Updated on 2025/09/30

写真a

 
SASAKI,Miho
 
Organization
Faculty of Chemistry, Materials and Bioengineering Associate Professor
Title
Associate Professor
Homepage
http://kaken.nii.ac.jp/d/r/70581031.ja.html
External link

Degree

  • Engineering ( Kansai University )

Research Interests

  • Environmental risk

  • 環境浄化

  • Bioremediation

  • 環境

  • Biomass

  • 抗菌剤

  • antibiotics

  • Microbial control

  • Microorganism

  • 有用物質生産

  • Biorefinery

  • Antimicrobial resistance

  • Biofilm

  • sterilization

Research Areas

  • Life Science / Applied microbiology

  • Life Science / Ecology and environment

  • Life Science / Hygiene and public health (laboratory)

  • Environmental Science/Agriculture Science / Chemical substance influence on environment

  • Environmental Science/Agriculture Science / Environmental impact assessment

Professional Memberships

  • THE SOCIETY FOR BIOTECHNOLOGY, JAPAN

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  • JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

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  • THE SOCIETY FOR ANTIBACTERIAL AND ANTIFUNGAL AGENTS, JAPAN

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Committee Memberships

  • 日本防菌防黴学会   和文誌編集委員  

    2023.4 - Present   

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    Committee type：Academic society

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  • 日本防菌防黴学会   評議員  

    2017.4 - Present   

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    Committee type：Academic society

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  • 日本防菌防黴学会   Biocontrol Science編集委員  

    2015.4 - 2023.3   

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    Committee type：Academic society

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Papers

  • Effect of effective microorganisms on water purification and microbial community in a pond

    Miho Mori, Shouhei Miki, Masaki Inayoshi, Masaki Yamamoto, Ryota Ozaki, Yoshikazu Sakagami, Toru Jojima

    CLEAN – Soil, Air, Water   52 ( 7 )   2024.6

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    Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    Abstract

    There are very few reports about water purification and its influence on indigenous microorganisms by effective microorganisms (EM) in the fields. This study investigated the effect of EM on water purification and microbial community via in vitro and field experiments. In in vitro water purification experiments, we examined values for chemical oxygen demand (COD), total phosphorus, and total nitrogen (TN). In systems in which the active EM solutions were at a concentration of less than 1:2000 of the total water sample, the values after 28 days were equal to or lower than those of the control. In systems using 1:200 and 1:100 active EM solutions, COD and TN values were significantly lower in artificial wastewater compared to control. Conversely, they were significantly higher than the control in the pond water. When the active EM solutions were added to the pond water, total microbial plate counts were higher than the control after 28 days for all concentrations of the EM active solutions. Excessive use of EM caused deterioration of water quality and increase of microbial counts. The results of field experiments for 4 years suggested that EM treatment did not purify the pond water, establish introduced EM, or affect indigenous microorganisms.

    DOI： 10.1002/clen.202300353

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  • Collibacillus ludicampi gen. nov., sp. nov., a new soil bacterium of the family Alicyclobacillaceae. Reviewed International journal

    Toru Jojima, Yuki Ioku, Yasuhisa Fukuta, Norifumi Shirasaka, Yoshinobu Matsumura, Miho Mori

    International journal of systematic and evolutionary microbiology   73 ( 5 )   2023.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1099/ijsem.0.005827

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  • A Silent Operon of Photorhabdus luminescens Encodes a Prodrug Mimic of GTP Reviewed

    Negar Shahsavari, Boyuan Wang, Yu Imai, Miho Mori, Sangkeun Son, Libang Liang, Nils Böhringer, Sylvie Manuse, Michael F. Gates, Madeleine Morrissette, Rachel Corsetti, Josh L. Espinoza, Chris L. Dupont, Michael T. Laub, Kim Lewis

    mBio   13 ( 3 )   2022.6

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    Publishing type：Research paper (scientific journal)   Publisher：American Society for Microbiology  

    Drug-resistant Gram-negative bacteria have become the major problem driving the antimicrobial resistance crisis. Searching outside the overmined actinomycetes, we focused on Photorhabdus , gut symbionts of enthomopathogenic nematodes that carry up to 40 biosynthetic gene clusters coding for secondary metabolites.

    DOI： 10.1128/mbio.00700-22

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  • Analysis of the Relationship of Microbial Contamination with Temperature and Cleaning Frequency and Method of Domestic Refrigerators in Japan. Reviewed International journal

    Miho Mori, Yoshikazu Sakagami, Megumi Tanaka, Ryo Inoue, Toru Jojima

    Journal of food protection   83 ( 7 )   1234 - 1240   2020.7

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    Language：English   Publishing type：Research paper (scientific journal)  

    ABSTRACT: Hygiene management of domestic refrigerators is an important aspect of food poisoning prevention. The aim of the present study was to confirm the relationship between microbial contamination and hygiene management by measuring microbial levels and investigating temperature and cleaning frequency and method of domestic refrigerators in Japan. We analyzed three internal sections (the egg compartment, bottom shelf, and vegetable drawer) of 100 domestic refrigerators in Japan. Salmonella, Listeria monocytogenes, and Yersinia enterocolitica were not found in any of the refrigerators, but coliforms and Escherichia coli were detected in more than one household, and Staphylococcus aureus was the most frequently isolated pathogen. The prevalences of these microorganisms had similar tendencies in all three sections sampled and were highest in the vegetable drawer. The temperature distribution in the refrigerators was also investigated, and a temperature >6.1°C (improper temperature) was found in 46.2% of the areas surveyed. Only 17% of the respondents cleaned their refrigerators monthly or more often, and this frequency was lower than that reported in other countries. Fifty percent of the respondents used only water to clean the refrigerator, 10% used only an alcohol or disinfecting wipe, and 8% used only a dry cloth. Although no significant correlations were found between microbial contamination and temperatures in refrigerators, correlations were found between microbial contamination and refrigerator cleaning frequency and/or method. To our knowledge, this is the first detailed survey concerning relationships between microbial contamination and hygiene management in domestic refrigerators in Japan. The data obtained can be used to promote food poisoning management in Japanese households.

    DOI： 10.4315/0362-028X.JFP-19-322

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  • A new antibiotic selectively kills Gram-negative pathogens. Reviewed

    Imai Y, Meyer KJ, Iinishi A, Favre-Godal Q, Green R, Manuse S, Caboni M, Mori M, Niles S, Ghiglieri M, Honrao C, Ma X, Guo J, Makriyannis A, Linares-Otoya L, Böhringer N, Wuisan ZG, Kaur H, Wu R, Mateus A, Typas A, Savitski MM, Espinoza JL, O'Rourke A, Nelson KE, Hiller S, Noinaj N, Schäberle TF, D'Onofrio A, Lewis K

    Nature   576 ( 7787 )   459 - 464   2019.11

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1038/s41586-019-1791-1

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    Other Link： http://www.nature.com/articles/s41586-019-1791-1

  • Evaluation of the influence of sprinkling powdered slaked lime on microorganisms for the prevention of domestic animal infectious diseases. Reviewed

    Mori M, Sakagami Y, Hamazaki Y, Jojima T

    Environmental technology   40 ( 23 )   3094 - 3104   2019.9

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Taylor and Francis Ltd.  

    DOI： 10.1080/09593330.2018.1465128

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  • Comparative ecotoxicity of imidacloprid and dinotefuran to aquatic insects in rice mesocosms Reviewed

    Koji Kobashi, Takaaki Harada, Yoshihiro Adachi, Miho Mori, Makoto Ihara, Daisuke Hayasaka

    ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY   138   122 - 129   2017.4

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    DOI： 10.1016/j.ecoenv.2016.12.025

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  • Bacterial and Fungal Counts of Dried and Semi-Dried Foods Collected from Dhaka, Bangladesh, and Their Reduction Methods Reviewed

    Farahnaaz Feroz, Hiromi Shimizu, Terumi Nishioka, Miho Mori, Yoshikazu Sakagami

    BIOCONTROL SCIENCE   21 ( 4 )   243 - 251   2016.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.4265/bio.21.243

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  • Bacterial and Fungal Loads in Raw Sea Foods, Fruits and Vegetables collected from Dhaka, Bangladesh and the Effect of Heat on its Growth. Reviewed

    Farahnaaz Feroz, Hiromi Shimizu, Miho Mori, Yoshikazu Sakagami

    Bangladesh Journal of Microbiology   33 ( 1-2 )   23 - 28   2016.12

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  • Draft genome sequence of the basidiomycetous fungus Flammulina velutipes TR19 Reviewed

    Atsushi Kurata, Yasuhisa Fukuta, Miho Mori, Noriaki Kishimoto, Norifumi Shirasaka

    Genome Announcements   4 ( 3 )   2016.6

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Society for Microbiology  

    DOI： 10.1128/genomeA.00505-16

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  • A new carotenoid, 9Z,9 ' Z-tetrahydroastaxanthin, from the sea cucumber Plesiocolochirus minutus Reviewed

    Takashi Maoka, Syu Nakachi, Ryouhei Kobayashi, Miho Mori, Yoshikazu Sakagami

    TETRAHEDRON LETTERS   56 ( 43 )   5954 - 5955   2015.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.tetlet.2015.09.060

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  • Bioremediation of Bisphenol-A Polluted Soil by Sphingomonas bisphenolicum AO1 and the Microbial Community Existing in the Soil Reviewed

    Yoshinobu Matsumura, Ayako Akahira-Moriya, Miho Sasaki-Mori

    BIOCONTROL SCIENCE   20 ( 1 )   35 - 42   2015.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.4265/bio.20.35

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  • Metabolic engineering for improved production of ethanol by Corynebacterium glutamicum Reviewed

    Toru Jojima, Ryoji Noburyu, Miho Sasaki, Takahisa Tajima, Masako Suda, Hideaki Yukawa, Masayuki Inui

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   99 ( 3 )   1165 - 1172   2015.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-014-6223-4

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  • Characterization of Microorganisms Isolated from the Black Dirt of Toilet Bowls and Componential Analysis of the Black Dirt Reviewed

    Miho Mori, Yusuke Nagata, Kazuma Niizeki, Mitsuhiro Gomi, Yoshikazu Sakagami

    BIOCONTROL SCIENCE   19 ( 4 )   173 - 179   2014.12

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    DOI： 10.4265/bio.19.173

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  • Biofilm-Forming Activity of Bacteria Isolated from Toilet Bowl Biofilms and the Bactericidal Activity of Disinfectants against The Isolates Reviewed

    Miho Mori, Mitsuhiro Gomi, Norihiko Matsumune, Kazuma Niizeki, Yoshikazu Sakagami

    BIOCONTROL SCIENCE   18 ( 3 )   129 - 135   2013.9

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    DOI： 10.4265/bio.18.129

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  • Synergistic Bactericidal Effects of a Sublethal Concentration of Didecyldimethylammonium chloride (DDAC) and Low Concentrations of Nonionic Surfactants against Staphylococcus aureus Reviewed

    Mitsuhiro Gomi, Yukihiko Osaki, Miho Mori, Yoshikazu Sakagami

    BIOCONTROL SCIENCE   17 ( 4 )   175 - 181   2012.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.4265/bio.17.175

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  • Identification of Carotenoids in the Freshwater Shellfish Unio douglasiae nipponensis, Anodonta lauta, Cipangopaludina chinensis laeta, and Semisulcospira libertina Reviewed

    Takashi Maoka, Junko Ochi, Miho Mori, Yoshikazu Sakagami

    JOURNAL OF OLEO SCIENCE   61 ( 2 )   69 - 74   2012.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.5650/jos.61.69

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  • Carotenoids in Marine Invertebrates Living along the Kuroshio Current Coast Reviewed

    Takashi Maoka, Naoshige Akimoto, Miyuki Tsushima, Sadao Komemushi, Takuma Mezaki, Fumihito Iwase, Yoshimitsu Takahashi, Naomi Sameshima, Miho Mori, Yoshikazu Sakagami

    MARINE DRUGS   9 ( 8 )   1419 - 1427   2011.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3390/md9081419

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  • Identification of mannose uptake and catabolism genes in Corynebacterium glutamicum and genetic engineering for simultaneous utilization of mannose and glucose Reviewed

    Miho Sasaki, Haruhiko Teramoto, Masayuki Inui, Hideaki Yukawa

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   89 ( 6 )   1905 - 1916   2011.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-010-3002-8

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  • Research on the Antibacterial Activity of Silver Compounds against Some Bacteria Living in theToilet Environment Reviewed

    坂上吉一, 袋瀬直晃, 森美穂, 五味満裕, 大崎幸彦, 懸橋里枝, 山村伸吾

    防菌防黴学会誌   39 ( 1 )   3 - 11   2011.1

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

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  • Antibacterial Efficacy and Mechanisms of the Bactericidal Activity of HM-242, a New Antiseptic against Staphylococcus aureus Reviewed

    奥西淳二, 西村公志, 高田輝, 山口羽衣子, 森美穂, 前田四郎, 前田拓也, 西原力, 米虫節夫, 坂上吉一

    防菌防黴学会誌   38 ( 8 )   501 - 508   2010.8

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  • Xylitol production by recombinant Corynebacterium glutamicum under oxygen deprivation Reviewed

    Miho Sasaki, Toru Jojima, Masayuki Inui, Hideaki Yukawa

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   86 ( 4 )   1057 - 1066   2010.4

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    DOI： 10.1007/s00253-009-2372-2

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  • Isolation and Characterization of Novel Bisphenol - A-Degrading Bacteria from Soils Reviewed

    Yoshinobu Matsumura, Chiemi Hosokawa, Miho Sasaki-Mori, Ayako Akahira, Kenji Fukunaga, Toshihiko Ikeuchi, Ko-Ichi Oshiman, Tetsuaki Tsuchido

    BIOCONTROL SCIENCE   14 ( 4 )   161 - 169   2009.12

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    DOI： 10.4265/bio.14.161

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  • Engineering of pentose transport in Corynebacterium glutamicum to improve simultaneous utilization of mixed sugars Reviewed

    Miho Sasaki, Toru Jojima, Hideo Kawaguchi, Masayuki Inui, Hideaki Yukawa

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   85 ( 1 )   105 - 115   2009.11

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    DOI： 10.1007/s00253-009-2065-x

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  • Identification and Functional Analysis of the Gene Cluster for L-Arabinose Utilization in Corynebacterium glutamicum Reviewed

    Hideo Kawaguchi, Miho Sasaki, Alain A. Vertes, Masayuki Inui, Hideaki Yukawa

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   75 ( 11 )   3419 - 3429   2009.6

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    DOI： 10.1128/AEM.02912-08

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  • Simultaneous utilization of D-cellobiose, D-glucose, and D-xylose by recombinant Corynebacterium glutamicum under oxygen-deprived conditions Reviewed

    Miho Sasaki, Toru Jojima, Masayuki Inui, Hideaki Yukawa

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   81 ( 4 )   691 - 699   2008.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-008-1703-z

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  • Molecular cloning and characterization of cytochrome P450 and ferredoxin genes involved in bisphenol A degradation in Sphingomonas bisphenolicum strain AO1 Reviewed

    M. Sasaki, T. Tsuchido, Y. Matsumura

    JOURNAL OF APPLIED MICROBIOLOGY   105 ( 4 )   1158 - 1169   2008.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1365-2672.2008.03843.x

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  • Engineering of an L-arabinose metabolic pathway in Corynebacterium glutamicum Reviewed

    Hideo Kawaguchi, Miho Sasaki, Alain A. Vertes, Masayuki Inui, Hideaki Yukawa

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   77 ( 5 )   1053 - 1062   2008.1

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-007-1244-x

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  • Purification of cytochrome P450 and ferredoxin, involved in bisphenol A degradation, from Sphingomonas sp strain AO1 Reviewed

    M Sasaki, A Akahira, KI Oshiman, T Tsuchido, Y Matsumura

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   71 ( 12 )   8024 - 8030   2005.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1128/AEM.71.12.8024-8030.2005

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  • Biodegradation of bisphenol A by cells and cell lysate from Sphingomonas sp strain AO1 Reviewed

    M Sasaki, J Maki, K Oshiman, Y Matsumura, T Tsuchido

    BIODEGRADATION   16 ( 5 )   449 - 459   2005.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s10532-004-5023-4

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  • Purification and characterization of a Bacillus subtilis 168 nuclease, YokF, involved in chromosomal DNA degradation and cell death caused by thermal shock treatments Reviewed

    JJ Sakamoto, M Sasaki, T Tsuchido

    JOURNAL OF BIOLOGICAL CHEMISTRY   276 ( 50 )   47046 - 47051   2001.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M106205200

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Books

  • PIC/S GMPに基づく微生物学的品質管理レベルと3極局方の規格設定/試験法・バリデーション

    （ Role： Contributor）

    Science & Technology  2021.2 

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  • 菌・カビを知る知る・防ぐの60の知恵

    森美穂（ Role： Contributorpp. 6-7）

    化学同人  2015.6 

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  • Microorganisms for Xylitol Production: Focus on Strain Improvement. D-Xylitol: Fermentative Production, Application and Commercialization.

    Miho Mori（ Role： Contributor）

    2012.10 

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MISC

  • 微生物を利用した有用物質生産 1「何を原料として,何の菌を使用して,何を作るのか?」-開演にあたって- Invited

    森美穂

    日本防菌防黴学会誌   49 ( 8 )   2021

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  • 学術都市ボストンにおける大学の研究と教育 Invited

    森美穂

    化学と生物   58 ( 1 )   59 - 63   2020.1

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (scientific journal)  

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  • 防菌防黴を通じた教育と研究 Invited

    森美穂

    防菌防黴学会誌   47 ( 2 )   60   2019

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  • 乞うご期待!小さな地球の掃除屋さんの活躍 Invited

    森美穂

    生物工学会誌   96 ( 7 )   410   2018.7

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    Language：Japanese  

    J-GLOBAL

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  • 化学物質が環境と微生物に及ぼす影響 Invited

    森美穂

    アグリバイオ   2 ( 2 )   2018

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  • 高分子,内分泌撹乱物質ならびに毒性物質の生分解 Sphingomonas属細菌におけるビスフェノールA分解

    松村吉信, 佐々木美穂

    33 ( 2 )   91 - 97   2005.2

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  • Sphingomonas bisphenolica AO1のビスフェノールA分解特性

    松村吉信, 牧純一, 佐々木美穂, 大志万浩一, 土戸哲明

    日本農芸化学会誌   75   2001

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Presentations

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Awards

  • 平成25年度 日本防菌防黴学会 論文賞

    2014.5   日本防菌防黴学会  

    森美穂

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Research Projects

  • Development of efficient bioremediation technology of bisphenol A using bacteria

    Grant number：24K15346  2024.4 - 2027.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

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  • グラム陰性菌に特異的に作用する細菌由来の新規抗菌物質の探索

    Grant number：21K10408  2021.4 - 2024.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    森 美穂

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    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

    薬剤耐性菌や新たな感染症原因菌の出現は、世界的な脅威であり、有効な対策が求められている。しかしながら1960年以降、新しい作用機序を示す、副作用が小さい、薬剤耐性菌が出現しにくいなどの有用な特徴をもった抗菌物質が天然物からほとんど見つかっていない。そこで本研究では、抗菌物質生産菌が存在している可能性が高い単離源を新たに開拓することと、効率の良い生産菌の単離方法を確立し、グラム陰性菌に対して特異的に作用する新規抗菌物質の取得を目指している。単離源として、これまで用いられることが多かった土壌や環境水以外に、昆虫や植物なども対象として、グラム陰性菌に特異的に殺菌効果を示す物質生産菌の単離を試みた。また、抗菌物質は、実用化に向けた生産や服用を想定する場合、低温または高温、酸性またはアルカリ性、酵素存在下であっても安定であることなどの性質が求められるため、低温や高温、低pHや高pHの培養条件を組み合わせて、目的の細菌の単離も進めた。現時点では、昆虫から目的の菌が単離できたが、土壌や環境水を用いて培養条件を工夫した系では、目的の菌の取得に至っていない。新しい抗菌物質生産菌の単離に関する論文には、抗菌活性を示す細菌がどれだけの割合で検出されたのか、取得に至った成功のポイントなどについてはほとんど記載されていない。今後もさらにデータを蓄積し、効率よく新たな抗菌物質を取得するための単離源と単離条件を明らかにしていく予定である。

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  • ネオニコチノイド系農薬の環境における残留性と分解菌との関連性の解明

    2017.4 - 2020.3

    森美穂

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    Authorship：Principal investigator  Grant type：Competitive

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  • 家畜伝染病防除のための消石灰散布が生態系に及ぼす影響 研究課題

    2012.4 - 2015.3

    森美穂

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    Authorship：Principal investigator  Grant type：Competitive

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Other

  • しもふり豚肉の肉質特性に及ぼす長期熟成の効果

    2016.4

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    近畿大学学内研究助成金
    21世紀研究開発奨励金（共同研究助成金）
    KD13

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Teaching Experience