Updated on 2026/03/13

写真a

 
KUSAKABE Rie
 
Organization
Faculty of Chemistry, Materials and Bioengineering Associate Professor
Title
Associate Professor
Profile

広島市生まれ。関西大学化学生命工学部准教授。京都大学理学部、カリフォルニア大学バークレー校にて学部時代を過ごし、北海道大学大学院理学研究科博士課程修了、博士（理学）。専門は動物の発生生物学・進化発生学。主な実験材料はメダカで、初期胚を使った実験、遺伝子工学的な手法を使って生命の謎に迫ろうとしている。

External link

Degree

  • 博士（理学）

Research Interests

  • 脊椎動物

  • メダカ

  • Da変異体

  • 軸上筋

  • 筋分化

  • 体節

  • 軸下筋

  • ヤツメウナギ

  • microRNA

  • 形態進化

  • 脊索動物

  • 機能性RNA

Research Areas

  • Life Science / Developmental biology

  • Life Science / Evolutionary biology

Education

  • Hokkaido University

    - 2000

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    Country： Japan

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Research History

  • Kansai University   Faculty of Chemistry , Materials and Bioengineering Department of Life Science and Biotechnology   Associate Professor

    2023.4

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  • Kansai University   Faculty of Chemistry , Materials and Bioengineering

    2020.4 - 2023.3

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  • 国立研究開発法人理化学研究所   形態進化研究チーム   研究員

    2014.5 - 2023.3

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  • Kobe University   Graduate School of Science

    2013.4 - 2014.4

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  • 神戸大学 理学(系)研究科(研究院)   助教

    2010.4

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  • Kobe University   Research Scientist

    2007.11 - 2010.3

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  • ?2003年３月 日本学術振興会特別研究員(PD)、2003年４月?独立行政法人理化学研究所研究員

    2000.4

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Professional Memberships

Papers

  • Description of the embryonic development in the convict cichlid (Amatitlania nigrofasciata)

    Sayaka Matsuo, Rie Kusakabe, Shun Satoh, Kota Kambe, Kazuya Fukuda

    2026.3

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  • Embryonic and larval development of the Pacific saury Cololabis saira: Distinctive characteristics of a rapidly growing beloniform fish

    Rie Kusakabe, Shinya Yamauchi, Shigehiro Kuraku

    2026.2

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  • 円口類ヤツメウナギの遺伝子から探る脊椎動物の進化

    日下部りえ

    遺伝子医学   47   107 - 113   2024.1

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  • Thyroid and endostyle development in cyclostomes provides new insights into the evolutionary history of vertebrates Reviewed

    Wataru Takagi, Fumiaki Sugahara, Shinnosuke Higuchi, Rie Kusakabe, Juan Pascual-Anaya, Iori Sato, Yasuhiro Oisi, Nobuhiro Ogawa, Hiroshi Miyanishi, Noritaka Adachi, Susumu Hyodo, Shigeru Kuratani

    BMC Biology   20 ( 1 )   2022.12

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    Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Background

    The endostyle is an epithelial exocrine gland found in non-vertebrate chordates (amphioxi and tunicates) and the larvae of modern lampreys. It is generally considered to be an evolutionary precursor of the thyroid gland of vertebrates. Transformation of the endostyle into the thyroid gland during the metamorphosis of lampreys is thus deemed to be a recapitulation of a past event in vertebrate evolution. In 1906, Stockard reported that the thyroid gland in hagfish, the sister cyclostome group of lampreys, develops through an endostyle-like primordium, strongly supporting the plesiomorphy of the lamprey endostyle. However, the findings in hagfish thyroid development were solely based on this single study, and these have not been confirmed by modern molecular, genetic, and morphological data pertaining to hagfish thyroid development over the last century.

    Results

    Here, we showed that the thyroid gland of hagfish undergoes direct development from the ventrorostral pharyngeal endoderm, where the previously described endostyle-like primordium was not found. The developmental pattern of the hagfish thyroid, including histological features and regulatory gene expression profiles, closely resembles that found in modern jawed vertebrates (gnathostomes). Meanwhile, as opposed to gnathostomes but similar to non-vertebrate chordates, lamprey and hagfish share a broad expression domain of Nkx2-1/2-4, a key regulatory gene, in the pharyngeal epithelium during early developmental stages.

    Conclusions

    Based on the direct development of the thyroid gland both in hagfish and gnathostomes, and the shared expression profile of thyroid-related transcription factors in the cyclostomes, we challenge the plesiomorphic status of the lamprey endostyle and propose an alternative hypothesis where the lamprey endostyle could be obtained secondarily in crown lampreys.

    DOI： 10.1186/s12915-022-01282-7

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    Other Link： https://link.springer.com/article/10.1186/s12915-022-01282-7/fulltext.html

  • Developmental Evolution of Hypaxial Muscles: Insights From Cyclostomes and Chondrichthyans Reviewed

    Rie Kusakabe, Masako Tanaka, Shigeru Kuratani

    Frontiers in Cell and Developmental Biology   9   2021.9

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    Language：English   Publisher：Frontiers Media S.A.  

    DOI： 10.3389/fcell.2021.760366

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  • Developmental fates of shark head cavities reveal mesodermal contributions to tendon progenitor cells in extraocular muscles Reviewed

    Shunya Kuroda, Noritaka Adachi, Rie Kusakabe, Shigeru Kuratani

    Zoological Letters   2021.2

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    <jats:title>Abstract</jats:title><jats:p>Vertebrate extraocular muscles (EOMs) function in eye movements. The EOMs of modern jawed vertebrates consist primarily of four recti and two oblique muscles innervated by three cranial nerves. The developmental mechanisms underlying the establishment of this complex and the evolutionarily conserved pattern of EOMs are unknown. Chondrichthyan early embryos develop three pairs of overt epithelial coeloms called head cavities (HCs) in the head mesoderm, and each HC is believed to differentiate into a discrete subset of EOMs. However, no direct evidence of these cell fates has been provided due to the technical difficulty of lineage tracing experiments in chondrichthyans. Here, we set up an <jats:italic>in ovo</jats:italic> manipulation system for embryos of the cloudy catshark <jats:italic>Scyliorhinus torazame</jats:italic> and labeled the epithelial cells of each HC with lipophilic fluorescent dyes. This experimental system allowed us to trace the cell lineage of EOMs with the highest degree of detail and reproducibility to date. We confirmed that the HCs are indeed primordia of EOMs but showed that the morphological pattern of shark EOMs is not solely dependent on the early pattern of the head mesoderm, which transiently appears as tripartite HCs along the simple anteroposterior axis. Moreover, we found that one of the HCs gives rise to tendon progenitor cells of the EOMs, which is an exceptional condition in our previous understanding of head muscles; the tendons associated with head muscles have generally been supposed to be derived from cranial neural crest (CNC) cells, another source of vertebrate head mesenchyme. Based on interspecies comparisons, the developmental environment is suggested to be significantly different between the two ends of the rectus muscles, and this difference is suggested to be evolutionarily conserved in jawed vertebrates. We propose that the mesenchymal interface (head mesoderm vs CNC) in the environment of developing EOM is required to determine the processes of the proximodistal axis of rectus components of EOMs.</jats:p>

    DOI： 10.1186/s40851-021-00170-2

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  • Novel developmental bases for the evolution of hypobranchial muscles in vertebrates. Reviewed International journal

    Rie Kusakabe, Shinnosuke Higuchi, Masako Tanaka, Mitsutaka Kadota, Osamu Nishimura, Shigeru Kuratani

    BMC biology   18 ( 1 )   120 - 120   2020.9

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    BACKGROUND: Vertebrates are characterized by possession of hypobranchial muscles (HBMs). Cyclostomes, or modern jawless vertebrates, possess a rudimentary and superficial HBM lateral to the pharynx, whereas the HBM in jawed vertebrates is internalized and anteroposteriorly specified. Precursor cells of the HBM, marked by expression of Lbx1, originate from somites and undergo extensive migration before becoming innervated by the hypoglossal nerve. How the complex form of HBM arose in evolution is relevant to the establishment of the vertebrate body plan, but despite having long been assumed to be similar to that of limb muscles, modification of developmental mechanisms of HBM remains enigmatic. RESULTS: Here we characterize the expression of Lbx genes in lamprey and hagfish (cyclostomes) and catshark (gnathostome; jawed vertebrates). We show that the expression patterns of the single cyclostome Lbx homologue, Lbx-A, do not resemble the somitic expression of mammalian Lbx1. Disruption of Lbx-A revealed that LjLbx-A is required for the formation of both HBM and body wall muscles, likely due to the insufficient extension of precursor cells rather than to hindered muscle differentiation. Both homologues of Lbx in the catshark were expressed in the somitic muscle primordia, unlike in amniotes. During catshark embryogenesis, Lbx2 is expressed in the caudal HBM as well as in the abdominal rectus muscle, similar to lamprey Lbx-A, whereas Lbx1 marks the rostral HBM and pectoral fin muscle. CONCLUSIONS: We conclude that the vertebrate HBM primarily emerged as a specialized somatic muscle to cover the pharynx, and the anterior internalized HBM of the gnathostomes is likely a novelty added rostral to the cyclostome-like HBM, for which duplication and functionalization of Lbx genes would have been a prerequisite.

    DOI： 10.1186/s12915-020-00851-y

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  • Developmental mechanisms of migratory muscle precursors in medaka pectoral fin formation. Reviewed

    Tani-Matsuhana S, Kusakabe R, Inoue K

    Development genes and evolution   2018.7

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    DOI： 10.1007/s00427-018-0616-9

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  • The neural crest and evolution of the head/trunk interface in vertebrates Reviewed

    Shigeru Kuratani, Rie Kusakabe, Tatsuya Hirasawa

    Developmental Biology   2018

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Inc.  

    DOI： 10.1016/j.ydbio.2018.01.017

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  • Migratory appendicular muscles precursor cells in the common ancestor to all vertebrates. Reviewed International journal

    Eri Okamoto, Rie Kusakabe, Shigehiro Kuraku, Susumu Hyodo, Alexandre Robert-Moreno, Koh Onimaru, James Sharpe, Shigeru Kuratani, Mikiko Tanaka

    Nature ecology & evolution   1 ( 11 )   1731 - 1736   2017.11

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    In amniote embryos, skeletal muscles in the trunk are derived from epithelial dermomyotomes, the ventral margin of which extends ventrally to form body wall muscles. At limb levels, ventral dermomyotomes also generate limb-muscle precursors, an Lbx1-positive cell population that originates from the dermomyotome and migrates distally into the limb bud. In elasmobranchs, however, muscles in the paired fins were believed to be formed by direct somitic extension, a developmental pattern used by the amniote body wall muscles. Here we re-examined the development of pectoral fin muscles in catsharks, Scyliorhinus, and found that chondrichthyan fin muscles are indeed formed from Lbx-positive muscle precursors. Furthermore, these precursors originate from the ventral edge of the dermomyotome, the rest of which extends towards the ventral midline to form body wall muscles. Therefore, the Lbx1-positive, de-epithelialized appendicular muscle precursors appear to have been established in the body plan before the divergence of Chondrichthyes and Osteichthyes.

    DOI： 10.1038/s41559-017-0330-4

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  • Publisher Correction: Migratory appendicular muscles precursor cells in the common ancestor to all vertebrates. Reviewed International journal

    Eri Okamoto, Rie Kusakabe, Shigehiro Kuraku, Susumu Hyodo, Alexandre Robert-Moreno, Koh Onimaru, James Sharpe, Shigeru Kuratani, Mikiko Tanaka

    Nature ecology & evolution   1 ( 11 )   1784 - 1784   2017.11

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    In Fig. 2 of this Article originally published, some erroneous lines appeared on the left side of the images in panels c, e and g. The figure should have appeared as shown below. These errors have now been corrected in all versions of the Article.

    DOI： 10.1038/s41559-017-0374-5

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  • Developmental regulation and evolution of muscle-specific microRNAs Reviewed

    Rie Kusakabe, Kunio Inoue

    SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY   47-48   9 - 16   2015.12

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  • Complete genome sequence of the mitochondrial DNA of the river lamprey, Lethenteron japonicum Reviewed

    Yuri L. Kawai, Kei Yura, Miyuki Shindo, Rie Kusakabe, Keiko Hayashi, Kenichiro Hata, Kazuhiko Nakabayashi, Kohji Okamura

    MITOCHONDRIAL DNA   26 ( 6 )   863 - 864   2015

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    DOI： 10.3109/19401736.2013.861432

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  • MiR-124 is Involved in Post-transcriptional Regulation of Polypyrimidine Tract Binding Protein 1 (PTBP1) During Neural Development in the Medaka, Oryzias latipes Reviewed

    Yumiko Kato, Rie Kusakabe, Kunio Inoue, Shin Tochinai

    ZOOLOGICAL SCIENCE   30 ( 11 )   891 - 900   2013.11

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    DOI： 10.2108/zsj.30.891

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  • Body wall development in lamprey and a new perspective on the origin of vertebrate paired fins Reviewed

    Frank J. Tulenko, David W. McCauley, Ethan L. MacKenzie, Sylvie Mazan, Shigeru Kuratani, Fumiaki Sugahara, Rie Kusakabe, Ann C. Burke

    Proceedings of the National Academy of Sciences of the United States of America   110 ( 29 )   11899 - 11904   2013.7

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    DOI： 10.1073/pnas.1304210110

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  • Characterization of the compact bicistronic microRNA precursor, miR-1/miR-133, expressed specifically in Ciona muscle tissues Reviewed

    Rie Kusakabe, Saori Tani, Koki Nishitsuji, Miyuki Shindo, Kohji Okamura, Yuki Miyamoto, Kenta Nakai, Yutaka Suzuki, Takehiro G. Kusakabe, Kunio Inoue

    GENE EXPRESSION PATTERNS   13 ( 1-2 )   43 - 50   2013.1

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    DOI： 10.1016/j.gep.2012.11.001

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  • Developmental expression and evolution of muscle-specific microRNAs conserved in vertebrates Reviewed

    Tani, Saori, Kuraku, Shigehiro, Sakamoto, Hiroshi, Inoue, Kunio, Kusakabe, Rie

    Evolution & Development   15 ( 4 )   293 - 304   2013

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/ede.12039

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  • Expression and interaction of muscle-related genes in the lamprey imply the evolutionary scenario for vertebrate skeletal muscle, in association with the acquisition of the neck and fins. Reviewed International journal

    Rie Kusakabe, Shigehiro Kuraku, Shigeru Kuratani

    Developmental biology   350 ( 1 )   217 - 27   2011.2

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    Gnathostomes (jawed vertebrates) possess skeletal muscles with unique functional and developmental features that are absent from cyclostomes-i.e., lamprey and hagfish. These gnathostome-specific traits include the epaxial and hypaxial division of myotomes, paired fin/limb muscles, shoulder girdle muscles, and the muscle associated with the tongue and the neck. Many of these muscles are derived from several rostral somites, specifically from their hypaxial myotomic domains. However, it has not been clarified how the complicated morphology of these muscles was acquired in the evolution of vertebrates. Here we describe the expression of lamprey homologs of transcription factor genes, including a myogenic regulatory factor of the Myod family (MRF), Pax3/7, Lbx, and Zic, which play important roles in the development of ep-/hypaxial somitic muscles in gnathostomes, and show that the ventral portion of lamprey somites is comparable to the ventral dermomyotome in gnathostomes. The supra- and infraoptic muscles, derived from the two anterior somites in the lamprey, are molecularly specified before their extensive invasion into the head region. Of these, the infraoptic myotomes are suggested to represent the cucullaris homologue in the lamprey based on their topographical position in the embryonic pattern. Slightly caudal myotomes in the lamprey give rise to the hypobranchial muscle, the developmental homologue of the gnathostome hypobranchial musculature. The dorsal moieties of the lamprey somites express a Zic gene, which in teleosts specifies the epaxial identities of the somites. These evidences suggest that, although the myotomes in the ancestral jawless vertebrates do not exhibit ep-/hypaxial distinction at the morphological level, their dorsoventral specification would have already been present at gene regulatory levels, prior to the cyclostome-gnathostome divergence, which may have functioned as the key innovation to establish the ep-/hypaxial distinction in gnathostomes.

    DOI： 10.1016/j.ydbio.2010.10.029

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  • Genomic organization and embryonic expression of miR-430 in medaka (Oryzias latipes): Insights into the post-transcriptional gene regulation in early development (vol 449, pg 41, 2010) Reviewed

    Saori Tani, Rie Kusakabe, Kiyoshi Naruse, Hiroshi Sakamoto, Kunio Inoue

    GENE   457 ( 1-2 )   50 - 51   2010.6

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  • Genomic organization and embryonic expression of miR-430 in medaka (Oryzias latipes): Insights into the post-transcriptional gene regulation in early development Reviewed

    Saori Tani, Rie Kusakabe, Kiyoshi Naruse, Hiroshi Sakamoto, Kunio Inoue

    GENE   449 ( 1-2 )   41 - 49   2010.1

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    DOI： 10.1016/j.gene.2009.09.005

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  • Evolution of vertebrate skeletal myogenesis: Insights from the cyclostome lamprey Reviewed

    Kusakabe, Rie, Kuraku, Shigehiro, Kuratani, Shigeru

    Developmental Biology   344 ( 1 )   2010

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    Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.ydbio.2010.05.401

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  • Evolution of vertebrate myogenesis, with special reference to the morphological variety of skeletal muscles Reviewed

    Kusakabe, Rie, Kuraku, Shigehiro, Kuratani, Shigeru, Inoue, Kunio

    Mechanisms of Development   126   2009

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    DOI： 10.1016/j.mod.2009.06.651

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  • Expression of Sox and Fibrillar Collagen Genes in Lamprey Larval Chondrogenesis With Implications for the Evolution of Vertebrate Cartilage Reviewed

    Kaoru Ohtanii, Tuoya Yao, Mari Kobayashi, Rie Kusakabe, Shigeru Kuratani, Hiroshi Wada

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION   310B ( 7 )   596 - 607   2008.11

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jez.b.21231

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  • Identification of four Engrailed genes in the Japanese lamprey, Lethenteron japonicum. Reviewed International journal

    Manami Matsuura, Hidenori Nishihara, Koh Onimaru, Nobuhiro Kokubo, Shigehiro Kuraku, Rie Kusakabe, Norihiro Okada, Shigeru Kuratani, Mikiko Tanaka

    Developmental dynamics : an official publication of the American Association of Anatomists   237 ( 6 )   1581 - 9   2008.6

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    We have isolated four homologs of Engrailed genes from the Japanese lamprey, Lethenteron japonicum, an agnathan that occupies a critical phylogenic position between cephalochordates and gnathostomes. We named these four genes LjEngrailedA, LjEngrailedB, LjEngrailedC, and LjEngrailedD. LjEngrailedA, LjEngrailedB, and LjEngrailedD share a major expression domain in the presumptive midbrain-hindbrain boundary region of the central nervous system, although their levels and timing of expression differed. On the other hand, LjEngrailedC transcripts were in the pharyngeal ectoderm and the ventral ectoderm of the body wall. In addition, LjEngrailedA was expressed in the ventral side of the epibranchial muscle precursors. LjEngrailedD transcripts were seen in the mesodermal cells of the mandibular arch and later in a group of cells responsible for the formation of the upper lip, lower lip, and velum. Our results provide clues to the evolution of these structures as well as a possible scenario for duplication events of Engrailed genes.

    DOI： 10.1002/dvdy.21552

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  • Identification and developmental expression of two Tbx1/10-related genes in the agnathan Lethenteron japonicum. Reviewed International journal

    Eva Tiecke, Manami Matsuura, Nobuhiro Kokubo, Shigehiro Kuraku, Rie Kusakabe, Shigeru Kuratani, Mikiko Tanaka

    Development genes and evolution   217 ( 10 )   691 - 7   2007.10

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    We have identified two Tbx1/10-related genes, LjTbx1/10A and LjTbx1/10B, from the Japanese river lamprey Lethenteron japonicum. We used in situ hybridization to characterize their expression pattern during embryonic development. LjTbx1/10A and LjTbx1/10B shared common expression in the pharyngeal arches and otic vesicle, although their levels and timing of expression differed markedly. LjTbx1/10A was highly expressed in the mesodermal core of pharyngeal arches and the adjacent endoderm throughout pharyngeal arch development, whereas LjTbx1/10B expression was only transiently upregulated in forming pharyngeal pouches. LjTbx1/10A transcripts first appeared at stage 25 in otic vesicles, whereas LjTbx1/10B transcripts could already be detected in the developing otic placode at stage 20. These results suggest that lamprey LjTbx1/10A and LjTbx1/10B may play distinct roles in the patterning and development of the pharyngeal apparatus. It appears that lamprey Tbx1/10 genes have undergone subfunctionalization independent from gnathostomes, with regard to both regulation and function.

    DOI： 10.1007/s00427-007-0181-0

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  • Evolutionary perspectives from development of mesodermal components in the lamprey Reviewed

    Rie Kusakabe, Shigeru Kuratani

    DEVELOPMENTAL DYNAMICS   236 ( 9 )   2410 - 2420   2007.9

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  • Hox gene expression patterns in Lethenteron japonicum embryos--insights into the evolution of the vertebrate Hox code. Reviewed International journal

    Yoko Takio, Shigehiro Kuraku, Yasunori Murakami, Massimo Pasqualetti, Filippo M Rijli, Yuichi Narita, Shigeru Kuratani, Rie Kusakabe

    Developmental biology   308 ( 2 )   606 - 20   2007.8

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    The Hox code of jawed vertebrates is characterized by the colinear and rostrocaudally nested expression of Hox genes in pharyngeal arches, hindbrain, somites, and limb/fin buds. To gain insights into the evolutionary path leading to the gnathostome Hox code, we have systematically analyzed the expression pattern of the Hox gene complement in an agnathan species, Lethenteron japonicum (Lj). We have isolated 15 LjHox genes and assigned them to paralogue groups (PG) 1-11, based on their deduced amino acid sequences. LjHox expression during development displayed gnathostome-like spatial patterns with respect to the PG numbers. Specifically, lamprey PG1-3 showed homologous expression patterns in the rostral hindbrain and pharyngeal arches to their gnathostome counterparts. Moreover, PG9-11 genes were expressed specifically in the tailbud, implying its posteriorizing activity as those in gnathostomes. We conclude that these gnathostome-like colinear spatial patterns of LjHox gene expression can be regarded as one of the features already established in the common ancestor of living vertebrates. In contrast, we did not find evidence for temporal colinearity in the onset of LjHox expression. The genomic and developmental characteristics of Hox genes from different chordate species are also compared, focusing on evolution of the complex body plan of vertebrates.

    DOI： 10.1016/j.ydbio.2007.05.009

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  • Insights into the sequential evolutionary events toward the acquisition of paired fins

    Manami Matsuura, Eva Tiecke, Rie Kusakabe, Shigeru Kuratani, Mikiko Tanaka

    ZOOLOGICAL SCIENCE   23 ( 12 )   1184 - 1185   2006.12

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  • Evolutionary embryology resurrected in Japan with a new molecular basis: Nori Satoh and the history of ascidian studies originating in Kyoto during the 20th century Reviewed

    S Kuratani, H Wada, R Kusakabe, K Agata

    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY   50 ( 5 )   451 - 454   2006

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    DOI： 10.1387/ijdb.062154sk

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  • Evolutionary embryology revived in Japan with a new molecular basis--Nori Satoh and history of ascidian studies in Kyoto in the 20th century Reviewed

    Kuratani, S., Kusakabe, R., Wada, H., Agata, K.

    Ontogenez   37 ( 6 )   2006

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  • Evolution and developmental patterning of the vertebrate skeletal muscles: Perspectives from the lamprey Reviewed

    R Kusakabe, S Kuratani

    DEVELOPMENTAL DYNAMICS   234 ( 4 )   824 - 834   2005.12

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  • Developmental fate of the mandibular mesoderm in the lamprey, Lethenteron japonicum: Comparative morphology and development of the gnathostome jaw with special reference to the nature of the trabecula cranii. Reviewed

    Kuratani S, Murakami Y, Nobusada Y, Kusakabe R, Hirano S

    Journal of experimental zoology. Part B, Molecular and developmental evolution   302 ( 5 )   458 - 468   2004.9

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    DOI： 10.1002/jez.b.21011

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  • Developmental fate of the mandibular mesoderm in the lamprey, Lethenteron japonicum: Comparative morphology and development of the gnathostome jaw with special reference to the nature of the trabecula cranii Reviewed

    S Kuratani, Y Murakami, Y Nobusada, R Kusakabe, S Hirano

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION   302B ( 5 )   458 - 468   2004.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jez.b.21011

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  • Lamprey contractile protein genes mark different populations of skeletal muscles during development

    R Kusakabe, M Takechi, S Tochinai, S Kuratani

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION   302B ( 2 )   121 - 133   2004.3

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jez.b.20009

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  • Lamprey contractile protein genes mark different populations of skeletal muscles during development. Reviewed

    Kusakabe R, Takechi M, Tochinai S, Kuratani S

    Journal of experimental zoology. Part B, Molecular and developmental evolution   302 ( 2 )   121 - 133   2004.3

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    DOI： 10.1002/jez.b.20009

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  • Origin of the vertebrate visual cycle: Genes encoding retinal photoisomerase and two putative visual cycle proteins are expressed in whole brain of a primitive chordate Reviewed

    Y Nakashima, T Kusakabe, R Kusakabe, A Terakita, Y Shichida, M Tsuda

    JOURNAL OF COMPARATIVE NEUROLOGY   460 ( 2 )   180 - 190   2003.5

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    DOI： 10.1002/cne.10645

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  • Expression of foreign genes in lamprey embryos: an approach to study evolutionary changes in gene regulation. Reviewed

    Kusakabe R, Tochinai S, Kuratani S

    Journal of experimental zoology. Part B, Molecular and developmental evolution   296 ( 1 )   87 - 97   2003.4

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    Publishing type：Research paper (scientific journal)   Publisher：1  

    DOI： 10.1002/jez.b.11

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  • Expression of foreign genes in lamprey embryos: An approach to study evolutionary changes in gene regulation Reviewed

    R Kusakabe, S Tochinai, S Kuratani

    JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION   296B ( 1 )   87 - 97   2003.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/jez.b.00011

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  • SYSTEMATIC ANALYSIS OF CIS-REGULATORY REGIONS OF CNS-SPECIFIC GENES IN THE ASCIDIAN LARVA : II. IN VIVO FUNCTIONAL ANALYSIS(Developmental Biology,Abstracts of papers presented at the 74^<th> Annual Meeting of the Zoological Society of Japan) :

    Yoshida Reiko, Niimi Takahiro, Sakurai Daisuke, Kusakabe Rie, Horie Takeo, Tsuda Motoyuki, Kusakabe Takehiro

    Zoological science   20 ( 12 )   1561 - 1561   2003

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    Language：English   Publisher：Zoological Society of Japan  

    CiNii Books

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    Other Link： http://id.nii.ac.jp/1141/00037964/

  • Gene expression profiles in tadpole larvae of Ciona intestinalis Reviewed

    T Kusakabe, R Yoshida, Kawakami, I, R Kusakabe, Y Mochizuki, L Yamada, T Shin-i, Y Kohara, N Satoh, M Tsuda, Y Satou

    DEVELOPMENTAL BIOLOGY   242 ( 2 )   188 - 203   2002.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1006/dbio.2002.0538

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  • 1D0915 Visual cycle genes in ascidians : insights into the origin of the vertebrate visual cycle

    Nakashima Y., Kusakabe T., Kusakabe R., Horie T., Iwamoto H., Terakita A., Shichida Y., Tsuda M.

    Seibutsu Butsuri   42 ( 2 )   S22   2002

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.42.S22_3

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  • Ci-opsin1, a vertebrate-type opsin gene, expressed in the larval ocellus of the ascidian Ciona intestinalis

    T Kusakabe, R Kusakabe, Kawakami, I, Y Satou, N Satoh, M Tsuda

    FEBS LETTERS   506 ( 1 )   69 - 72   2001.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/S0014-5793(01)02877-0

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  • Ci-opsin1, a vertebrate-type opsin gene, expressed in the larval ocellus of the ascidian Ciona intestinalis Reviewed

    T Kusakabe, R Kusakabe, Kawakami, I, Y Satou, N Satoh, M Tsuda

    FEBS LETTERS   506 ( 1 )   69 - 72   2001.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/S0014-5793(01)02877-0

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  • O-Glycosylation of G-protein-coupled receptor, octopus rhodopsin - Direct analysis by FAB mass spectrometry Reviewed

    M Nakagawa, T Miyamoto, R Kusakabe, S Takasaki, T Takao, Y Shichida, M Tsuda

    FEBS LETTERS   496 ( 1 )   19 - 24   2001.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/S0014-5793(01)02392-4

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  • Why vertebrate visual cell is hyperpolarized?

    Tsuda M., Kawakami I., Kusakabe R., Kusakabe T.

    Seibutsu Butsuri   41   S67   2001

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.41.S67_4

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  • Primary structure and expression patterns of Ci-RGR1, a novel GPCR of the ascidian Ciona intestinalis

    Nakashima Y., Kusakabe R., Kusakabe T., Tuda M.

    Seibutsu Butsuri   41   S69   2001

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    Language：Japanese   Publisher：The Biophysical Society of Japan General Incorporated Association  

    DOI： 10.2142/biophys.41.S69_2

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  • In vivo analysis of two striated muscle actin promoters reveals combinations of multiple regulatory modules required for skeletal and cardiac muscle-specific gene expression Reviewed

    R Kusakabe, T Kusakabe, N Suzuki

    INTERNATIONAL JOURNAL OF DEVELOPMENTAL BIOLOGY   43 ( 6 )   541 - 554   1999.9

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  • Genomic organization and evolution of actin genes in the amphioxus Branchiostoma belcheri and Branchiostoma floridae Reviewed

    R Kusakabe, N Satoh, LZ Holland, T Kusakabe

    GENE   227 ( 1 )   1 - 10   1999.2

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/S0378-1119(98)00608-8

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Books

  • Evolutionary Developmental Biology - A Reference Guide

    （ Role： Contributor）

    Springer  2020 

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  • 動物学の百科事典

    公益社団法人日本動物学会

    丸善出版  2018.9  （ ISBN:4621303090 ）

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    Total pages：800  

    ASIN

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  • 岩波 生物学辞典 第5版

    巌佐 庸, 倉谷 滋, 斎藤 成也, 塚谷 裕一

    岩波書店  2013.2  （ ISBN:9784000803144 ）

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    Total pages：2192  

    ASIN

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  • ウォルパート発生生物学

    武田洋幸, 田村宏治

    メディカルサイエンスインターナショナル  2012.10  （ ISBN:4895927164 ）

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    Total pages：672  

    ASIN

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  • Medaka : biology, management, and experimental protocols

    木下 政人

    Wiley-Blackwell  2009  （ ISBN:9780813808710 ）

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    Language：English  

    CiNii Books

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  • 動物の形態進化のメカニズム (シリーズ 21世紀の動物科学)

    倉谷 滋, 佐藤 矩行

    培風館  2007.7  （ ISBN:456308283X ）

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    Total pages：244  

    ASIN

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MISC

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Research Projects

  • 魚類ゲノムの比較解析による運動器形成機構の解明

    2025.4 - 2026.3

    公益財団法人三菱財団  自然科学研究助成  一般助成

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    Authorship：Principal investigator 

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  • 運動器の形成・維持・老化における間葉系間質細胞の機能

    2024.4 - 2025.3

    公益財団法人ノバルティス科学振興財団  第37回ノバルティス研究奨励金 

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    Authorship：Principal investigator 

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  • 脊椎動物の「首」をつくりだす遺伝子機能の解明

    Grant number：22K06243  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    日下部 りえ

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    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

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  • 鰓下筋群の複雑化をもたらした新規の遺伝子制御

    2019.4 - 2021.3

    文部科学省  科学研究費補助金（基盤研究（C)) 

    日下部 りえ

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    Authorship：Principal investigator  Grant type：Competitive

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  • 第二心臓予定領域の進化

    2016.4 - 2018.3

    文部科学省  科学研究費補助金（基盤研究(C)) 

    日下部 りえ

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）

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  • Evolutionary developmental analysis for the origin and migration of primordial germ cells

    Grant number：25440108  2013 - 2015

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Kusakabe Rie, INOUE Kunio

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\5330000 （ Direct Cost: \4100000 、 Indirect Cost：\1230000 ）

    The cyclostome lampreys, a group of vertebrates that diverged early in evolution, retain various ancestral morphological features. In order to clarify the evolutionary pathway of origin and developmental behavior of undifferentiated precursor cells, including primordial germ cells, we conducted examination of expression patterns of developmental genes and histological observation. We also attempted the new experimental techniques for lineage tracing after introduction of artificial gene constructs. Comparison with insights from other vertebrate species implied the evolutionary relationships of the migratory undifferentiated precursor cells and the establishment of complex and diverse morphology of vertebrates.

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  • Developmental mechanisms of primordial germ cells in the cyclostome lamprey

    Grant number：23770250  2011 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    KUSAKABE Rie

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

    The cyclostome lampreys are a group of vertebrates that diverged early in evolution and retain various ancestral morphological traits. In order to explore the developmental mechanisms underlying the establishment of germ cell lineage, we examined the emergence of primordial germ cells (PGCs) and the gonads in the embryonic lampreys. Genes involved in the specification of PGCs, migration of PGCs to the gonads and in the establishment of gonadal primordium were newly identified by RT-PCR. Expression of each gene was analyzed by whole mount in situ hybridization. The results showed that maternal mRNAs of several germ cell-related genes were localized in fertilized eggs of the lamprey. We also established the experimental system in which migratory cells in the early embryos can be traced with the expression of the reporter fluorescent protein driven by upstream regulatory sequence of CXCR4 gene.

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  • microRNAの多様性と脊椎動物の系統進化

    Grant number：21770257  2009 - 2010

    文部科学省  科学研究費補助金(若手研究(B))  若手研究(B)

    日下部 りえ

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）

    脊椎動物の各系統での小分子機能性RNAの重複の度合いや機能の変遷を解明するため、複数の動物でmicroRNAの同定と機能解析を行った。平成22年度はまず、尾索動物ホヤで筋肉細胞特異的に発現するmiR-1, miR-133について、発現パターンと転写制御機構を解析した。ノザンブロット実験により各成熟型miRの発現を検出したところ、1)miR-133は変態前の胚発生過程において発現するが、miR-1は発現しないこと、2)成体では、miR-133は体壁筋のみに、miR-1は体壁筋と心筋の両方に発現することが明らかになった。さらに、miR-1/miR-133は単一の一次産物として、尾芽胚の筋肉細胞の核に局在し、その発現はmiR-1の上流領域に制御されることが見出された。現在・真骨魚類メダカについても前駆体型から成熟型へのプロセシングについて解析中である。またヤツメウナギに関し、一個のmiR-1と二個のmiR-133の相同遺伝子を同定し、前駆体の二次構造を予測した。成熟型miR-133についてノザンブロット解析を行ったところ、筋肉の発生過程で発現していた。以上の知見から、1)miR-1/miR-133は脊索動物の共通祖先においてすでに、遺伝子対としてゲノム上の近傍に位置するようになり、一続きの前駆体として筋肉特異的に産生されるようになったこと、2)脊索動物の共通祖先においてこの遺伝子対...

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  • メダカ突然変異体を用いた骨格筋パターン形成機構の進化に関する研究

    Grant number：18770212  2006 - 2008

    文部科学省  科学研究費補助金(若手研究(B))  若手研究(B)

    日下部 りえ

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    Grant amount：\3700000 （ Direct Cost: \3700000 ）

    平成19年度は、骨格筋パターニング機構をメダカとヤツメ ウナギで比較する目的で、生きたヤツメウナギ胚を用いた実験を行った。まず、ヤツメウナギの側板中胚葉であると考えられる領墟をDiIでラベルし、アンモシーテス幼生期まで飼育し固定した。次に胚を凍結切片にし、筋肉をMF20抗体で染色して観察した。その結果、DiIセラベルされた側板中胚葉と体節由来の筋肉は細胞が混じり合わないことが分かった。顎口類の移動性軸下筋では体節由来の筋芽細胞が側板中胚葉の環境下で分化するが、今回の実験からはヤツメウナギではそのような細胞間相互作用が起こらないという可能性が示唆された。また、皮筋節の発生に必須であると予想されるPax3/7遺伝子に対するモリフォリノオリゴヌレオチドを合成し、受精卵に顕微注入して発生させた。モリフォリノを注入された胚の多くに、頭部が前方に伸長できないという表現型がみられた。メダカでは、筋肉特異的microRNAであるmiR-1,miR-206,miR-133に対しLNA(locked-nucleic acid-modified oligonucleotide)プローブを作製し、in situハイブリダイゼーションを行って発現領域を同定した。その結果、miR-206はメダカ胚においてすべての骨格筋に発現するのに対し、miR-1は体幹部の筋肉には発現するが、移動性軸下筋である対鰭の...

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  • メダカ突然変異体Daを用いた脊椎動物筋肉発生様式に関する研究

    2004 - 2007

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  • 脊椎動物骨格筋の背腹パターン形成機構とその進化に関する研究

    Grant number：16770174  2004 - 2005

    文部科学省  科学研究費補助金(若手研究(B))  若手研究(B)

    日下部 りえ

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    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\3100000 （ Direct Cost: \3100000 ）

    平成17年度は、前年度に確立した骨格筋可視化メダカ系統(野生型、Da変異体)を用いて、Daの原因遺伝子であるZic1の強制発現実験を行うとともに、in situハイブリダイゼーションを用いて初期発生に働く遺伝子の発現パターンを調べた。硬節(sclerotome)のマーカーであるtwistは、野生型では体節の腹側で発現が始まり背側へと発現領域を広げるが、Da変異体では次第に発現が弱まってしまい、背側で発現することはなかった。また、Shh(ソニックヘッジホッグ)カスケードの阻害剤であるシクロパミンで野生型あるいはDa胚を処理すると、筋節の発生が異常になり、水平筋中隔が欠失、すなわち軸上・軸下の境界が失われた。しかしどちらの系統においても、対鰭には正常な筋芽細胞の移動が見られた。すなわち、対鰭に移動する特殊化した軸下筋は、軸上・軸下の区別がない状態でもアイデンティティを獲得できることがわかった。軸上・軸下の区別を持たない無顎類ヤツメウナギからZic遺伝子群を含む複数の筋肉発生関連遺伝子を単離し発現パターンを解析した。ヤツメウナギZic遺伝子は前脳などの中枢神経系に強く発現しているほか、体節の背側にも発現が見られた。また顎口類において軸下筋の発生に重要なPax3遺伝子、およびPax3に制御され四肢などに移動する軸下筋のグループのマーカーとして知られるLbx1遺伝子は、ヤツメウナギの体...

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  • 円口類ヤツメウナギを用いた脊椎動物骨格筋形態の進化に関する研究

    2000 - 2007

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