yamamoto, Kenji Okano, Takaaki Sumiyoshi, Yoshie Hasegawa, Peter C.K. Lau, Hiroaki Iwaki

    Journal of Bioscience and Bioengineering   136 ( 3 )   223 - 231   2023.9

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.jbiosc.2023.05.013

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  • Complete Genome Sequence of Paraburkholderia terrae Strain KU-15, a 2-Nitrobenzoate-Degrading Bacterium Reviewed International journal

    Yaxuan Liu, Kenji Okano, Hiroaki Iwaki

    Microbiology Resource Announcements   11 ( 7 )   2022.7

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    Paraburkholderia terrae strain KU-15 has been investigated for its ability to degrade 2-nitrobenzoate. Here, we report the complete 10,422,345-bp genome of this microorganism, which consists of six circular replicons containing 9,483 protein-coding sequences. The genome carries genes that are potentially responsible for 2-nitrobenzoate and 4-nitirobenzoate degradation.

    DOI: 10.1128/mra.00373-22

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  • Identification and characterization of a novel class of self-sufficient cytochrome P450 hydroxylase involved in cyclohexanecarboxylate degradation in Paraburkholderia terrae strain KU-64 Reviewed

    Taisei Yamamoto, Yoshie Hasegawa, Hiroaki Iwaki

    Bioscience, Biotechnology, and Biochemistry   86 ( 2 )   199 - 208   2022.1

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press ({OUP})  

    DOI: 10.1093/bbb/zbab199

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  • Bactericidal effect of nanostructures <i>via</i> lytic transglycosylases of <i>Escherichia coli</i> Reviewed

    Soma Mimura, Tomohiro Shimizu, Shoso Shingubara, Hiroaki Iwaki, Takeshi Ito

    RSC Advances   12(3):1645-1652 ( 3 )   1645 - 1652   2022.1

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    Publishing type:Research paper (scientific journal)   Publisher:Royal Society of Chemistry (RSC)  

    The time profiles of active cell ratios depended on the growth phase and the absence of some lytic transglycosylases of E. coli. Significant cell damage was not found on the autolysis inhibition condition.

    DOI: 10.1039/d1ra07623j

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  • Identification and characterization of a chc gene cluster responsible for the aromatization pathway of cyclohexanecarboxylate degradation in Sinomonas cyclohexanicum ATCC 51369 Reviewed International coauthorship

    Taisei Yamamoto, Yoshie Hasegawa, Peter C.K. Lau, Hiroaki Iwaki

    Journal of Bioscience and Bioengineering   132(6):621-629 ( 6 )   621 - 629   2021.12

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.jbiosc.2021.08.013

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  • Cloning, expression, and characterization of Baeyer–Villiger monooxygenases from eukaryotic Exophiala jeanselmei strain KUFI-6N Reviewed

    Taisei Yamamoto, Kento Kobayashi, Yoshie Hasegawa, Hiroaki Iwaki

    Bioscience, Biotechnology, and Biochemistry   85 ( 7 )   1675 - 1685   2021.4

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/bbb/zbab079

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  • A novel piperidine degradation mechanism in a newly isolated piperidine degrader <i>Pseudomonas</i> sp. strain KU43P Reviewed

    Taisei Yamamoto, Yaxuan Liu, Takaaki Sumiyoshi, Yoshie Hasegawa, Hiroaki Iwaki

    The Journal of General and Applied Microbiology   66 ( 5 )   256 - 264   2020.11

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Microbiology Research Foundation  

    DOI: 10.2323/jgam.2019.11.006

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  • Complete Genome Sequence of Mameliella alba Strain KU6B, a Cyclohexylamine-Utilizing Marine Bacterium Reviewed

    Taisei Yamamoto, Yaxuan Liu, Yoshie Hasegawa, Hiroaki Iwaki

    Microbiology Resource Announcements   Volume 9Issue 19e00273-20 ( 19 )   2020.5

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    Here, we report the complete genome sequence of
    <named-content content-type="genus-species">Mameliella alba</named-content>
    strain KU6B, a bacterium newly isolated from seawater of Boso Peninsula in Japan that is capable of utilizing cyclohexylamine. The complete genome contained a 5,386,988-bp circular chromosome and three circular plasmids of 256,516, 112,434, and 76,727 bp.

    DOI: 10.1128/mra.00273-20

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  • Complete Genome Sequence of Pseudomonas sp. Strain KUIN-1, a Model Strain for Studies on the Production of Cell-Free Ice Nucleation Proteins Reviewed

    Taisei Yamamoto, Yoshie Hasegawa, Hidehisa Kawahara, Hiroaki Iwaki

    Microbiology Resource Announcements   Vol.8 Iss.45 e01204-19 p1-3 ( 45 )   2019.11

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    Authorship:Last author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    <italic>Pseudomonas</italic>
    sp. (formerly
    <named-content content-type="genus-species">Pseudomonas fluorescens</named-content>
    ) strain KUIN-1 is an ice-nucleating bacterium that was isolated from the leaves of field beans (
    <named-content content-type="genus-species">Phaseolus vulgaris</named-content>
    L.). This microorganism can release cell-free ice nucleation proteins and shows cold shock-induced freezing tolerance. Here, we report the 6,028,589-bp complete genome sequence of
    <italic>Pseudomonas</italic>
    sp. KUIN-1.

    DOI: 10.1128/MRA.01204-19

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  • Isolation of marine xylene-utilizing bacteria and characterization of Halioxenophilus aromaticivorans gen. nov., sp. nov. and its xylene degradation gene cluster Reviewed

    Hiroaki Iwaki, Taisei Yamamoto, Yoshie Hasegawa

    FEMS Microbiology Letters   365(7), fny042 ( 7 )   2018.4

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    DOI: 10.1093/femsle/fny042

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  • 微生物機能を利用したバイオベースポリマー生産系の構築 Reviewed

    岩木 宏明

    IFO Res. Commun.   31, 134   2017.12

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  • The oxygenating constituent of 3,6-diketocamphane monooxygenase from the CAM plasmid of Pseudomonas putida: the first crystal structure of a type II Baeyer-Villiger monooxygenase Reviewed International coauthorship

    Michail N. Isupov, Ewald Schroeder, Robert P. Gibson, Jean Beecher, Giuliana Donadio, Vahid Saneei, Stephlina A. Dcunha, Emma J. McGhie, Christopher Sayer, Colin F. Davenport, Peter C. Lau, Yoshie Hasegawa, Hiroaki Iwaki, Maria Kadow, Kathleen Balke, Uwe T. Bornscheuer, Gleb Bourenkov, Jennifer A. Littlechild

    ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY   71   2344 - 2353   2015.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1107/S1399004715017939

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  • Human alpha-amino-beta-carboxymuconate-esemialdehyde decarboxylase (ACMSD): A structural and mechanistic unveiling Reviewed International coauthorship

    Lu Huo, Fange Liu, Hiroaki Iwaki, Tingfeng Li, Yoshie Hasegawa, Aimin Liu

    PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS   83 ( 1 )   178 - 187   2015.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/prot.24722

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  • Crystallographic and spectroscopic snapshots reveal a dehydrogenase in action Reviewed International coauthorship

    Lu Huo, Ian Davis, Fange Liu, Babak Andi, Shingo Esaki, Hiroaki Iwaki, Yoshie Hasegawa, Allen M. Orville, Aimin Liu

    NATURE COMMUNICATIONS   6   2015.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/ncomms6935

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  • Substrate profiling of cyclohexylamine oxidase and its mutants reveals new biocatalytic potential in deracemization of racemic amines Reviewed International coauthorship

    G. Li, J. Ren, H. Iwaki, D. Zhang, Y. Hasegawa, Q. Wu, J. Feng, P. C. K. Lau, D. Zhu

    Appl. Microbiol. Biotechnol.   98: 1681-1689   2014.4

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  • Isolation and Characterization of Marine Nonylphenol-Degrading Bacteria and Description of Pseudomaricurvus alkylphenolicus gen. nov., sp nov. Reviewed

    Hiroaki Iwaki, Makoto Fujioka, Yoshie Hasegawa

    CURRENT MICROBIOLOGY   68 ( 2 )   167 - 173   2014.2

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00284-013-0455-x

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  • Characterization of CpdC, a Large-Ring Lactone-Hydrolyzing Enzyme from Pseudomonas sp Strain HI-70, and Its Use as a Fusion Tag Facilitating Overproduction of Proteins in Escherichia coli Reviewed International coauthorship

    Yali Xu, Stephan Grosse, Hiroaki Iwaki, Yoshie Hasegawa, Peter C. K. Lau

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   79 ( 22 )   7091 - 7100   2013.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/AEM.02435-13

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  • Isolation and Characterization of a Marine Cyclohexylacetate-Degrading Bacterium Lutimaribacter litoralis sp nov., and Reclassification of Oceanicola pacificus as Lutimaribacter pacificus comb. nov. Reviewed

    Hiroaki Iwaki, Naoki Yasukawa, Makoto Fujioka, Kengo Takada, Yoshie Hasegawa

    CURRENT MICROBIOLOGY   66 ( 6 )   588 - 593   2013.6

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    DOI: 10.1007/s00284-013-0321-x

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  • Camphor Pathway Redux: Functional Recombinant Expression of 2,5-and 3,6-Diketocamphane Monooxygenases of Pseudomonas putida ATCC 17453 with Their Cognate Flavin Reductase Catalyzing Baeyer-Villiger Reactions Reviewed International coauthorship

    Hiroaki Iwaki, Stephan Grosse, Helene Bergeron, Hannes Leisch, Krista Morley, Yoshie Hasegawa, Peter C. K. Lau

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   79 ( 10 )   3282 - 3293   2013.5

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/AEM.03958-12

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  • Structural Analysis of a Novel Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A Reviewed International coauthorship

    I. Ahmad Mirza, David L. Burk, Bing Xiong, Hiroaki Iwaki, Yoshie Hasegawa, Stephan Grosse, Peter C. K. Lau, Albert M. Berghuis

    PLOS ONE   8 ( 3 )   2013.3

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    DOI: 10.1371/journal.pone.0060072

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  • Evidence for a Dual Role of an Active Site Histidine in alpha-Amino-beta-carboxymuconate-epsilon-semialdehyde Decarboxylase Reviewed International coauthorship

    Lu Huo, Andrew J. Fielding, Yan Chen, Tingfeng Li, Hiroaki Iwaki, Jonathan P. Hosler, Lirong Chen, Yoshie Hasegawa, Lawrence Que, Aimin Liu

    BIOCHEMISTRY   51 ( 29 )   5811 - 5821   2012.7

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    DOI: 10.1021/bi300635b

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  • Cloning, Baeyer-Villiger Biooxidations, and Structures of the Camphor Pathway 2-Oxo-Delta(3)-4,5,5-Trimethylcyclopentenylacetyl-Coenzyme A Monooxygenase of Pseudomonas putida ATCC 17453 Reviewed

    Hannes Leisch, Rong Shi, Stephan Grosse, Krista Morley, Helene Bergeron, Miroslaw Cygler, Hiroaki Iwaki, Yoshie Hasegawa, Peter C. K. Lau

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   78 ( 7 )   2200 - 2212   2012.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/AEM.07694-11

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  • Tropicibacter phthalicus sp nov., A Phthalate-Degrading Bacterium from Seawater Reviewed

    Hiroaki Iwaki, Ayaka Nishimura, Yoshie Hasegawa

    CURRENT MICROBIOLOGY   64 ( 4 )   392 - 396   2012.4

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00284-012-0085-8

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  • Isolation and characterization of marine bacteria capable of utilizing phthalate Reviewed

    Hiroaki Iwaki, Ayaka Nishimura, Yoshie Hasegawa

    WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY   28 ( 3 )   1321 - 1325   2012.3

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s11274-011-0925-x

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  • Maricurvus nonylphenolicus gen. nov., sp. nov., a nonylphenol-degrading bacterium isolated from seawater Reviewed

    Hiroaki Iwaki, Kengo Takada, Yoshie Hasegawa

    FEMS Microbiology Letters   327 ( 2 )   142 - 147   2012.2

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    Authorship:Lead author, Corresponding author   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    DOI: 10.1111/j.1574-6968.2011.02471.x

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  • Cyclohexylamine oxidase as a useful biocatalyst for the kinetic resolution and dereacemization of amines Reviewed International coauthorship

    Hannes Leisch, Stephan Grosse, Hiroaki Iwaki, Yoshie Hasegawa, Peter C. K. Lau

    CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE   90 ( 1 )   39 - 45   2012.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1139/V11-086

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  • Crystal Structures of Cyclohexanone Monooxygenase Reveal Complex Domain Movements and a Sliding Cofactor Reviewed International coauthorship

    I. Ahmad Mirza, Brahm J. Yachnin, Shaozhao Wang, Stephan Grosse, Helene Bergeron, Akihiro Imura, Hiroaki Iwaki, Yoshie Hasegawa, Peter C. K. Lau, Albert M. Berghuis

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   131 ( 25 )   8848 - 8854   2009.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ja9010578

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  • Isolation and characterization of new cyclohexylacetic acid-degrading bacteria Reviewed

    Hiroaki Iwaki, Emiko Nakai, Shota Nakamura, Yoshie Hasegawa

    CURRENT MICROBIOLOGY   57 ( 2 )   107 - 110   2008.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00284-008-9158-0

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  • Degradation of Monohydroxylated Benzoates by Strain KUFI-6N of the Yeast-like Fungus Exophiala jeanselmei Reviewed

    Hiroaki Iwaki, Tao Zhang, Yoshie Hasegawa

    World J Microbiol Biotechnol   24(2): 289-290   2008.1

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    Kansai University Research Grants

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  • Isolation and characterization of a new 2,4-dinitrophenol-degrading bacterium Burkholderia sp strain KU-46 and its degradation pathway Reviewed

    Hiroaki Iwaki, Kazuya Abe, Yoshie Hasegawa

    FEMS MICROBIOLOGY LETTERS   274 ( 1 )   112 - 117   2007.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1574-6968.2007.00816.x

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  • Characterization of a pseudomonad 2-nitrobenzoate nitroreductase and its catabolic pathway-associated 2-hydroxylaminobenzoate mutase and a chemoreceptor involved in 2-nitrobenzoate chemotaxis Reviewed International coauthorship

    Hiroaki Iwaki, Takamichi Muraki, Shun Ishihara, Yoshie Hasegawa, Kathryn N. Rankin, Traian Sulea, Jason Boyd, Peter C. K. Lau

    JOURNAL OF BACTERIOLOGY   189 ( 9 )   3502 - 3514   2007.5

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/JB.01098-06

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  • Degradation of 2-nitrobenzoate by Burkholderia terrae strain KU-15 Reviewed

    Hiroaki Iwaki, Yoshie Hasegawa

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   71 ( 1 )   145 - 151   2007.1

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1271/bbb.60419

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  • alpha-amino-beta-carboxymuconic-epsilon-semialdehyde decarboxylase (ACMSD) is a new member of the amidohydrolase superfamily Reviewed International coauthorship

    Tingfeng Li, Hiroaki Iwaki, Rong Fu, Yoshie Hasegawa, Hong Zhang, Aimin Liu

    BIOCHEMISTRY   45 ( 21 )   6628 - 6634   2006.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/bi060108c

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  • Pseudomonad cyclopentadecanone monooxygenase displaying an uncommon spectrum of Baeyer-Villiger oxidations of cyclic ketones Reviewed International coauthorship

    H Iwaki, SZ Wang, S Grosse, H Bergeron, A Nagahashi, J Lertvorachon, JZ Yang, Y Konishi, Y Hasegawa, PCK Lau

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   72 ( 4 )   2707 - 2720   2006.4

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/AEM.72.4.2707-2720.2006

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  • Kinetic and spectroscopic characterization of ACMSD from Pseudomonas fluorescens reveals a pentacoordinate mononuclear metallocofactor Reviewed International coauthorship

    TF Li, AL Walker, H Iwaki, Y Hasegawa, AM Liu

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   127 ( 35 )   12282 - 12290   2005.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ja0532234

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  • Cloning and Sequence Analysis of the 4-Hydroxybenzoate 3-Hydroxylase Gene from a Cyclohexanecarboxylate-degrading Gram-positive Bacterium, “Corynebacterium cyclohexanicum” Strain ATCC 51369 Reviewed

    Hiroaki Iwaki, Hiroshi Saji, Kazuya Abe, Yoshie Hasegawa

    Microbes and Environments   20 ( 3 )   144 - 150   2005

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1264/jsme2.20.144

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  • Degradation of Cyclopentanol by Trichosporon cutaneum Strain KUY-6A Reviewed

    Hiroaki Iwaki, Hiroshi Saji, Emiko Nakai, Yoshie Hasegawa

    Microbes and Environments   19 ( 3 )   241 - 243   2004

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1264/jsme2.19.241

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  • Monooxygenase-catalyzed Baeyer-Villiger oxidations: CHMO versus CPMO Reviewed International coauthorship

    SZ Wang, MM Kayser, H Iwaki, PCK Lau

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   22 ( 3-4 )   211 - 218   2003.6

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    DOI: 10.1016/S1381-1177(03)00036-5

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  • Prokaryotic homologs of the eukaryotic 3-hydroxyanthranilate 3,4-dioxygenase and 2-amino-3-carboxymuconate-6-semialdehyde decarboxylase in the 2-nitrobenzoate degradation pathway of Pseudomonas fluorescens strain KU-7 Reviewed International coauthorship

    T Muraki, M Taki, Y Hasegawa, H Iwaki, PCK Lau

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   69 ( 3 )   1564 - 1572   2003.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/AEM.69.3.1564-1572.2003

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  • Cyclohexanol biodegradation genes: A pathway of opportunities Reviewed

    H Iwaki, Y Hasegawa, M Teraoka, T Tokuyama, L Bernard, PCK Lau

    BIOCATALYSIS IN POLYMER SCIENCE   840   80 - 92   2003

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  • Cloning and characterization of a gene cluster involved in cyclopentanol metabolism in Comamonas sp strain NCIMB 9872 and biotransformations effected by Escherichia coli-expressed cyclopentanone 1,2-monooxygenase Reviewed

    H Iwaki, Y Hasegawa, SZ Wang, MM Kayser, PCK Lau

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   68 ( 11 )   5671 - 5684   2002.11

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/AEM.68.11.5671-5684.2002

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  • Baeyer-Villiger oxidations catalyzed by engineered microorganisms: Enantioselective synthesis of delta-valerolactones with functionalized chains Reviewed

    SZ Wang, G Chen, MM Kayser, H Iwaki, PCK Lau, Y Hasegawa

    CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE   80 ( 6 )   613 - 621   2002.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1139/V02-035

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  • Purification and characterization of cyclohexanone 1,2-monooxygenase from Exophiala jeanselmei strain KUFI-6N Reviewed

    Y Hasegawa, Y Nakai, T Tokuyama, H Iwaki

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   64 ( 12 )   2696 - 2698   2000.12

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  • A novel degradative pathway of 2-nitrobenzoate via 3-hydroxyanthranilate in Pseudomonas fluorescens strain KU-7 Reviewed International coauthorship

    Y Hasegawa, T Muraki, T Tokuyama, H Iwaki, M Tatsuno, PCK Lau

    FEMS MICROBIOLOGY LETTERS   190 ( 2 )   185 - 190   2000.9

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  • Cloning and expression of the N,N-dimethylformamidase gene from Alcaligenes sp strain KUFA-1 Reviewed

    Y Hasegawa, T Tokuyama, H Iwaki

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   63 ( 12 )   2091 - 2096   1999.12

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  • Identification of a transcriptional activetor (ChnR) and 6-oxohexanoate dehydrogenase (ChnE) in the cyclohexanol catabolic pathway in Acinetobacter sp. NCIMB 9871, and Localization of their encoding genes Reviewed International coauthorship

    H.Iwaki, Y.Hasegawa, M.Teraoka, T.Tokuyama, H.Bergeron, P.C.K.Lau

    Appl. Environ. Microbiol.   65巻11号 5158頁   1999.11

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  • Purification and characterization of a novel cyclohexylamine oxidase from the cyclohexylamine-degrading Brevibacterium oxydans IH-35A Reviewed

    H Iwaki, M Shimizu, T Tokuyama, Y Hasegawa

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   88 ( 3 )   264 - 268   1999.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

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  • Biodegradation of cyclohexylamine by Brevibacterium oxydans IH-35A Reviewed

    H Iwaki, M Shimizu, T Tokuyama, Y Hasegawa

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   65 ( 5 )   2232 - 2234   1999.5

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

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Books

MISC

  • キビタキの巣立ち雛

    岩木宏明

    Nature Study   71 ( 7 )   96   2025.7

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  • 吹田市と豊中市でヒメアカネを確認 Reviewed

    岩木宏明

    Nature Study   71 ( 4 )   50 - 51   2025.4

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  • 吹田市と豊中市でベニトンボを確認 Reviewed

    岩木宏明

    Nature Study   71 ( 2 )   18-19, 28   2025.2

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  • 吹田市と豊中市でクロヒバリモドキを確認

    岩木宏明

    Nature Study   71 ( 1 )   7   2025.1

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  • トラツグミ

    岩木宏明

    Nature Study   2025

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  • ツツドリ

    岩木宏明

    Nature Study   70 ( 10 )   125, 139   2024.10

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  • チョウトンボ

    岩木宏明

    Nature Study   69 ( 7 )   85, 96   2023.7

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  • ヒラアシキバチとオナガバチの1種

    岩木宏明

    Nature Study   69 ( 6 )   84   2023.6

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  • 雑種か新種か、巨大タンポポ

    岩木宏明

    Nature Study   68 ( 8 )   108   2022.8

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  • 蓮の花托にとまるタイワンウチワヤンマ

    岩木宏明

    Nature Study   68 ( 7 )   85, 90   2022.7

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  • 訪花するオスのタケクマバチ

    岩木宏明

    Nature Study   67 ( 11 )   152   2021.11

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  • 様々な花を訪花するタケクマバチ

    Nature Study   67 ( 11 )   168   2020.12

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  • アゲハチョウの交尾

    岩木宏明

    Nature Study   66 ( 10 )   140   2020.10

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  • 外来種 モンクチビルテントウ

    岩木宏明

    Nature Study   64 ( 11 )   168   2018.12

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  • 細菌によるニトロフェノールとニトロ安息香酸の分解

    岩木宏明, 長谷川喜衛

    技苑   No.116 15~22頁   15 - 22   2003.11

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    Language:Japanese   Publisher:関西大学  

    CiNii Books

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  • A gene encoding cyclododecanone monooxygenase

    H.Iwaki, Y.Hasegawa, PCK.Lau

    WO03025164, EP1430118, CA2460499   2003

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    The invention relates to a new strain of Pseudomonas putida (designated as HI-70) and to the isolation, cloning, and sequencing of a cyclododecanone monooxygenase-encoding gene (named cdnB) from said strain. The invention also relates to a new cyclododecanone monooxygenase and to a method of use of the cyclododecanone monooxygenase-encoding gene.

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  • Cloning, sequencing, and expression of a Comamonas cyclopentanone 1,2-monooxygenase encoding gene in Escherichia coli

    H.Iwaki, Y.Hasegawa, PCK.Lau

    WO0206452, CA2418155   2002

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    Cyclopentanone 1,2-monooxygenase (CPMO) from Comamonas (previously Pseudomonas) sp. strain NCIMB 9872 carries out the second step of a degradation pathway that allows the bacterium to use cyclopentanol as a sole carbon source for growth. In the present invention there is reported the localization of the CPMO-encoding gene (cpnB) on a 4.3-kb SphI fragment, the determination of its sequence. The 550-amino acid CPMO polypeptide (Mr, 62,111) encoded by the gene was found to have 36.5% identity with the sequence of cyclohexanone 1,2-monooxygenase (CHMO) of Acinetobacter sp. strain NCIMB 9871. The 62-kDa CPMO was expressed in E. coli as an IPTG-inducible protein.

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Presentations

  • 海洋細菌からの有用機能の探索

    岩木 宏明

    日本防菌防黴学会 第46回年次大会  2019.9 

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    Event date: 2019.9

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  • Pseudomonas sp. KU43P株のピペリジン分解系遺伝子の解析

    山本 泰誠, 岩木 宏明, 長谷川 喜衛

    日本農芸化学会 関西・中部支部 2019年度合同神戸大会  2019.9 

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  • 大腸菌を宿主としたBaeyer-Villigerモノオキシゲナーゼ異種発現系の構築

    山本 泰誠, 田端 美咲, 岩木 宏明, 長谷川 喜衛

    第23回関西大学先端科学技術シンポジウム  2019.1 

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    Event date: 2019.1

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  • Pseudomonas sp. KU-51 株のニトロフェノール分解系遺伝子の解析

    劉 雅軒, 岩木 宏明, 長谷川 喜衛

    第70回日本生物工学会大会  2018.9 

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    Event date: 2018.9

    Venue:吹田市(関西大学)  

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  • Paraburkholderia sp. KU-46株の2,4-DNP分解系遺伝子の解析

    山本 泰誠, 岩木 宏明, 長谷川 喜衛

    日本農芸化学会2018年度大会  2018.3 

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    Event date: 2018.3

    Venue:名古屋(名城大学)  

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  • 微生物機能を利用したバイオベースポリマー生産系の構築

    山本 泰誠, 岩木 宏明, 長谷川 喜衛

    第22回関西大学先端科学技術シンポジウム  2018.1 

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  • 海洋性細菌由来シクロアルカノンモノオキシゲナーゼ遺伝子の解析

    山本 泰誠, 岩木 宏明, ⻑谷川喜衛

    日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会(第49回講演会)  2017.9 

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    Event date: 2017.9

    Venue:大阪(大阪府立大学)  

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  • Exophiala jeanselmei KUFI-6N 株のシクロヘキサノンモノオキシゲナーゼ遺伝子の解析

    山本 泰誠, 岩木 宏明, 長谷川 喜衛

    第69回 (2017年度) 日本生物工学会大会  2017.9 

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    Event date: 2017.9

    Venue:東京(早稲田大学)  

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  • 海洋性テレフタル酸分解菌の分離と分解系遺伝子の解析

    澤岡 良太, 岩木 宏明, 長谷川 喜衛

    日本農芸化学会2017年度大会  2017.3 

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    Event date: 2017.3

    Venue:京都  

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  • Isolation and genetic characterization of cyclododecanone degrading bacterium

    2017.3 

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  • Burkholderia sp. KU-46 株による 4-ニトロ安息香酸お よび 4-アミノ安息香酸の分解

    田川 須美, 岩木 宏明, 長谷川 喜衛

    日本農芸化学会2017年度大会  2017.3 

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    Event date: 2017.3

    Venue:京都  

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  • Pseudomonas 属細菌のピペリジン分解系遺伝子の解析

    山本 泰誠, 岩木 宏明, 長谷川喜衛

    第21回 関西大学先端科学技術シンポジウム  2017.1 

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  • ピぺリジン分解菌の分離と分解系遺伝子の解析

    山本 泰誠, 岩木 宏明, 長谷川 喜衛

    2016年度(第68回)日本生物工学会大会  2016.9 

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    Event date: 2016.9

    Venue:富山  

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  • Burkholderia sp. KU-46 株の 2,4-ジニトロフェノール分解系遺伝子の解析

    小早 希, 岩木 宏明, 長谷川喜衛

    日本農芸化学会 2016年度(平成28年度)大会  2016.3 

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    Event date: 2016.3

    Venue:札幌  

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  • Corynebacterium cyclohexanicum MU株のシクロヘキサンカルボン酸分解系遺伝子の解析

    佐藤ゆう, 岩木 宏明, 長谷川喜衛

    日本農芸化学会 2016年度(平成28年度)大会  2016.3 

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    Event date: 2016.3

    Venue:札幌  

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  • 海洋性キシレン分解菌の分離と解析

    寺垣内将也, 岩木 宏明, 長谷川喜衛

    日本農芸化学会 2016年度(平成28年度)大会  2016.3 

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  • Burkholderia sp. KU-64株の2,6-ジニトロフェノール分解系の解析

    小早希, 岩木宏明, 長谷川喜衛

    日本防菌防黴学会 第42回年次大会  2015.9 

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    Event date: 2015.9

    Venue:千里ライフサイエンスセンター  

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  • Pseudomonas sp. HI-70株のシクロペンタデカノール分解系の解析

    佐藤ゆう, 岩木宏明, 長谷川喜衛

    日本防菌防黴学会 第42回年次大会  2015.9 

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    Event date: 2015.9

    Venue:千里ライフサイエンスセンター  

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  • 海洋性細菌 Marinobacter sp. KU31E株のトルエン分解系遺伝子の解析

    寺垣内将也, 長谷川喜衛, 岩木宏明

    第17回マリンバイオテクノロジー学会大会  2015.5 

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    Event date: 2015.5

    Venue:東京海洋大学品川キャンパス  

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  • Extending the Kynurenine Pathway to an Aldehyde Disarming Enzyme: Mechanistic Study of Bacterial AMSDH and Identification of the Correct Human Enzyme

    Ian Davis, Lu Huo, Fange Liu, Babak Andi, Shingo Esaki, Hiroaki Iwaki, Yoshie Hasegawa, Allen Orville, Aimin Liu

    FASEB JOURNAL  2015.4 

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    Event date: 2015.4

    Language:English  

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  • Extending the Kynurenine Pathway to an Aldehyde Disarming Enzyme: Mechanistic Study of Bacterial AMSDH and Identification of the Correct Human Enzyme

    H. Iwaki, L. Huo, F. Liu, B. Andi, S. Esaki, H. Iwaki, Y. Hasegawa, A.M. Orville, A. Liu

    American Society for Biochemistry and Molecularbiology 2015 Annual meeting  2015.3 

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    Event date: 2015.3

    Venue:Boston, MA  

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  • 2,6-ジニトロフェノール分解菌Burkholderia sp. KU-64株の分離と分解系遺伝子の解析

    小早希, 岩木宏明, 長谷川喜衛

    日本農芸化学会2015年度大会  2015.3 

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    Event date: 2015.3

    Venue:岡山  

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  • Pseudomonas sp. HI-70の大環状アルコール分解系の解析

    佐藤ゆう, 岩木宏明, 長谷川喜衛

    日本農芸化学会2015年度大会  2015.3 

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    Event date: 2015.3

    Venue:岡山  

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  • 海洋性フタル酸ジエチルヘキシル分解菌の分離と分解系遺伝子の解析

    小西健太, 岩木宏明, 長谷川喜衛

    日本農芸化学会2015年度大会  2015.3 

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    Venue:岡山  

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  • Pseudomonas fluorescens KU-7 株の2-ニトロ安息香酸走化性の代謝依存性

    藤岡諒, 岩木宏明, 長谷川喜衛

    2013年度 日本農芸化学会関西・中四国・西日本支部および日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会  2013.9 

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    Event date: 2013.9

    Venue:県立広島大学(広島)  

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  • 海洋性細菌のトルエン分解系遺伝子の解析

    國頭一平, 岩木宏明, 長谷川喜衛

    2013年度 日本農芸化学会関西・中四国・西日本支部および日本ビタミン学会近畿・中国四国・九州沖縄地区合同大会  2013.9 

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    Venue:県立広島大学(広島)  

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  • 環境アポトジェンを含む環境汚染物質の作用動態解析と化学生態学的防除法の開発プロジェクト

    土戸哲明, 池内俊彦, 上里新一, 下家浩二, 吉田宗弘, 福永健治, 安原裕紀, 長谷川喜衛, 老川典夫, 松村吉信, 岩木宏明

    技苑  2013.3 

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  • Pseudomonas fluorescens KU-7 株の2-ニトロ安息 香酸に対する走化性

    藤岡 諒, 岩木宏明, 長谷川喜衛

    日本農芸化学会2013年度大会  2013.3 

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    Event date: 2013.3

    Venue:仙台  

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  • 海洋性カンファー分解菌の分離とBaeyer-Villiger monooxygenase 遺伝子の取得

    安川直希, 岩木宏明, 長谷川喜衛

    日本農芸化学会2013年度大会  2013.3 

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    Venue:仙台  

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  • 海洋性細菌による芳香族化合物の分解

    高田健吾, 岩木宏明, 長谷川喜衛

    第17回関西大学先端科学技術シンポジウム  2013.1 

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    Event date: 2013.1

    Venue:関西大学, 吹田  

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  • 3-ヒドロキシルアントラニル酸の生産を目的とした 2-ニトロ安息香酸分解系の解析

    藤岡 諒, 岩木宏明, 長谷川喜衛

    第17回関西大学先端科学技術シンポジウム  2013.1 

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    Venue:関西大学, 吹田  

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  • Cupriavidus sp. KU-41株の2-ニトロ安息香酸分解系遺伝子の機能解析

    藤岡諒, 福島健斗, 岩木宏明, 長谷川喜衛

    第64回日本生物工学会大会  2012.10 

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    Event date: 2012.10

    Venue:神戸ポートピアホテル(兵庫県)  

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  • Pseudomonas fluorescens KU-7株の2-ニトロ安息香酸に対する走化性

    藤岡諒, 岩木宏明, 長谷川喜衛

    日本農芸化学会関西支部会2012年度大会  2012.9 

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    Event date: 2012.9

    Venue:京都学園大学  

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  • 環境アポトジェンを含む環境汚染物質の作用動態解析と化学生態学的防除法の開発プロジェクト

    土戸哲明, 池内俊彦, 上里新一, 下家浩二, 吉田宗弘, 福永健治, 安原裕紀, 長谷川喜衛, 老川典夫, 松村吉信, 岩木宏明

    技苑  2012.3 

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  • Burkholderia sp. KU-46株の2,4-ジニトロフェノール分解系遺伝子群の取得

    高田健吾 (D), 岩木宏明, 長谷川喜衛

    日本農芸化学会2012年度(平成24年度)大会  2012.3 

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    Venue:京都女子大学  

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  • 海洋性細菌におけるフタル酸分解系遺伝子の解析

    西村彩香 (D), 岩木宏明, 長谷川喜衛

    日本農芸化学会2012年度(平成24年度)大会  2012.3 

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    Venue:京都女子大学  

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  • 海洋性アルキルフェノール分解菌の分離と菌学的諸性質

    藤岡諒 (D), 高田健吾 (D), 安川直希 (B), 岩木宏明, 長谷川喜衛

    日本農芸化学会2012年度(平成24年度)大会  2012.3 

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    Venue:京都女子大学  

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  • 海洋性細菌Marinobacter sp. E-31株によるトルエンの分解

    城谷達也 (D), 岩木宏明, 長谷川喜衛

    日本農芸化学会2012年度(平成24年度)大会  2012.3 

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    Venue:京都女子大学  

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  • 海洋からの環境アポトジェン分解菌の単離と解析

    岩木宏明

    第16回 関西大学 先端科学技術シンポジウム  2012.1 

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    Event date: 2012.1

    Venue:関西大学, 吹田  

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  • 2Da03 Genetic analysis of 2-nitrobenzoate degradation pathway in Cupriavidus sp. strain KU-41

    Fujioka Makoto, Fukushima Kento, Iwaki Hiroaki, Hasegawa Yoshie

    2012 

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    Event date: 2012

    Language:Japanese  

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  • 海洋性フタル酸資化性菌の分離と解析

    西村彩香(D), 岩木宏明, 長谷川喜衛

    日本防菌防黴学会第 38 回年次大会  2011.8 

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    Event date: 2011.8

    Venue:千里ライフサイエンスセンター,豊中  

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  • 海洋性細菌によるトルエンの分解

    城谷達也(D), 岩木宏明, 長谷川喜衛

    日本防菌防黴学会第 38 回年次大会  2011.8 

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    Event date: 2011.8

    Venue:千里ライフサイエンスセンター,豊中  

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  • 海洋性細菌によるフェノールの分解

    高田健吾(D), 岩木宏明, 長谷川喜衛

    日本防菌防黴学会第 38 回年次大会  2011.8 

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    Event date: 2011.8

    Venue:千里ライフサイエンスセンター,豊中  

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  • 海洋性細菌による芳香族化合物の分解

    城谷達也(D), 岩木宏明, 長谷川喜衛

    日本農芸化学会2011年度大会  2011.3 

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    Event date: 2011.3

    Venue:京都女子大学  

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  • 海洋性シクロヘキシル酢酸分解菌の分離と特性

    斉藤潤(D), 岩木宏明, 長谷川喜衛

    日本農芸化学会2011年度大会  2011.3 

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    Event date: 2011.3

    Venue:京都女子大学  

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  • 海洋性細菌によるフタル酸の分解

    西村彩香(D), 岩木宏明, 長谷川喜衛

    日本農芸化学会2011年度大会  2011.3 

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    Event date: 2011.3

    Venue:京都女子大学  

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  • 海洋性フェノール分解菌の単離と解析

    高田健吾(D), 岩木宏明, 長谷川喜衛

    日本農芸化学会2011年度大会  2011.3 

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    Event date: 2011.3

    Venue:京都女子大学  

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  • 環境アポトジェンを含む環境汚染物質の作用動態解析と化学生態学的防除法の開発プロジェクト

    土戸哲明, 池内俊彦, 上里新一, 下家浩二, 吉田宗弘, 福永健治,, 安原裕紀, 長谷川喜衛, 老川典夫, 松村吉信, 岩木宏明

    技苑  2011.3 

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  • シクロヘキシル酢酸分解菌の単離と解析

    斉藤潤(D), 岩木宏明, 長谷川喜衛

    日本農芸化学会2010年度関西支部大会  2010.10 

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    Event date: 2010.10

    Venue:近畿大学農学部  

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  • The enzymatic oxidation of ketones and amines: a tale of two newly cloned classes of biocatalyst

    IWAKI,Hiroaki

    2010.9 

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    Event date: 2010.9

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  • 環境アポトジェンを含む環境汚染物質の作用動態解析と化学生態学的防除法の開発プロジェクト

    土戸哲明,, 池内俊彦, 上里新一, 下家浩二, 吉田宗弘, 福永健治, 安原裕紀, 長谷川喜衛, 老川典夫, 松村吉信, 岩木宏明

    技苑  2010.3 

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  • Sustained Development in Baeyer-Villiger Biooxidation Technology

    Peter C. K. Lau, Hannes Leisch, Brahm J. Yachnin, I. Ahmad Mirza, Albert M. Berghuis, Hiroaki Iwaki, Yoshie Hasegawa

    GREEN POLYMER CHEMISTRY: BIOCATALYSIS AND BIOMATERIALS  2010 

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    Event date: 2010

    Language:English  

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  • 新規2-ニトロ安息香酸分解菌の単離と分解系遺伝子の構造解析

    福島健斗, 岩木宏明, 長谷川喜衛

    日本微生物生態学会第25回大会  2009.11 

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    Event date: 2009.11

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  • 新規シクロヘキシル酢酸分解菌の単離と遺伝子解析

    宮下修治, 石破優, 福島健斗, 岩木宏明, 長谷川喜衛

    2009年度日本農芸化学会関西・中国・西日本支部、日本栄養・食糧学会九州・沖縄支部および日本食品化学工学会西日本支部合同沖縄大会  2009.10 

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    Event date: 2009.10

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  • Pseudomonas sp. HI-70 株のシクロドデカノール分解系遺伝子の取得と解析

    石破優, 福島健斗, 宮下修治, 岩木宏明, 長谷川喜衛

    2009年度日本農芸化学会関西・中国・西日本支部、日本栄養・食糧学会九州・沖縄支部および日本食品化学工学会西日本支部合同沖縄大会  2009.10 

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  • Cupriavidus sp. KU-41 株の2-ニトロ安息香酸分解系遺伝子の構造解析

    福島健斗, 石破優, 宮下修治, 岩木宏明, 長谷川喜衛

    2009年度日本農芸化学会関西・中国・西日本支部、日本栄養・食糧学会九州・沖縄支部および日本食品化学工学会西日本支部合同沖縄大会  2009.10 

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    Event date: 2009.10

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  • Burkholderia terrae KU-15 株の2- ニトロ安息香酸分解系遺伝子の解析

    岡村 憲治, 岩木宏明, 長谷川喜衛

    日本生物工学会学会第61回大会  2009.9 

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    Event date: 2009.9

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  • 3-ヒドロキシアントラニル酸の生産を目的とした2-ニトロ安息香酸分解系の解析

    福島健斗, 岡村憲治, 岩木宏明, 長谷川喜衛

    日本防菌防黴学会第36 回年次大会  2009.9 

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    Event date: 2009.9

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  • 環境アポトジェンを含む環境汚染物質の作用動態解析と化学生態学的防除法の開発プロジェクト

    土戸哲明, 池内俊彦, 下家浩二, 上里新一, 吉田宗弘, 福永健治, 安原裕紀, 長谷川喜衛, 岩木宏明, 老川典夫, 松村吉信

    技苑  2009.3 

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    Event date: 2009.3

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  • 環境汚染物質の微生物分解

    岩木宏明

    第13回関西大学先端科学技術シンポジウム  2009.1 

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    Event date: 2009.1

    Venue:関西大学  

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  • 2Ja11 Genetic analysis of 2-nitrobenzoate degradation pathway from Burkholderia terrae strain KU-15

    OKAMURA Kenji, IWAKI Hiroaki, HASEGAWA Yoshie

    2009 

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    Language:Japanese  

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  • P-154 新規2-ニトロ安息香酸分解菌の単離と分解系遺伝子の構造解析(ポスター発表)

    福島 健斗, 岩木 宏明, 長谷川 喜衛

    日本微生物生態学会講演要旨集  2009  日本微生物生態学会

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    Event date: 2009

    Language:Japanese  

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  • Burkholderia terrae KU-15株の2-ニトロ安息香酸分解系遺伝子の解析

    岡村憲治(D), 岩木宏明, 長谷川喜衛

    2008年度日本農芸化学会関西支部大会  2008.9 

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    Event date: 2008.9

    Venue:京都学園大学  

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  • Pseudomonas fluorescens KU-7株の2−ニトロ安息香酸分解系遺伝子の全容

    石原俊(D), 岩木宏明, 長谷川喜衛

    環境バイオテクノロジー学会 2007年度大会  2007.6 

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    Event date: 2007.6

    Venue:大阪大学  

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  • シクロヘキシル酢酸分解菌の分離

    中村将太(D), 岩木宏明, 長谷川喜衛

    環境バイオテクノロジー学会 2007年度大会  2007.6 

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    Event date: 2007.6

    Venue:大阪大学  

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  • Broad Substrate Spectrum and Crystal Structures of a New Baeyer-Villiger Cyclohexanone Monooxygenase of Rhodococcus Origin

    S. Wang, A. Mirza, B. Yachnin, A. Berghuis, H. Iwaki, Y. Hasegawa, A. Imura, S. Grosse, H. Bergeron, P.C.K. Lau

    90th Canadian Chemistry Conference and Exhibition in Winnipeg, Manitoba  2007.5 

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    Event date: 2007.5

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  • Exophiala jeanselmei KUFI-6N株による芳香族化合物の分解

    張涛(D), 長谷川喜衛, 岩木宏明

    平成18年度日本農芸化学会関西支部大会  2006.10 

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    Event date: 2006.10

    Venue:京都工芸繊維大学  

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  • Pseudomonas由来の2-アミノ-3-カルボキシムコン酸-6-セミアルデヒドデカルボキシラーゼの金属依存性

    石原俊(D), 長谷川喜衛, 岩木宏明

    平成18年度日本農芸化学会関西支部大会  2006.10 

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    Event date: 2006.10

    Venue:京都工芸繊維大学  

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  • Rhodococcus sp. KU-J株によるニトロシクロヘキサンの分解

    島田優輔(D), 長谷川喜衛, 岩木宏明

    平成18年度日本農芸化学会関西支部大会  2006.10 

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    Event date: 2006.10

    Venue:京都工芸繊維大学  

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  • Pseudomonas fluorescens KU-7株の2-ニトロ安息香酸に対する走化性

    石原俊(D), 岩木宏明, 長谷川喜衛

    平成18年度日本生物工学会大会  2006.9 

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    Event date: 2006.9

    Venue:大阪大学豊中キャンパス  

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  • Pseudomonas fluorescens KU-7株における2-ニトロ安息香酸分解系遺伝子の全体像

    長谷川喜衛, 村木孝充(D), 岩木宏明

    第10回関西大学先端科学技術シンポジウム  2006.1 

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    Event date: 2006.1

    Venue:関西大学  

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  • 1I16-5 Chemotaxis of Pseudomonas fluorescens strain KU-7 toward 2-nitrobenzoate

    ISHIHARA Shun, IWAKI Hiroaki, HASEGAWA Yoshie

    2006 

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    Event date: 2006

    Language:Japanese  

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  • Burkholderia sp. KU-15株による2-ニトロ安息香酸の分解

    世羅俊成(D), 岩木宏明, 長谷川喜衛

    平成17年度日本農芸化学会関西・中四国・西日本支部合同大会  2005.9 

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    Event date: 2005.9

    Venue:大阪大学  

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  • Burkholderia sp. KU-46株による2,4-ジニトロフェノールの分解

    阿部和也(D), 岩木宏明, 長谷川喜衛

    平成17年度日本農芸化学会関西・中四国・西日本支部合同大会  2005.9 

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    Event date: 2005.9

    Venue:大阪大学  

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  • Ralstonia sp. KUA-1 株によるシクロヘキシル酢酸の分解

    中井恵美子(B), 佐治洋(D), 岩木宏明, 長谷川喜衛

    平成16年度日本生物工学会大会  2004.9 

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    Event date: 2004.9

    Venue:名城大学  

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  • Corynebacterium cyclohexanicum MU 株によるCyclohexanecarboxylic acid の分解

    佐治洋(D), 永橋文子(D), 岩木宏明, 長谷川善衛

    平成16年度日本農芸化学会大会  2004.3 

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    Event date: 2004.3

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  • Pseudomonas fluorescens KU-7株の2-ニトロ安息香酸分解系遺伝子のクローニングと解析

    村木孝充, 岩木宏明, 長谷川喜衛, PCK.Lau

    平成16年度日本農芸化学会大会  2004.3 

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    Event date: 2004.3

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  • Biocatalysts and microorganisms as environmental and sustainability tools

    P.C.K.Lau, H.Bergeron, J.Boyd, S.Grosse, S.Z.Wang, J.Z.Yang, K.Rankin, T.Sulea, A.Imura, A.Miyadera, H.Iwaki, T.Muraki, Y.Hasegawa

    2004.1 

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    Event date: 2004.1

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  • 2E14-2 Degradation of cyclohexylacetic acid by Ralstonia sp. strain KUA-1

    NAKAI Emiko, SAJI Hiroshi, IWAKI Hiroaki, HASEGAWA Yoshie

    2004 

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    Event date: 2004

    Language:Japanese  

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  • A REACT-IRTM ANALYSIS OF LAURYL LACTONE PRODUCTION BY CYCLODODECANONE MONOOXYGENASE DERIVED FROM Pseudomonas putida STRAIN HI-70

    Ayako Nagahashi, Hiroaki Iwaki, Yoshie Hasegawa, Jianzhong Yang, Stephan Grosse, Helene Bergeron, Jason Boyd, Peter C. K. Lau

    Pseudomonas 2003  2003.9 

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    Event date: 2003.9

    ReactIRTM 4000 is a state-of-the-art tool that allows realtime, in-situ analysis of a biotransformation and it can provide a characteristic fingerprint of specific molecularinformation that reflects changes in a reaction as a function of time. We have applied this technique to monitor the progress of a Baeyer-Villiger monooxygenase (BVMO) reaction. BVMOs areflavoproteins and they are nature's alternative to the abiotic Baeyer-Villiger organic reaction transformations of (cyclic) ketones into esters or lactones catalyzed by peracids. The latter chemicals are strong oxidants and can produce undesirable side reactions. BVMO applicationsinclude the synthesis of natural products andproduction of chiral building blocks for possible drug synthesis and value-added products. As part of a biocatalyst ”toolkit” development program, we have cloned a cyclododecanone monooxygenase (CDMO)-encoding gene (cdnB) from a soil microorganism, Pseudomonas putida strain HI-70. In a phylogenetic analysis, the predicted amino acid sequence of CDMO is found in a different cluster than that of the majority of the cloned BVMOs (e.g. cyclohexanone monooxygenase [CHMO] of Acinetobacter spp; cyclopentanone monooxygenase [CPMO] of Comamonas sp. NCIMB 9872). CDMO is active towards cyclic ketones, ranging from C10 to C16. This is different from the specificity shown by CHMO or CPMO that favours short-chain cyclic compounds. Using an overexpression clone of cdnB gene (pCD100B) in Escherichia coli strain BL21, the production of lauryl lactone from cyclododecanone was evaluated. ReactIR was found to be an useful monitoring tool in the development of this biocatalytic process. This study includes also a preliminary development of a two-phase bioreactor system that addresses low reactant solubility and product recovery.

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  • CLONING AND CHARACTERIZATION OF THE INITIAL 2-NITROBENZOATE NITROREDUCTASE AND 2-HYDROXYLAMINOBENZOATE MUTASE ENCODING GENES FOR 2-NITROBENZOATE CATABOLISM IN PSEUDOMONAS FLUORESCENS STRAIN KU-7

    Takamichi Muraki, Ayako Nagahashi, Masami Taki, Hiroaki Iwaki, Yoshie Hasegawa, Peter C.K. Lau

    Pseudomonas 2003  2003.9 

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    Event date: 2003.9

    2-nitrobenzoic acid (2-NBA) via the novel formation of 3-hydroxyanthranilic acid (3-HAA) instead of anthranilate or 2-aminobenzoate as anintermediate. We previously cloned and analyzed the 3-HAA catabolic gene cluster (nbaEXHJIGFCD), responsible for the conversion of 3-HAA into Krebs cycle intermediate (Muraki et al., Appl. Environ. Microbiol., 69:1564-1572, 2003). In order to identify the initial genes of the nbapathway, we further screened 24,000 transconjugants and isolated 37 mutants strains that were unable to grow on solid medium containing 2-NBA as a sole source of carbon and energy. DNA regions flanking the transposon in these mutant strains were isolated by Inverse PCR and individual DNA sequences were determined. As a result, we identified two mutant strains, named KUM-19 and KUM-33, in which the potential nbaA and nbaB genes were mutagenized respectively. The predicted primary structure of the nbaAencoded protein was found to have 31% sequence identity to a FMN-binding protein (MTH152, PDB ID: 1EJE) of Methanobacterium thermoautrophicum dH. The predicted amino acid sequence of the nbaB-encoded protein was found to have 34% sequence identity to the 4-hydroxylaminobenzoate lyase (PnbB) of 4-nitrobenzoate assimilating Pseudomonas putida TW3. Pairwise alignment of NbaA and MTH152 sequences indicated the presence of 7 conserved amino acid residues that may be involved in binding of FMN molecule. The nbaA and nbaB ORFs were separately cloned into the IPTG-inducible expression vector pSD80, and crude extracts of transformed E. coli cells werefound to show expression of the expected molecular size as well as the expected activity of 2-nitrobenzoate nitroreductase (NbaA) and 2-hydroxylaminobenzoate mutase (NbaB). In summary, at least ten catabolic genes and one regulatory gene are necessary for the metabolism of 2-NBA in strain KU-7. Within the 23-kb sequenced locus there are at least 13 other ORFs, many of which remain to be characterized.

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  • A ReactIR study of cyclododecanone monooxygenase-catalyzed reaction for lauryl lactone production

    J.Yang, H.Iwaki, H.Bergeron, PCK.Lau

    Annual general meeting, Canadian Society of Microbiologists  2003.5 

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  • 3-hydroxyanthranilate 3,4-dioxygenase, a novel eukaryotic homolog in the degradation pathway of 2-nitrobenzoate by Pseudomonas fluorescens strain KU-7

    T.Muraki, M.Taki, Y.Hasegawa, H.Iwaki, P.C.K.Lau

    American Society for Microbiology  2001.10 

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    Event date: 2001.10

    3-hydroxyanthranilate 3,4-dioxygenase, a novel eukaryotic homolog in the degradation pathway of 2-nitrobenzoate by Pseudomonas fluorescens strain KU-7

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  • Cyclopentanone 1,2-monooxygenase:Old wine, new bottle

    H. Iwaki, Y. Hasegawa, S.Wang, M.Kayser, P.C. Lau

    ASM conference on Biodegradation, Biotransformation, and Biocatalysis  2001.10 

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    Event date: 2001.10

    Cyclopentanone 1,2-monooxygenase:Old wine, new bottle

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  • Biodegradation of cycloaliphatic compounds: New genes and opportunities

    H.Iwaki, Y.Hasegawa, PCK. Lau

    Pseudomonas 2001  2001.9 

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    Event date: 2001.9

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  • A fistful of BVMOs (Baeyer-Villiger monooxygenase)

    H.Iwaki, Y.Hasegawa, T.Tokuyama, A.Imura, H.Bergeron, PCK.Lau

    IBC's 5th Annual World Congress on Enzyme Technologies 2000  2000.2 

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    Event date: 2000.2

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  • Cloning and analysis of cyclohexylamine-degrading genes from Brevibacterium oxydans IH-35A.

    Iwaki Hiroaki, Ohashi Yumiko, Tokuyama Tai, Hasegawa Yoshie

    2000 

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    Event date: 2000

    Language:Japanese  

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  • Gene cloning and enzyme characterization of cyclohexanone monooxygenase from Rhodbcoccus maris Hl-31.

    Iwaki Hiroaki, Hasegawa Yoshie, Tokuyama Tai

    1999 

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    Event date: 1999

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  • Cloning and analysis of cyclohexanol-degrading genes from Rhodococcusmaris HI-31.

    Nishida Chisato, Iwaki Hiroaki, Hasegawa Yoshie, Tokuyama Tai

    1999 

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    Event date: 1999

    Language:Japanese  

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  • Purification and Characterization of Cyclohexylamine Oxidase from Brevibacterium oxydans IH-35A

    Shimizu Masatake, Iwaki Hiroaki, Hasegawa Yoshie, Tokuyama Tai

    1999 

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    Event date: 1999

    Language:Japanese  

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  • Cloning of the N,N-Dimethylformamidase (DMFase) gene from Alcaligenes sp. KUFA-1.

    Iwaki Hiroaki, Matuo Masaki, Hasegawa Yoshie, Tokuyama Tai, Lau Peter C. K.

    1998 

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    Event date: 1998

    Language:Japanese  

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  • Purification and characterization of cyclohexanone monooxygenase(CHMO) from Rhodococcus sp. Hl-31.

    Matuo Masaki, Iwaki Hiroaki, Hasegawa Yoshie, Tokuyama Tai

    1998 

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    Event date: 1998

    Language:Japanese  

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  • Analysis of cyclohexanol degradation genes of Acinetobacter sp. NCIMB9871. : Analysis of expressionsytemin chnA and chnB

    Teraoka Masahiro, Iwaki Hiroaki, Hasegawa Yoshie, Tokuyama Tai, Lau Peter C. K.

    1998 

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    Event date: 1998

    Language:Japanese  

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  • Cloning of p-hydroxybenzoate hydroxylase gene from Trichosporon cutaneum KUY-6A

    Teraoka Masahiro, Iwaki Hiroaki, Uotome Atsushi, Hasegawa Yoshie, Tokuyama Tai

    1997 

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    Event date: 1997

    Language:Japanese  

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  • Analysis of cyclohexanol degradation genes of Acinetobacter sp. NCIB 9871. : Cloning of the genes coding for the conversion of ε-caprolactone to adipate.

    Ishikawa Ayane, Iwaki Hiroaki, Teraoka Masahiro, Hasegawa Yoshie, Tokuyama Tai, Lau Peter C. K.

    1997 

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    Event date: 1997

    Language:Japanese  

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  • Cloning of upstream and downstream of cyclohexanone monooxygenase gene from Acinetobacter calcoaceticus NCIB 9871

    Iwaki Hiroaki, Hasegawa Yoshie, Hashimoto Yasuyuki, Tokuyama Tai

    1996 

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    Event date: 1996

    Language:Japanese  

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  • アンチセンスペプチド核酸を用いた減算的な菌叢改変手法の開発

    岡野 憲司, 日詰 達哉, 佐藤 悠, 岩木 宏明, 本田 孝祐

    2022.10 

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    Venue:Web開催  

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  • 軟質塩ビシートの硬化劣化への土壌細菌の関与

    平田 悠大, 別宮 浩之, 天牛 英清, 八木 敬祐, 岡野 憲司, 岩木 宏明

    2022.10 

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    Venue:Web開催  

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  • ナノ構造が発現する殺菌メカニズムの解明~オートリシスの影響について~

    伊藤 健, 三村 爽馬, 清水 智弘, 新宮原 正三, 岩木 宏明

    2022.3 

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  • Exophiala jeanselmei KUFI-6N 株由来シクロアルカノン モノオキシゲナーゼの解析

    小林 健人, 山本 泰誠, 長谷川 喜衛, 岩木 宏明

    2021.10 

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Works

  • 第115回醗酵学懇話会 ~培養技術の温故知新(基礎から応用まで)~

    岩木 宏明

    2019.8

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  • 第112回醗酵学懇話会

    岩木 宏明

    2018.1

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  • 第111回醗酵学懇話会~クラフトビールを知る・楽しむ~

    岩木 宏明

    2017.8

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  • 第68回日本生物工学会大会シンポジウム:学会活動が先導する実用化研究・技術~学会は産官学の出会いの場となれるか~

    中澤 昌美, 岩木 宏明, 大橋 正孝, 小高 敦史

    2016.9

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Research Projects

  • プロドラッグ合成を指向した微生物によるアミン化合物のγ-グルタミル化の解析

    Grant number:22K05418  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    岩木 宏明

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    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

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  • Analysis of interaction between single cell and nanostructured surface to clarify the sterilization mechanism due to nanostructure

    Grant number:21H01773  2021.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Grant amount:\17290000 ( Direct Cost: \13300000 、 Indirect Cost:\3990000 )

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  • Development of synthesis of waste edible oil-based polymers with microorganisms

    Grant number:17K07735  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Iwaki Hiroaki

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    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    The aim of this study was to screen genes that can be utilized to construct a polymer production system with waste edible oil as raw material. One major component of edible oil is oleic acid. We investigated genes encoding cyclooctanone monooxygenase, an enzyme that can synthesize poly(8-octanolide) from oleic acid via cyclooctanone and 8-octanolide. Additionally, we screened for cyclohexanecarboxylic acid-degrading genes from the aromatization pathway. The encoded enzymes can synthesize 4-hydroxycyclohexanecarboxylic acid, 4-hydroxybenzoic acid, protocatechuic acid, and 2-pyrone-4,6-dicarboxylic acid from oleic acid via cyclohexanecarboxylic acid.

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  • Genetic analysis of chemotactic receptors in bacteria

    Grant number:22780077  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    IWAKI Hiroaki

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    Grant amount:\2990000 ( Direct Cost: \2300000 、 Indirect Cost:\690000 )

    We analyzed the mechanism of chemotaxis of Pseudomonas fluorescens strain KU-7 toward 2-nitrobenzoate. We observed that the chemotaxis of strain KU-7 to 2-nitrobenzoate is metabolism-dependent and that the chemoreceptor gene nbaY is induced by growth on 2-nitrobenzoate. Furthermore, the genes responsible for 2-nitrobenzoate metabolism, including nbaY, were found to be induced by the metabolic intermediate 2-amino-3- carboxymuconate-6-semialdehyde rather than 2-nitrobenzoate.

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  • Development of asymmetric syntheses and valuable bioactive compounds based on alkenylphosphonates

    Grant number:15590031  2003 - 2005

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    IWAKI Hiroaki

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    Grant amount:\2700000 ( Direct Cost: \2700000 )

    Phosphates are vital components in our body as an intermediate of the energy storage and the intracellular signaling. Phosphonate derivatives are analogues of phosphates and can be act as agonists or antagonists of the biochemical reactions mediated by phosphates. Some of them have already been used as real drugs. On the other hand, from the synthetic organic chemistry point of view, phosphonates are versatile as substrates for an olefin forming reaction, Horner-Wadsworth-Emmons reaction, and also as synthetic intermediates of chiral phosphine ligands for the transition metal catalysis. Thus, in the study based on phosphonates, we can expect the development of the research in which both synthetic organic chemistry and biological chemistry are combined together. The purpose of this study is the creation of useful physiologically active compounds based on phosphonates using synthetic technology we have developed.
    In the present study, I focused on the development of immunosuppressive agent. An immunosuppressant FTY-720 is known to be activated by phosphorylation in one of its hydroxyl groups. The activated FTY-720 binds to sphingosine-1-phosphate receptor and cause the homing of the lymphocytes to lymph node. Thereby, I expected the increase of the activity with the application of phosphorilated FTY-720. The phosphonates, however, likely to be hydrolysed in the body. It is, therefore, we synthesized cyclic phosphates as a stable phosphorilated derivatives of FTY-720 in this study. Cyclic phosphates are not only stable but also non-asymmetric compounds which is advantageous for the further development for drugs. We also synthesized phosphonate derivatives of FTY-720. The immunosuppressive activity of the derivatives is being evaluated.

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Teaching materials

  •  特になし

Teaching method presentations

  • 第38回生涯学習吹田市民大学関西大学講座総合テーマ「くらしと微生物」テーマ「微生物と遺伝子」

Special notes on other educational activities

  • 関西大学サイエンスセミナー2006年~2010年