Updated on 2026/03/05

写真a

 
KATAKURA Yoshio
 
Organization
Faculty of Chemistry, Materials and Bioengineering Professor
Title
Professor
Contact information
メールアドレス
Homepage
http://biocheng.life-bio.kansai-u.ac.jp/index.html
External link

Degree

  • 農学博士 ( 1990.3 )

Research Interests

  • biomass

  • engineering ethics

  • Lactic acid bacteria

  • bioethanol;biomass;solid state fermentation;breeding of yeast

  • adhesion

  • delignification

  • research ethics

  • membrane vesicle

  • high cell density culture

  • intestinal flora

  • breeding of yeast

  • solid state fermentation

Research Areas

  • Humanities & Social Sciences / Philosophy and ethics  / 技術者倫理 研究倫理

  • Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering  / 培養工学

  • Life Science / Applied microbiology

Education

  • Osaka University   Graduate School, Division of Engineering

    1982 - 1984

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  • Osaka University   Faculty of Engineering

    - 1982

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Research History

  • Kyoto Prefectural University of Medicine

    2020

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  • Shinshu University

    2017

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  • Kansai University   Faculty of Chemistry , Materials and Bioengineering   Professor

    2010.4

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  • Osaka University

    2010 - 2022

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  • Osaka University   Graduate School of Engineering   Associate Professor

    2003.5 - 2010.4

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  • Osaka City University

    2003

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  • Osaka University   School of Engineering   Research Assistant

    1993.11 - 2003.4

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  • オリエンタル酵母工業株式会社   中央研究所   研究員

    1984.4 - 1993.10

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Professional Memberships

Committee Memberships

  • 日本生物工学会培養技術研究部会   部会長  

    2020.6 - Present   

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    Committee type：Academic society

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  • 日本乳酸菌学会   会長  

    2019.7 - 2023.6   

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    Committee type：Academic society

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  • 日本医療研究開発機構   研究倫理アドバイザー  

    2019 - Present   

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    Committee type：Academic society

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  • 日本学術振興会   研究公正アドバイザー  

    2018 - Present   

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    Committee type：Academic society

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  • 日本生物工学会   関西支部長  

    2017.4 - 2019.3   

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    Committee type：Academic society

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  • 新エネルギー・産業技術総合開発機構   技術委員  

    2017 - 2019   

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    Committee type：Government

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  • 公正研究推進協会   公正研究推進協会委員会委員  

    2016 - Present   

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    Committee type：Academic society

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  • 日本学術振興会   科学研究費委員会専門委員  

    2015 - 2016   

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    Committee type：Academic society

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  • 日本生物工学会   代議員  

    2009.9 - Present   

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    Committee type：Academic society

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  • 日本乳酸菌学会   理事  

    2009.7 - 2015.6   

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    Committee type：Academic society

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Papers

  • Immunostimulatory activity of lipoteichoic acid with three fatty acid residues derived from <i>Limosilactobacillus antri</i> JCM 15950 <sup>T</sup> Reviewed

    Shino Yamasaki-Yashiki, Tsukasa Shiraishi, Mai Gyobu, Haruna Sasaki, Jun Kunisawa, Shin-ichi Yokota, Yoshio Katakura

    Applied and Environmental Microbiology   2024.9

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    Authorship：Last author   Publishing type：Research paper (scientific journal)   Publisher：American Society for Microbiology  

    ABSTRACT

    Some strains of lactic acid bacteria can regulate the host’s intestinal immune system. Bacterial cells and membrane vesicles (MVs) of Limosilactobacillus antri JCM 15950 <sup>T</sup> promote immunoglobulin A (IgA) production in murine Peyer’s patch cells via toll-like receptor (TLR) 2. This study aimed to investigate the role of lipoteichoic acid (LTA), a ligand of TLR2, in the immunostimulatory activity of these bacterial cells and their MVs. LTA extracted from bacterial cells was purified through hydrophobic interaction chromatography and then divided into fractions LTA1 and LTA2 through anion-exchange chromatography. LTA1 induced greater interleukin (IL)-6 production from macrophage-like RAW264 cells than LTA2, and the induced IL-6 production was suppressed by TLR2 neutralization using an anti-TLR2 antibody. The LTAs in both fractions contained two hexose residues in the glycolipid anchor; however, LTA1 was particularly rich in triacyl LTA. The free hydroxy groups in the glycerol phosphate (GroP) repeating units were substituted by d -alanine ( d -Ala) and α-glucose in LTA1, but only by α-glucose in LTA2. The dealanylation of LTA1 slightly suppressed IL-6 production in RAW264 cells, whereas deacylation almost completely suppressed IL-6 production. Furthermore, IL-6 production induced by dealanylated LTA1 was markedly higher than that induced by dealanylated LTA2. These results indicated that the critical moieties for the immunostimulatory activity of L. antri -derived LTA were the three fatty acid residues rather than the substitution with d -Ala in GroP. LTA was also detected in MVs, suggesting that the triacyl LTA, but not the diacyl LTA, translocated to the MVs and conferred immunostimulatory activity.

    IMPORTANCE

    Some lactic acid bacteria activate the host intestinal immune system via toll-like receptor (TLR) 2. Lipoteichoic acid (LTA) is a TLR2 ligand; however, the moieties of LTA that determine its immunostimulatory activity remain unclear because of the wide diversity of LTA partial structures. We found that Limosilactobacillus antri JCM 15950 <sup>T</sup> has three types of LTAs (triacyl, diacyl, and monoacyl LTAs). Specifically, structural analysis of the LTAs revealed that triacyl LTA plays a crucial role in immunostimulation and that the fatty acid residues are essential for the activity. The three acyl residues are characteristic of LTAs from many lactic acid bacteria, and our findings can explain the immunostimulatory mechanisms widely exhibited by lactic acid bacteria. Furthermore, the immunostimulatory activity of membrane vesicles released by L. antri JCM 15950 <sup>T</sup> is due to the transferred LTA, demonstrating a novel mechanism of membrane vesicle-mediated immunostimulation.

    DOI： 10.1128/aem.01197-24

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  • RNA-Based Anti-Inflammatory Effects of Membrane Vesicles Derived from Lactiplantibacillus plantarum Reviewed

    Shino Yamasaki-Yashiki, Fumie Kawashima, Azusa Saika, Ryota Hosomi, Jun Kunisawa, Yoshio Katakura

    Foods   13 ( 6 )   967 - 967   2024.3

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    Authorship：Last author   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

    Bacteria generally release extracellular membrane vesicles (MVs), which are nanoparticles that play important roles in bacterial–bacterial and bacterial–host communication. As probiotics, lactic acid bacteria provide diverse health benefits to their hosts. In this study, we found that the Gram-positive lactic acid bacteria Lactiplantibacillus plantarum subsp. plantarum NBRC 15891 produce high amounts of MVs (LpMVs), and that LpMVs inhibit interleukin (IL)-8 production induced by lipopolysaccharide in intestinal epithelial HT29 cells. Heat- or UV-killed bacterial cells did not exhibit anti-inflammatory effects, and there was no uptake of these bacterial cells; contrarily, LpMVs were taken up into the cytoplasm of HT29 cells. Small RNAs extracted from LpMVs also suppressed IL-8 production in HT29 cells, suggesting that RNAs in the cytoplasm of bacterial cells are encapsulated in the MVs and released from the cells, which may be delivered to HT29 cells to exert their anti-inflammatory effects. In addition, administration of LpMVs to mice with dextran sodium sulfate-induced colitis alleviated colitis-induced weight loss and colon length shortening, indicating that LpMV intake is likely to be effective in preventing or ameliorating colitis.

    DOI： 10.3390/foods13060967

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  • Analysis of the effects of specific growth rate of Lactococcus lactis MG1363 on aerobic metabolism and its application to high-density culture Reviewed

    Journal of Bioscience and Bioengineering   136 ( 2 )   129 - 135   2023

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    Authorship：Last author,　Corresponding author  

    DOI： 10.1016/j.jbiosc.2023.05.005

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  • High productivity of immunostimulatory membrane vesicles of &lt;i&gt;Limosilactobacillus antri&lt;/i&gt; using glycine Reviewed

    Shino YAMASAKI-YASHIKI, Yu SAKAMOTO, Keiko NISHIMURA, Azusa SAIKA, Takeshi ITO, Jun KUNISAWA, Yoshio KATAKURA

    Bioscience of Microbiota, Food and Health   2023

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    Authorship：Last author   Publishing type：Research paper (scientific journal)   Publisher：BMFH Press  

    DOI： 10.12938/bmfh.2023-029

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  • 乳酸菌の好気的流加培養 Invited Reviewed

    片倉啓雄, 一瀬 涼, 神原大樹

    日本乳酸菌学会誌   33(1), 12-19   2022.3

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  • Mechanisms underlying enhanced IgA production in Peyer's patch cells by membrane vesicles derived from Lactobacillus sakei. Reviewed International journal

    Yuki Miyoshi, Azusa Saika, Takahiro Nagatake, Ayu Matsunaga, Jun Kunisawa, Yoshio Katakura, Shino Yamasaki-Yashiki

    Bioscience, biotechnology, and biochemistry   85 ( 6 )   1536 - 1545   2021.5

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    Language：English   Publishing type：Research paper (scientific journal)  

    We analyzed the mechanisms underlying enhanced IgA production in the cells of Peyer's patch cells via membrane vesicles derived from Lactobacillus sakei subsp. sakei NBRC 15893. Depletion of CD11c+ cells from Peyer's patch cells suppressed the enhanced IgA production mediated by membrane vesicles. Meanwhile, the stimulation of bone-marrow-derived dendritic cells with membrane vesicles increased gene expression of inducible nitric oxide synthase, retinaldehyde dehydrogenase 2, and several inflammatory cytokines. The production of nitric oxide and interleukin (IL)-6 by membrane vesicle stimulation was induced via Toll-like receptor 2 on bone marrow-derived dendritic cells. Inhibition of inducible nitric oxide synthase and retinaldehyde dehydrogenase 2, as well as the neutralization of IL-6 in Peyer's patch cells, suppressed the enhanced IgA production by membrane vesicle stimulation. Hence, nitric oxide, retinoic acid, and IL-6 induced by membrane vesicles play crucial roles in the enhanced IgA production elicited by membrane vesicles in Peyer's patch cells.

    DOI： 10.1093/bbb/zbab065

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  • Adhesion mechanisms of <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> JCM 10602 to dietary fiber Reviewed

    Maria Taniguchi, Minori Nambu, Yoshio Katakura, Shino Yamasaki-Yashiki

    Bioscience of Microbiota, Food and Health   40 ( 1 )   59 - 64   2021

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：BMFH Press  

    DOI： 10.12938/bmfh.2020-003

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  • Suppression of lactate production by aerobic fed-batch cultures of <i>Lactococcus lactis</i> Reviewed

    Anna Sano, Misato Takatera, Mio Kawai, Ryo Ichinose, Shino Yamasaki-Yashiki, Yoshio Katakura

    Journal of Bioscience and Bioengineering   130 ( 4 )   402 - 408   2020.10

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.jbiosc.2020.06.004

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  • Suppression of lactate production in fed-batch culture of some lactic acid bacteria with sucrose as the carbon source Reviewed

    Mio Kawai, Asami Tsuchiya, Junya Ishida, Nobuo Yoda, Shino Yashiki-Yamasaki, Yoshio Katakura

    Journal of Bioscience and Bioengineering   129 ( 5 )   535 - 540   2020.5

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    DOI： 10.1016/j.jbiosc.2019.11.009

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  • Suppression of lactate production of <i>Lactobacillus reuteri</i> JCM1112 by co-feeding glycerol with glucose Reviewed

    Ryo Ichinose, Yuichi Fukuda, Shino Yamasaki-Yashiki, Yoshio Katakura

    Journal of Bioscience and Bioengineering   129 ( 1 )   110 - 115   2020.1

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    DOI： 10.1016/j.jbiosc.2019.07.004

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  • Suppression of lactate production by using sucrose as a carbon source in lactic acid bacteria Reviewed

    Mio Kawai, Risa Harada, Nobuo Yoda, Shino Yamasaki-Yashiki, Eiichiro Fukusaki, Yoshio Katakura

    Journal of Bioscience and Bioengineering   129 ( 1 )   47 - 51   2020.1

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    Authorship：Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.jbiosc.2019.06.017

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  • Lactobacillus sakei由来メンブランベシクルによるIgA産生促進メカニズムの解明

    三好 柚紀, 井谷 彩乃, 雑賀 あずさ, 長竹 貴広, 松永 安由, 國澤 純, 片倉 啓雄, 山崎 思乃

    日本生物工学会大会講演要旨集   2019年   178 - 178   2019.8

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    Language：Japanese   Publisher：(公社)日本生物工学会  

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  • Lactobacillus antriが産生するメンブランベシクルのIgA産生促進作用の解析

    佐々木 晴菜, 仲田 真穂, 前田 新一, 雑賀 あずさ, 國澤 純, 片倉 啓雄, 山崎 思乃

    日本生物工学会大会講演要旨集   2019年   178 - 178   2019.8

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    Language：Japanese   Publisher：(公社)日本生物工学会  

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  • IgA-enhancing effects of membrane vesicles derived from <i>Lactobacillus sakei</i> subsp. <i>sakei</i> NBRC15893 Reviewed

    Shino YAMASAKI-YASHIKI, Yuki MIYOSHI, Tomoya NAKAYAMA, Jun KUNISAWA, Yoshio KATAKURA

    Bioscience of Microbiota, Food and Health   38 ( 1 )   23 - 29   2019

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    Authorship：Last author   Publishing type：Research paper (scientific journal)   Publisher：BMFH Press  

    DOI： 10.12938/bmfh.18-015

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  • 技術者倫理科目の授業改善

    大来雄二, 片倉啓雄, 下村直行

    電気学会教育フロンティア研究会資料   FIE-17-032   2017

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  • Analysis of gene expression profiles of <i>Lactobacillus paracasei</i> induced by direct contact with <i>Saccharomyces cerevisiae</i> through recognition of yeast mannan. Reviewed

    S. Yamasaki-Yashiki, H. Sawada, M. Kino-oka, Y. Katakura

    Bioscience of Microbiota, Food and Health   36 ( 1 )   17 - 25   2017

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    Authorship：Last author,　Corresponding author   Language：English   Publisher：BMFH Press  

    <p>Co-culture of lactic acid bacteria (LAB) and yeast induces specific responses that are not observed in pure culture. Gene expression profiles of <i>Lactobacillus paracasei</i> ATCC 334 co-cultured with <i>Saccharomyces cerevisiae</i> IFO 0216 were analyzed by DNA microarray, and the responses induced by direct contact with the yeast cells were investigated. Coating the LAB cells with recombinant DnaK, which acts as an adhesive protein between LAB and yeast cells, enhanced the ratio of adhesion of the LAB cells to the yeast cells. The signals induced by direct contact were clarified by removal of the LAB cells unbound to the yeast cells. The genes induced by direct contact with heat-inactivated yeast cells were very similar to both those induced by the intact yeast cells and those induced by a soluble mannan. The top 20 genes upregulated by direct contact with the heat-inactivated yeast cells mainly encoded proteins related to exopolysaccharide synthesis, modification of surface proteins, and transport systems. In the case of the most upregulated gene, LSEI_0669, encoding a protein that has a region homologous to polyprenyl glycosylphosphotransferase, the expression level was upregulated 7.6-, 11.0-, and 8.8-fold by the heat-inactivated yeast cells, the intact yeast cells, and the soluble mannan, respectively, whereas it was only upregulated 1.8-fold when the non-adherent LAB cells were not removed before RNA extraction. Our results indicated that the LAB responded to direct contact with the yeast cells through recognition of mannan on the surface of the yeast.</p>

    DOI： 10.12938/bmfh.BMFH-2016-015

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  • Simple chronic colitis model using hypopigmented mice with a Hermansky–Pudlak syndrome 5 gene mutation Reviewed

    Yumi Itoh, Yasuo Nagaoka, Yoshio Katakura, Hidehisa Kawahara, Hiroshi Takemori

    Pigment Cell and Melanoma Research   29 ( 5 )   578 - 582   2016.9

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Blackwell Publishing Ltd  

    DOI： 10.1111/pcmr.12504

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  • Commitment and Well-Being of Engineers : How to Make Students Notice and Consider Invited Reviewed

    61-9, 569-574 ( 9 )   569 - 574   2016

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    Language：Japanese  

    CiNii Books

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  • 2E16 Communication between engineers and managers

    KATAKURA Yoshio

    Proceedings of Annual Conference of Japanese Society for Engineering Education   2016   334 - 335   2016

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    Language：Japanese   Publisher：Japanese Society for Engineering Education  

    DOI： 10.20549/jseeja.2016.0_334

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  • 工学における安全と倫理 Reviewed

    片倉 啓雄

    工学教育   63-5, 13-17   2015

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  • Optimal Production of a Fusion Protein Consisting of a Single-chain Variable Fragment Antibody Against a Tumor-associated Antigen and Interleukin-2 in Fed-batch Culture of Pichia pastoris Reviewed

    Sakorn Anuleejun, Tanapat Palaga, Yoshio Katakura, Motomu Kuroki, Masahide Kuroki, Suchada Chanprateep Napathorn

    ANTICANCER RESEARCH   34 ( 8 )   3925 - 3935   2014.8

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  • Feasibility Study of Ethanol Production from Food Wastes by Consolidated Continuous Solid-State Fermentation Reviewed

    Churairat Moukamnerd, Hidehisa Kawahara, Yoshio Katakura

    Journal of Sustainable Bioenergy Systems   03 ( 02 )   143 - 148   2013

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    Authorship：Last author,　Corresponding author   Publishing type：Research paper (scientific journal)   Publisher：Scientific Research Publishing, Inc.  

    DOI： 10.4236/jsbs.2013.32020

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    Other Link： http://www.scirp.org/journal/doi.aspx?DOI=10.4236/jsbs.2013.32020

  • Isolation of a novel promoter for efficient protein expression by Aspergillus oryzae in solid-state culture Reviewed

    Hiroki Bando, Hiromoto Hisada, Hiroki Ishida, Yoji Hata, Yoshio Katakura, Akihiko Kondo

    Applied Microbiology and Biotechnology   92 ( 3 )   561 - 569   2011.11

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  • Strategy for preventing bacterial contamination by adding exogenous ethanol in solid-state semi-continuous bioethanol production Reviewed

    Yoshio Katakura, Churairat Moukamnerd, Satoshi Harashima, Masahiro Kino-oka

    Journal of Bioscience and Bioengineering   111 ( 3 )   343 - 345   2011.3

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    DOI： 10.1016/j.jbiosc.2010.11.012

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  • Ethanol production from biomass by repetitive solid-state fed-batch fermentation with continuous recovery of ethanol Reviewed

    Churairat Moukamnerd, Masahiro Kino-oka, Minetaka Sugiyama, Yoshinobu Kaneko, Chuenchit Boonchird, Satoshi Harashima, Hideo Noda, Kazuaki Ninomiya, Suteaki Shioya, Yoshio Katakura

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   88 ( 1 )   87 - 94   2010.9

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00253-010-2716-y

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  • Molecular Breeding of a Super Yeast for Efficient Bioethanol Production from Waste Clothes at High-Temperature and Low-pH Condition Reviewed

    Minetaka Sugiyama, Yoshio Katakura, Yoshinobu Kaneko, Satoshi Harashima

    SEN-I GAKKAISHI   66 ( 5 )   P159 - P163   2010.5

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  • Lactic acid bacteria display on the cell surface cytosolic proteins that recognize yeast mannan Reviewed

    Yoshio Katakura, Ryosuke Sano, Takashi Hashimoto, Kazuaki Ninomiya, Suteaki Shioya

    Applied Microbiology and Biotechnology   86 ( 1 )   319 - 326   2010.3

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00253-009-2295-y

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    Other Link： http://link.springer.com/article/10.1007/s00253-009-2295-y/fulltext.html

  • Enhanced antibody production following intermediate addition based on flux analysis in mammalian cell continuous culture Reviewed

    Takeshi Omasa, Keisuke Furuichi, Tomoya Iemura, Yoshio Katakura, Michimasa Kishimoto, Ken-ichi Suga

    BIOPROCESS AND BIOSYSTEMS ENGINEERING   33 ( 1 )   117 - 125   2010.1

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    DOI： 10.1007/s00449-009-0351-8

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  • 高温耐性・酸耐性スーパー酵母による廃棄布類からのバイオエタノール生産

    杉山峰崇, 片倉啓雄, 金子嘉信, 原島俊

    繊維学会誌   66, 159-163   2010

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  • Direct immobilization of functional single-chain variable fragment antibodies (scFvs) onto a polystyrene plate by genetic fusion of a polystyrene-binding peptide (PS-tag) Reviewed

    Yoichi Kumada, Kyoko Hamasaki, Yuki Shiritani, Aya Nakagawa, Daisuke Kuroki, Takuhito Ohse, Dong Hwan Choi, Yoshio Katakura, Michimasa Kishimoto

    ANALYTICAL AND BIOANALYTICAL CHEMISTRY   395 ( 3 )   759 - 765   2009.10

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    DOI： 10.1007/s00216-009-2999-y

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  • Proteomic analysis of responses to osmotic stress in laboratory and sake-brewing strains of Saccharomyces cerevisiae Reviewed

    Takashi Hirasawa, Kazuyuki Yamada, Keisuke Nagahisa, Thai Nho Dinh, Chikara Furusawa, Yoshio Katakura, Suteaki Shioya, Hiroshi Shimizu

    PROCESS BIOCHEMISTRY   44 ( 6 )   647 - 653   2009.6

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    DOI： 10.1016/j.procbio.2009.02.004

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  • Effect of CO2 concentration on the growth and exopolysaccharide production of Bifidobacterium longum cultivated under anaerobic conditions Reviewed

    Kazuaki Ninomiya, Kazuhiro Matsuda, Takashi Kawahata, Tadashi Kanaya, Mamiko Kohno, Yoshio Katakura, Masanori Asada, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   107 ( 5 )   535 - 537   2009.5

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    DOI： 10.1016/j.jbiosc.2008.12.015

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  • 乳酸菌Lactococcus lactisのナイシン生合成経路における律速段階の解析

    仁宮 一章, 野口 智子, 片倉 啓雄, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2009   1078 - 1078   2009

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    Language：Japanese   Publisher：公益社団法人 化学工学会  

    DOI： 10.11491/scej.2009f.0.1078.0

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  • CO2要求性ビフィズス菌における13CO2を用いた代謝解析

    片倉 啓雄, 川畑 隆司, 仁宮 一章, 金谷 忠, 河野 麻美子, 浅田 雅宣, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2009   658 - 658   2009

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    Language：Japanese   Publisher：公益社団法人 化学工学会  

    DOI： 10.11491/scej.2009.0.658.0

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  • Characterization of new isolated Ralstonia eutropha strain A-04 and kinetic study of biodegradable copolyester poly(3-hydroxybutyrate-co-4-hydroxybutyrate) production Reviewed

    Suchada Chanprateep, Yoshio Katakura, Sirirat Visetkoop, Hiroshi Shimizu, Songsri Kulpreecha, Suteaki Shioya

    JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY   35 ( 11 )   1205 - 1215   2008.11

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    DOI： 10.1007/s10295-008-0427-5

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  • Biological Detoxification of Waste House Wood Hydrolysate Using Ureibacillus thermosphaericus for Bioethanol Production Reviewed

    Naoyuki Okuda, Mayumi Soneura, Kazuaki Ninomiya, Yoshio Katakura, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   106 ( 2 )   128 - 133   2008.8

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    DOI： 10.1263/jbb.106.128

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  • New strategy for enhancement of microbial viability in simulated gastric conditions based on display of starch-binding domain on cell surface Reviewed

    Shirin Tarahomjoo, Yoshio Katakura, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 5 )   503 - 507   2008.5

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    DOI： 10.1263/jbb.105.503

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  • Rational screening of antibodies and design of sandwich enzyme linked immunosorbant assay on the basis of a kinetic model Reviewed

    Dong Hwan Choi, Yoshio Katakura, Kazuaki Ninomiya, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 3 )   261 - 272   2008.3

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    DOI： 10.1263/jbb.105.261

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  • Strategies for reducing supplemental medium cost in bioethanol production from waste house wood hydrolysate by ethanologenic Escherichia coli: Inoculum size increase and Coculture with Saccharomyces cerevisiae Reviewed

    Naoyuki Okuda, Kazuaki Ninomiya, Yoshio Katakura, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 2 )   90 - 96   2008.2

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    DOI： 10.1263/jbb.105.90

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  • Expression of c-terminal repeat region of peptidoglycan hydrolase of Lactococcus lactis IL1403 in methylotrophic yeast Pichia pastoris Reviewed

    Shirin Tarahomjoo, Yoshio Katakura, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 2 )   134 - 139   2008.2

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    DOI： 10.1263/jbb.105.134

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  • Bidirectional cell-surface anchoring function of C-terminal repeat region of peptidoglycan hydrolase of Lactococcus lactis, IL1403 Reviewed

    Shirin Tarahomjoo, Yoshio Katakura, Eiichi Satoh, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   105 ( 2 )   116 - 121   2008.2

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    DOI： 10.1263/jbb.105.116

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  • 自立配向性モノクローナル抗体を用いた革新的イムノアッセイ技術の開発

    熊田 陽一, 濱崎 今日子, 尻谷 優希, 大瀬 琢人, 片倉 啓雄, 中西 一弘, 岸本 通雅

    化学工学会 研究発表講演要旨集   2008   727 - 727   2008

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    DOI： 10.11491/scej.2008f.0.727.0

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  • 基質認識ペプチドの付加によるポリ乳酸分解酵素の比活性の向上

    住谷 昌美, 菅江 理子, 仁宮 一章, 片倉 啓雄, 中島 敏明, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2008   416 - 416   2008

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    DOI： 10.11491/scej.2008.0.416.0

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  • 乳酸菌表層提示系構築のための新規アンカータンパク質の探索と評価

    高野 真, 上條 敦史, 仁宮 一章, 片倉 啓雄, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2008   415 - 415   2008

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    DOI： 10.11491/scej.2008.0.415.0

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  • 蛍光標識cellulose binding domainを用いたリグノセルロース系バイオマス前処理プロセスの評価

    辻林 宏章, 森岡 こころ, 福井 純一, 仁宮 一章, 片倉 啓雄, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2008   389 - 389   2008

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    DOI： 10.11491/scej.2008.0.389.0

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  • Continuous recovery of ethanol from solid state fermentation of biomass

    Moukamnerd C.

    2008   993 - 993   2008

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    DOI： 10.11491/scej.2008f.0.993.0

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  • 乳酸菌Lactococcus lactisのナイシン生合成経路における律速段階の解析

    野口 智子, 森脇 久美子, Cahyani A. D., 仁宮 一章, 片倉 啓雄, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2008   986 - 986   2008

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    DOI： 10.11491/scej.2008f.0.986.0

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  • Identification of target genes conferring ethanol stress tolerance to Saccharomyces cerevisiae based on DNA microarray data analysis Reviewed

    Takashi Hirasawa, Katsunori Yoshikawa, Yuki Nakakura, Keisuke Nagahisa, Chikara Furusawa, Yoshio Katakura, Hiroshi Shimizu, Suteaki Shioya

    JOURNAL OF BIOTECHNOLOGY   131 ( 1 )   34 - 44   2007.8

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    DOI： 10.1016/j.jbiotec.2007.05.010

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  • Metabolic pathway recruiting through genomic data analysis for industrial application of Saccharomyces cerevisiae Reviewed

    Suteaki Shioya, Hiroshi Shimizu, Takashi Hirasawa, Keisuke Nagahisa, Chikara Furusawa, Gaurav Pandey, Yoshio Katakura

    BIOCHEMICAL ENGINEERING JOURNAL   36 ( 1 )   28 - 37   2007.8

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    DOI： 10.1016/j.bej.2006.06.012

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  • Fed-batch coculture of Lactobacillus kefiranofaciens with Saccharomyces cerevisiae for effective production of kefiran Reviewed

    Shiori Tada, Yoshio Katakura, Kazuaki Ninomiya, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   103 ( 6 )   557 - 562   2007.6

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    DOI： 10.1263/jbb.103.557

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  • Extracting the hidden features in saline osmotic tolerance in Saccharomyces cerevisiae from DNA microarray data using the self-organizing map: biosynthesis of amino acids Reviewed

    Gaurav Pandey, Katsunori Yoshikawa, Takashi Hirasawa, Keisuke Nagahisa, Yoshio Katakura, Chikara Furusawa, Hiroshi Shimizu, Suteaki Shioya

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   75 ( 2 )   415 - 426   2007.5

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    DOI： 10.1007/s00253-007-0837-8

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  • Simulation model for predicting limit of detection and range of quantitation of competitive enzyme-linked immunosorbent assay Reviewed

    Dong Hwan Choi, Yoshio Katakura, Rieko Matsuda, Yuzuru Hayashi, Kazuaki Ninomiya, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   103 ( 5 )   427 - 431   2007.5

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    DOI： 10.1263/jbb.103.427

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  • Enhancement of 1,4-dihydroxy-2-naphthoic acid production by Propionibactetium freudenreichii ET-3 fed-batch culture Reviewed

    Keisuke Furuichi, Yoshio Katakura, Kazuaki Ninomiya, Suteaki Shioya

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   73 ( 10 )   3137 - 3143   2007.5

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    DOI： 10.1128/AEM.01307-06

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  • Microaeration enhances productivity of bioethanol from hydrolysate of waste house wood using ethanologenic Escherichia coli KO11 Reviewed

    Naoyuki Okuda, Kazuaki Ninomiya, Masako Takao, Yoshio Katakura, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   103 ( 4 )   350 - 357   2007.4

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    DOI： 10.1263/jbb.103.350

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  • Validation of a method for predicting the precision, limit of detection and range of quantitation in competitive ELISA Reviewed

    Dong Hwan Choi, Yoshio Katakura, Rieko Matsuda, Yuzuru Hayashi, Masato Hirobe, Yasuhiro Goda, Kazuaki Ninomiya, Suteaki Shioya

    ANALYTICAL SCIENCES   23 ( 2 )   215 - 218   2007.2

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  • Enhancement of ethanol production by promoting surface contact between starch granules and arming yeast in direct ethanol fermentation Reviewed

    Teik Seong Khaw, Yoshio Katakura, Kazuaki Ninomiya, Churairat Moukamnerd, Akihiko Kondo, Mitsuyoshi Ueda, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   103 ( 1 )   95 - 97   2007.1

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    DOI： 10.1263/jbb.103.95

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  • Inverse metabolic engineering by integration of multiple omics analyses

    Hiroshi Shimizu, Takashi Hirasawa, Katsunori Yoshikawa, Yuki Nakakura, Keisuke Nagahisa, Chikara Furusawa, Yoshio Katakura, Suteaki Shioya

    IFAC Proceedings Volumes (IFAC-PapersOnline)   10   19 - 24   2007

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    DOI： 10.3182/20070604-3-mx-2914.00005

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  • Maximum production of 1,4-dihydroxy-2-naphthoic acid by fed-batch and anaerobic/aerobic culture of Propionibacterium freudenreichii ET-3

    Keisuke Furuichi, Yoshio Katakura, Kazuaki Ninomiya, Suteaki Shioya

    IFAC Proceedings Volumes (IFAC-PapersOnline)   10   121 - 126   2007

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    DOI： 10.3182/20070604-3-mx-2914.00022

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  • Comparison of transcriptional responses to osmotic stresses induced by NaCl and sorbitol additions in Saccharomyces cerevisiae using DNA microarray Reviewed

    Takashi Hirasawa, Kengo Ashitani, Katsunori Yoshikawa, Keisuke Nagahisa, Chikara Furusawa, Yoshio Katakura, Hiroshi Shimizu, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   102 ( 6 )   568 - 571   2006.12

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    DOI： 10.1263/jbb.102.568

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  • Effect of flocculation on performance of arming yeast in direct ethanol fermentation Reviewed

    Khaw Teik Seong, Yoshio Katakura, Kazuaki Ninomiya, Yohei Bito, Satoshi Katahira, Akihiko Kondo, Mitsuyoshi Ueda, Suteaki Shioya

    APPLIED MICROBIOLOGY AND BIOTECHNOLOGY   73 ( 1 )   60 - 66   2006.11

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    DOI： 10.1007/s00253-006-0454-y

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  • Optimal aerobic cultivation method for 1,4-dihydroxy-2-naphthoic acid production by Propionibacterium freudenreichii ET-3 Reviewed

    Keisuke Furuichi, Atsuko Arnano, Yoshio Katakura, Kazuaki Ninomiya, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   102 ( 3 )   198 - 205   2006.9

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    DOI： 10.1263/jbb.102.198

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  • Aerobic culture of Propionibacterium freudenreichii ET-3 can increase production ratio of 1,4-dihydroxy-2-naphthoic acid to menaquinone Reviewed

    Keisuke Furuichi, Ken-ichi Hojo, Yoshio Katakura, Kazuaki Ninomiya, Suteaki Shioya

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   101 ( 6 )   464 - 470   2006.6

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    DOI： 10.1263/jbb.101.464

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  • Evaluation of performance of different surface-engineered yeast strains for direct ethanol production from raw starch Reviewed

    Teik Seong Khaw, Yoshio Katakura, Jun Koh, Akihiko Kondo, Mitsuyoshi Ueda, Suteaki Shioya

    Applied Microbiology and Biotechnology   70 ( 5 )   573 - 579   2006.5

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  • Comparative analysis of transcriptional responses to saline stress in the laboratory and brewing strains of Saccharomyces cerevisiae with DNA microarray Reviewed

    T. Hirasawa, Y. Nakakura, K. Yoshikawa, K. Ashitani, K. Nagahisa, C. Furusawa, Y. Katakura, H. Shimizu, S. Shioya

    Applied Microbiology and Biotechnology   70 ( 3 )   346 - 357   2006.4

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  • 水生植物のコンポスト化反応を再構成できる2種の微生物について

    森岡 こころ, 川上 晋平, 仁宮 一章, 片倉 啓雄, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2006   931 - 931   2006

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    DOI： 10.11491/scej.2006f.0.931.0

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  • 酵母との共培養による乳酸菌のケフィラン生産性の向上

    片倉 啓雄, 夛田 志緒里, 佐野 良介, 仁宮 一章, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2006   1005 - 1005   2006

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    DOI： 10.11491/scej.2006f.0.1005.0

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  • 2H14-1 Simulation and precision analysis in competitive ELISA based on a kinetic model

    CHOI Dong Hwan, KATAKURA Yoshio, NINOMIYA Kazuaki, MATSUDA Rieko, HAYASHI Yuzuru, SHIOYA Suteaki

    日本生物工学会大会講演要旨集   17   223 - 223   2005.9

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  • DNA microarray analysis on Saccharomyces cerevisiae under high carbon dioxide concentration in fermentation process Reviewed

    K Nagahisa, T Nakajima, K Yoshikawa, T Hirasawa, Y Katakura, C Furusawa, S Shioya, H Shimizu

    BIOTECHNOLOGY AND BIOPROCESS ENGINEERING   10 ( 5 )   451 - 461   2005.9

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  • Effect of sulfur sources on specific desulfurization activity of Rhodococcus erythropolis KA2-5-1 in exponential fed-batch culture Reviewed

    M Konishi, K Kishimoto, T Omasa, Y Katakura, S Shioya, H Ohtake

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   99 ( 3 )   259 - 263   2005.3

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  • Construction and evaluation of drug-metabolizing cell line for bioartificial liver support system Reviewed

    T Omasa, K Kim, S Hiramatsu, Y Katakura, M Kishimoto, S Enosawa, H Ohtake

    BIOTECHNOLOGY PROGRESS   21 ( 1 )   161 - 167   2005.1

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    DOI： 10.1021/bp049757a

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  • 競合ELISA法のシミュレーションと精度の予測

    片倉 啓雄, 崔 東煥, 仁宮 一章, 松田 りえ子, 林 譲, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2005   313 - 313   2005

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    DOI： 10.11491/scej.2005.0.313.0

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  • Expression and amplification of glutamine synthetase gene endows HepG2 cells with ammonia-metabolizing activity for bioartificial liver support system Reviewed

    T Omasa, M Yamanaka, N Tanimura, Y Katakura, M Kishimoto, K Suga, S Enosawa

    ENZYME AND MICROBIAL TECHNOLOGY   35 ( 6-7 )   519 - 524   2004.12

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    DOI： 10.1016/j.enzmictec.2004.08.027

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  • A practical kinetic model for efficient isolation of useful antibodies from phage display libraries Reviewed

    Y Katakura, GQ Zhuang, T Nakatani, T Furuta, T Omasa, M Kishimoto, K Suga, S Shioya

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   28 ( 4-6 )   191 - 200   2004.6

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    DOI： 10.1016/j.molcatb.2004.02.016

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  • Rapid Construction of Gene-Amplified CHO Cell Line by Gene Targeting

    Omasa Takeshi, Kajita Mihoko, Yoshikawa Tomohiro, Katakura Yoshio, Kishimoto Michimasa, Ohtake Hisao

    Asian Pacific Confederation of Chemical Engineering congress program and abstracts   2004   488 - 488   2004

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    Gene amplification has been developed as an indispensable technique for the bio-pharmaceutical production by recombinant mammalian cells. The most commonly used technique is likely dihydroforate reductase (<I>dhfr</I>) gene amplification system in Chinese hamster ovary (CHO) cell line. In this gene amplification system, high productive CHO cells can be selected by "time-consuming" stepwise increases of methotrexiate (MTX) concentration in culture medium. However, it is of great significance to shorten the time for constructing the stable and productive recombinant CHO cell line in the industrial process. In this study, we focused on a rapid construction method for gene-amplified cell line. We employed the human granulocyte-macrophage colony stimulating factor (hGM-CSF) as a model of glycoprotein and investigated the relationship among the productivity and stability of amplified gene, the location of the amplified gene and the MTX concentration. The distribution of amplified gene on the chromosome was analyzed using fluorescence <I>in situ</I> hybridization (FISH). Based on the FISH image analysis, we speculated the specific chromosome location suitable for gene amplification.

    DOI： 10.11491/apcche.2004.0.488.0

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  • Metabolic Systems Analysis at a Key Branch Point of Glutamate Production by Coryneform Bacteria

    Shimizu Hiroshi, Shirai Tomokazu, Kuzumoto Masanori, Hirasawa Takashi, Katakura Yoshio, Nagahisa Keisuke, Furusawa Chikara, Shioya Suteaki

    Asian Pacific Confederation of Chemical Engineering congress program and abstracts   2004   423 - 423   2004

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    To improve metabolic pathways for enhancement of a targeted product systematically, quantitative systems analysis in bionetworks of gene, protein, and metabolic pathways is highly desired. Quantification of effect of perturbation of genes and proteins on metabolic fluxes enables to elucidate the parameters responsible for the control of flux in metabolic pathways. In this study, mechanism of flux redistribution at a key branch point in glutamate production by coryneform bacteria, <I>Corynebacterium glutamicum</I> and <I>Corynebacterium efficiens</I> is studied. The quantitative analysis at a key branch point, namely, 2-oxoglutarate(αKG), was performed by comparison of flux distribution before and after addition of the triggering operations such as depletion of biotin and addition of Tween 40. The flux redistribution from the central pathway to glutamate synthetic pathway obviously occurred after the decrease in activity of 2-oxoglutarate dehydrogenase complex, ODHC, while activities of other enzymes at the branch point of isocitrate dehydrogenase, ICDH, and glutamate dehydrogenase, GDH, were not significantly changed. The comparative studies on flux distribution of a wild type strain of <I>C. glutamicum</I> and two genetically engineered strains which harbor a plasmid with genes encoding ICDH and GDH, respectively, also reached the same conclusion that the ODHC is the most crucial factor for the control of flux distribution at the 2-oxoglutarate branch point. The difference in glutamate production between <I>C. glutamicum</I> and <I>C. efficiens</I> is due to a result of the difference in the degree of decrease in ODHC activity. A model with kinetic parameters well explained the phenomena of flux redistribution at the 2-oxoglutarate branch point and predicted flux redistribution.

    DOI： 10.11491/apcche.2004.0.423.0

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  • Rational Design of Metabolic Pathway Improvement based on DNA Microarray Data in Yeast

    Hirasawa Takashi, Nakakura Yuki, Hui Zhao, Nagahisa Keisuke, Katakura Yoshio, Shimizu Hiroshi, Shioya Suteaki

    Asian Pacific Confederation of Chemical Engineering congress program and abstracts   2004   422 - 422   2004

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    Growth properties and gene expression profiles by using slide glass-based DNA microarray under high osmotic pressure condition (addition of NaCl) were compared between the two yeast strains, a laboratory strain and a brewing one used for Japanese rice wine (sake) brewing. Growth properties in both strains after addition of 1 M of NaCl revealed that the brewing strain was more tolerant to osmotic stress than the laboratory one. DNA microarray analysis data indicated that the genes encoding the enzymes involved in acetate, glycerol and trehalose synthetic pathways were up-regulated in both strains under the stress conditions. Moreover, genes encoding sodium ion pump and cupper ion-binding proteins were more up-regulated in brewing strain than in the laboratory strain. Up-regulation of these genes might characterize the osmotic stress-tolerance of the brewing strain. We constructed a glycerol-overproducing strain by expressing <I>GPD1</I> gene encoding glycerol-3-phosphate dehydrogenase constitutively in the laboratory strain and evaluated the osmotic stress tolerance of this strain. This improved strain showed more tolerant to osmotic stress than the parent strain, but glycerol was immediately exported outside of the cells. We are constructing further improved strain by disrupting the gene encoding glycerol exporter protein and we will evaluate again the stress tolerance of this strain.

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  • Metabolic Pathway Recruiting through Modeling of <I>Saccharomyces cerevisiae</I> based on Genomics for Industrial Application

    Shioya Suteaki, Shimizu Hiroshi, Hirasawa Takashi, Nagahisa Keisuke, Katakura Yoshio, Furusawa Chikara

    Asian Pacific Confederation of Chemical Engineering congress program and abstracts   2004   416 - 416   2004

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    In this report, present status of a project for a process development based on genomics going on in our groups is explained. Goals of the project supported by the New Energy and Industrial Technology Development Organization (NEDO) is to build object-oriented metabolic pathway models which describe metabolic flux change due to the cultivation conditions change such as from normal one to high osmotic pressure (sugar concentration), and also to apply the model for effectively designing an improved strain producing useful compounds which can play important roles for green chemistry. The model will be developed using the data of transcriptome, proteome and metabolites. In the project, the analysis system which was composed of bioreactor system for taking the on-line/off-line data of metabolic flux analysis, NMR analysis system and software system for data mining from transcriptome as well as proteome, has been set up and took data for high sodium chloride concentration as a model of high osmolarity condition. At the same time, three sub-projects have been proceeded; (1) Basic techniques for metabolic engineering in order to control the targeted pathway flux in <I>Saccharomyces cerevisiae</I> have been developed. (2) Profiling data of transcriptome and proteome have been taken at high sodium chloride concentrations. (3) As one of clustering techniques of the profiling data, SOM (self organizing map) has been extensively studied, applied to the transcriptome data obtained in this project. The project has been now on the stage of strain improvement based on the analysis of the genome information.

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  • Analysis of Adaptation of Yeast Cells to a High Osmotic Pressure Stress based on Proteomics

    Nagahisa Keisuke, Yamada Kazuyuki, Nakakura Yuki, Hirasawa Takashi, Katakura Yoshio, Shimizu Hiroshi, Shioya Suteaki

    Asian Pacific Confederation of Chemical Engineering congress program and abstracts   2004   431 - 431   2004

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    It is one serious problem that high sugar concentration at an initial phase and/or accumulation of product during cultivation causes osmotic stress to cells in fermentation process using yeast, <I>Saccharomyces cerevisiae</I>. In this study, we analyzed the protein expression of yeast using two-dimensional electrophoresis under a high osmotic pressure condition and investigated the physiological change in yeast cell. A laboratory strain FY834 and brewing one IFO2347 were grown in YPD medium. High osmotic pressure condition was prepared by adding NaCl at final concentration of 1 M in mid-log phase. Although the growth of FY834 was stopped and that of IFO2347 was decreased, the growth of both strains was recovered later. The period between addition of NaCl and recovery of growth was 4 hours for FY834 and 2 hours for IFO2347, respectively, indicating that IFO2347 was more tolerant to osmotic stress than FY834. Protein samples were extracted from cells before and after adding NaCl and applied to two-dimensional electrophoresis. Protein expression of FY834 compared to the sample before addition of NaCl was changed after 30 min from adding NaCl, while that of IFO2347 was changed after 15 min. These results suggest that IFO2347 responds more quickly to osmotic pressure than FY834. From the view point of the metabolic pathway, the expression of proteins related to glycerol synthesis were increased after addition of NaCl in both strains, suggesting that yeast strains adapt to high osmotic pressure by synthesis of glycerol. It was consistent to the results of metabolic flux analysis.

    DOI： 10.11491/apcche.2004.0.431.0

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  • Analysis of DNA Microarray Data using Self-Organizing Map and Hierarchical Clustering

    Yoshikawa Katsunori, Pandey Gaurav, Hirasawa Takashi, Katakura Yoshio, Nagahisa Keisuke, Furusawa Chikara, Shioya Suteaki, Shimizu Hiroshi

    Asian Pacific Confederation of Chemical Engineering congress program and abstracts   2004   778 - 778   2004

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    Genome-wide expression profiles in the yeast <I>Saccharomyces cerevisiae</I> responding to environmental stresses were analyzed using microarray. Two yeast strains, a laboratory strain (FY834) and a brewing one (IFO2347) which is more osmotic tolerant than FY834 strain were analyzed and the differences between the two strains in response to several environmental stresses were elucidated. Based on the analyses of DNA microarray data, the information for breeding osmosis tolerance strain was extracted. To analyze microarray data, a Self-Organizing map (SOM) was introduced to cluster gene expression data. The range of experimental errors was determined by multiple measurements of a same sample, and the effect of expected error on the clustering results of the SOM was simulated. The clusters obtained by the SOM were further clustered until the influence of experimental errors on the clustering results falls to a certain criterion. A hierarchical clustering was adopted to cluster the SOM results, and we could obtain the clustering results which cannot be further subdivided in consideration with the experimental errors. By introducing some other information such as sequences of transcription factors, gene ontology data and metabolic pathways, we found that in the obtained clusters the frequency distributions of some factors, such as genes having specific transcription factors and genes categorized to specific functions, are significantly deviate from those computed by randomized data. Moreover we identified some clusters which represent significant up-regulation or down-regulation only in the specific conditions. For example, a gene cluster in which gene expressions were up-regulated only in IFO2347 strain was identified, and which represents the difference in osmotic tolerance between these strains.

    DOI： 10.11491/apcche.2004.0.778.0

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  • A Kinetic Study of Direct Ethanol Production from Raw Starch by Various Arming Yeast Strains

    Khaw Teik-Seong, Katakura Yoshio, Koh Jun, Kondo Akihiko, Shioya Suteaki

    Asian Pacific Confederation of Chemical Engineering congress program and abstracts   2004   789 - 789   2004

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    Single step bioconversion of raw starch to ethanol has raised considerable interest in recent years. We have developed yeast strains displaying α-amylase and glucoamylase on yeast cell surface for this purpose. The advantage of this system is that the displayed enzymes can be recovered easily with the yeast after fermentation compared to the case that these amylolytic enzymes are secreted into medium. However, display of α-amylase on cell surface may reduce the apparent association rate of the enzyme to the substrate because both of them are huge molecules, resulting a lower apparent conversion rate. The recovery of the yeast cells together with the amylolytic enzymes becomes easier when a flocculating yeast strain is used as the host strain. However, flocculation of the arming yeast may again reduce the apparent conversion rate. To establish an industrial ethanol production process with a good cost performance ratio, the advantages and disadvantages of each strategy should be evaluated quantitatively. Therefore, from a viewpoint of kinetics, we compared the performance of the fermentation systems in the case that α-amylase is displayed on the cell surface or secreted in the medium, and in the cases that a flocculating or non-flocculating strain is used as the host strain. A kinetic model has been developed to simulate the direct ethanol fermentation from raw corn starch by arming yeast. The simulation results were found agree well with experimental data obtained from direct ethanol fermentation.

    DOI： 10.11491/apcche.2004.0.789.0

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  • Changes in microflora duding composting of an aquatic plant, Brazilian elodea Reviewed

    T Nishida, T Fujimura, T Omasa, Y Katakura, K Suga, S Shioya

    JOURNAL OF CHEMICAL ENGINEERING OF JAPAN   36 ( 10 )   1201 - 1205   2003.10

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  • 水生植物のコンポスト化におけるセルロースの分解

    片倉 啓雄, 平松 真一郎, 西田 武央, 塩谷 捨明

    化学工学会 研究発表講演要旨集   2003   141 - 141   2003

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    DOI： 10.11491/scej.2003f.0.141.0

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  • Construction of a fuzzy control system for a bioreactor using biomass support particles Reviewed

    M Kishimoto, M Beluso, T Omasa, Y Katakura, H Fukuda, K Suga

    JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC   17 ( 3-5 )   207 - 213   2002.6

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  • Analysis and optimization of biopanning process of phage display libraries

    Y Katakura, GQ Zhuang, S Uchida, T Furuta, T Omasa, M Kishimoto, K Suga

    BIOLOGICAL SYSTEMS ENGINEERING   830   271 - 284   2002

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  • Design of affinity selections of ligands from phage display libraries based on kinetic studies Reviewed

    Y Katakura, S Uchida, T Furuta, K Suga, S Shioya

    6TH WORLD MULTICONFERENCE ON SYSTEMICS, CYBERNETICS AND INFORMATICS, VOL VI, PROCEEDINGS   425 - 430   2002

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  • Expression and amplification of glutamine synthetase gene for constructing ammonia-metabolizing cell lines in hybrid bioartificial liver support system Reviewed

    T Omasa, K Kim, S Hiramatsu, Y Katakura, N Kishimoto, T Matsumura, S Enosawa, S Suzuki, H Amemiya, K Suga

    ANIMAL CELL TECHNOLOGY : BASIC & APPLIED ASPECTS, VOL 12   263 - 267   2002

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  • Measurement of association rate constant of antibody-antigen interaction in solution based on enzyme-linked immunosorbent assay Reviewed

    GQ Zhuang, Y Katakura, T Omasa, M Kishimoto, KI Suga

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   92 ( 4 )   330 - 336   2001.10

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  • Flow cytometry: An improved method for the selection of highly productive gene-amplified CHO cells using flow cytometry Reviewed

    T Yoshikawa, F Nakanishi, Y Ogura, D Oi, T Omasa, Y Katakura, M Kishimoto, K Suga

    BIOTECHNOLOGY AND BIOENGINEERING   74 ( 5 )   435 - 442   2001.9

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  • A kinetic model for a biopanning process considering antigen desorption and effective antigen concentration on a solid phase Reviewed

    GQ Zhuang, Y Katakura, T Furuta, T Omasa, M Kishimoto, K Suga

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   91 ( 5 )   474 - 481   2001.5

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  • Constrained optimization of L-lysine production based on metabolic flux using a mathematical programming method Reviewed

    K Tada, M Kishimoto, T Omasa, Y Katakura, KI Suga

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   91 ( 4 )   344 - 351   2001.4

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  • Construction and evaluation for the liver fuctional HepG2 cell line in hybrid bioartificial liver support system. Reviewed

    T. Omasa, K. Kim, S. Hiramatsu, Y. Katakura, M. Kishimoto, S. Enosawa, K. Suga

    Artificial Organs   25, 819   819 - 819   2001

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  • In vitro selection of hematoporphyrin binding DNA aptamers Reviewed

    A Okazawa, H Maeda, E Fukusaki, Y Katakura, A Kobayashi

    BIOORGANIC & MEDICINAL CHEMISTRY LETTERS   10 ( 23 )   2653 - 2656   2000.12

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  • L-lysine production by exponential feeding of L-threonine Reviewed

    K Tada, M Kishimoto, T Omasa, Y Katakura, KI Suga

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   90 ( 6 )   669 - 674   2000.12

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  • Control of antibody-antigen interaction using anion-induced conformational change in antigen peptide Reviewed

    Y Katakura, T Miyazaki, H Wada, T Omasa, M Kishimoto, Y Goto, K Suga

    PROTEIN ENGINEERING   13 ( 10 )   719 - 724   2000.10

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  • Amplified gene location in chromosomal DNA affected recombinant protein production and stability of amplified genes Reviewed

    Y Yoshikawa, F Nakanishi, Y Ogura, D Oi, T Omasa, Y Katakura, M Kishimoto, K Suga

    BIOTECHNOLOGY PROGRESS   16 ( 5 )   710 - 715   2000.9

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    DOI： 10.1021/bp000114e

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  • Evaluation of stable and highly productive gene amplified CHO cell line based on the location of amplified genes Reviewed

    T Yoshikawa, F Nakanishi, S Itami, D Kameoka, T Omasa, Y Katakura, M Kishimoto, K Suga

    CYTOTECHNOLOGY   33 ( 1-3 )   37 - 46   2000.7

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  • Efficient production of desulfurizing cells with the aid of expert system Reviewed

    M Kishimoto, M Inui, T Omasa, Y Katakura, K Suga, K Okumura

    BIOCHEMICAL ENGINEERING JOURNAL   5 ( 2 )   143 - 147   2000.6

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  • On-line control of feeding of medium components to attain high cell density Reviewed

    H Suzuki, M Kishimoto, Y Kamoshita, T Omasa, Y Katakura, K Suga

    BIOPROCESS ENGINEERING   22 ( 5 )   433 - 440   2000.5

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  • Increase in desulfurization activity of Rhodococcus erythropolis KA2-5-1 using ethanol feeding Reviewed

    H Yan, M Kishimoto, T Omasa, Y Katakura, K Suga, K Okumura, O Yoshikawa

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   89 ( 4 )   361 - 366   2000.4

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  • Effect of extra N-terminal residues on the stability and folding of human lysozyme expressed in Pichia pastoris Reviewed

    S Goda, K Takano, Y Yamagata, Y Katakura, G Yutani

    PROTEIN ENGINEERING   13 ( 4 )   299 - 307   2000.4

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  • Energy metabolism and antibody production based on flux analysis in hybridoma Reviewed

    T Omasa, T Iemura, K Furuichi, Y Katakura, M Kishimoto, K Suga

    ANIMAL CELL TECHNOLOGY: CHALLENGES FOR THE 21ST CENTURY   11 - 15   1999

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  • Effect of sugar composition on the heterogeneity of antibody in hybridoma cultivation Reviewed

    T Omasa, Y Kitamoto, J Tanaka, Y Katakura, M Kishimoto, K Suga

    ANIMAL CELL TECHNOLOGY: CHALLENGES FOR THE 21ST CENTURY   139 - 143   1999

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  • Evaluation of stability in the DHFR gene amplification system using fluorescence in situ hybridization Reviewed

    F Nakanishi, T Yoshikawa, S Itami, T Omasa, Y Katakura, M Kishimoto, K Suga

    ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 10   259 - 263   1999

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  • Construction of ammonia metabolizing cell line for artificial hybrid liver support system using recombinant DNA techniques Reviewed

    T Omasa, S Urayama, M Yamanaka, N Tanimura, Y Katakura, M Kishimoto, K Suga, S Enosawa, H Amemiya

    KAGAKU KOGAKU RONBUNSHU   24 ( 2 )   184 - 189   1998.3

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  • Effect of methanol concentration on the production of human beta(2)-glycoprotein I domain V by a recombinant Pichia pastoris: A simple system for the control of methanol concentration using a semiconductor Gas Sensor Reviewed

    Y Katakura, WH Zhang, GQ Zhuang, T Omasa, M Kishimoto, W Goto, KI Suga

    JOURNAL OF FERMENTATION AND BIOENGINEERING   86 ( 5 )   482 - 487   1998

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  • The importance of ionic strength as a parameter in screening peptide ligands from a phage display library Reviewed

    Y Katakura, ET Lim, S Tsujii, T Omasa, K Suga

    JOURNAL OF FERMENTATION AND BIOENGINEERING   85 ( 4 )   447 - 450   1998

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  • Reduction of lake water eutrophication caused by nitrogen and phosphorous from paddy nearby field using a circular irrigation system Reviewed

    K Suga, N Fukui, M Tatani, Y Katakura, T Omasa, M Kishimoto

    COMPUTER APPLICATIONS IN BIOTECHNOLOGY 1998   553 - 559   1998

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  • Selection and stability for a recombinant CHO cell line expressing human GM-CSF in gene amplification Reviewed

    T Omasa, S Itami, D Kameoka, Y Katakura, K Suga

    ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 9   29 - 33   1998

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  • Effect of culture conditions on glycosylation in hybridoma antibody production Reviewed

    T Omasa, J Tanaka, K Amimoto, Y Katakura, K Suga

    ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 9   35 - 39   1998

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  • Energy metabolism and antibody production based on flux analysis in hybridoma cultivation Reviewed

    T Omasa, K Furuichi, T Iemura, Y Katakura, M Kishimoto, K Suga

    COMPUTER APPLICATIONS IN BIOTECHNOLOGY 1998   451 - 454   1998

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  • 遺伝子組換えによるハイブリッド型人工肝に適したアンモニア除去細胞の構築. Reviewed

    大政健史, 浦山重雄, 山中貢, 谷村奈緒子, 片倉啓雄, 岸本通雅, 菅健一, 絵野澤伸, 雨宮浩

    化学工学論文集   24(2), 184-189   1998

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  • Fluorescence polarization study of a salt bridge between a single chain Fv and its antigen ribonuclease A Reviewed

    Y Katakura, T Kumamoto, Y Iwai, Y Kurokawa, T Omasa, KI Suga

    MOLECULAR IMMUNOLOGY   34 ( 12-13 )   887 - 890   1997.8

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  • Fluorescence polarization study of a salt bridge between a single-chain Fv and its antigen ribonuclease A Reviewed

    Y Katakura, T Kumamoto, Y Iwai, Y Kurokawa, T Omasa, K Suga

    MOLECULAR IMMUNOLOGY   34 ( 10 )   731 - 734   1997.7

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  • Structure and function of the recombinant fifth domain of human beta(2)-glycoprotein I: Effects of specific cleavage between Lys77 and Thr78 Reviewed

    Y Hagihara, K Enjyoji, T Omasa, Y Katakura, K Suga, M Igarashi, E Matsuura, H Kato, T Yoshimura, Y Goto

    JOURNAL OF BIOCHEMISTRY   121 ( 1 )   128 - 137   1997.1

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  • Analysis of antibody production based on energy metabolism in hybridoma Reviewed

    K Furuichi, T Iemura, T Omasa, Y Katakura, K Suga

    ANIMAL CELL TECHNOLOGY: BASIC & APPLIED ASPECTS, VOL 8   261 - 265   1997

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  • Fluorescence polarization study of a salt bridge between a monoclonal antibody 3A21 and its antigen ribonuclease A. Reviewed

    Y. Katakura, T. Kumamoto, Y. Iwai, Y. Kurokawa, T. Omasa, K. Suga

    Molecular Immunology   34, 731-734   1997

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  • Fed-batch culture strategy for high yield of baker’s yeast with high fermentative activity. Reviewed

    Y. Li, J. Chen, Q. Song, Y. Katakura, S. Lun

    Chinese Journal of Biotechnology   13, 160-167   1997

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  • Cloning of cDNA and characterization of anti-RNase A monoclonal antibody 3A21 Reviewed

    Y Katakura, E Kobayashi, Y Kurokawa, T Omasa, K Fujiyama, KI Suga

    JOURNAL OF FERMENTATION AND BIOENGINEERING   82 ( 3 )   312 - 314   1996

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  • Modeling of penicillin G hydrolysis to 6-APA in an immobilized enzyme-ion exchange system. Reviewed

    J. Chen, T. Omasa, Y. Katakura, S. Shioya, K. Suga

    Chinese Journal of Biotechnology   11, 343-349   1995

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  • MOLECULAR-STRUCTURE OF A YEAST GENE, PDI1, ENCODING PROTEIN DISULFIDE ISOMERASE THAT IS ESSENTIAL FOR CELL-GROWTH Reviewed

    H TACHIKAWA, T MIURA, Y KATAKURA, T MIZUNAGA

    JOURNAL OF BIOCHEMISTRY   110 ( 2 )   306 - 313   1991.8

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  • PURIFICATION AND CHARACTERIZATION OF YEAST PROTEIN DISULFIDE ISOMERASE Reviewed

    T MIZUNAGA, Y KATAKURA, T MIURA, Y MARUYAMA

    JOURNAL OF BIOCHEMISTRY   108 ( 5 )   846 - 851   1990.11

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  • THE PRESENCE OF PROTEIN DISULFIDE ISOMERASE IN THE YEAST SACCHAROMYCES-CEREVISIAE Reviewed

    Y KATAKURA, T MIZUNAGA, T MIURA, Y MARUYAMA

    AGRICULTURAL AND BIOLOGICAL CHEMISTRY   54 ( 4 )   1043 - 1044   1990.4

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  • ENZYMATIC AND NUCLEOTIDE-SEQUENCE STUDIES OF A KANAMYCIN-INACTIVATING ENZYME ENCODED BY A PLASMID FROM THERMOPHILIC BACILLI IN COMPARISON WITH THAT ENCODED BY PLASMID PUB110 Reviewed

    M MATSUMURA, Y KATAKURA, T IMANAKA, S AIBA

    JOURNAL OF BACTERIOLOGY   160 ( 1 )   413 - 420   1984

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Books

  • 生産コストの計算

    片倉啓雄

    バイオリアクターのスケールアップと物質生産事例集. 技術情報協会  2021.4 

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  • 副生物を抑制した流加培養

    片倉啓雄

    バイオリアクターのスケールアップと物質生産事例集. 技術情報協会  2021.4 

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  • 研究公正に関するヒヤリ・ハット集

    片倉啓雄, 岡林浩嗣, 金井康郎（ Role： Supervisor (editorial)）

    国立研究開発法人日本医療研究開発機構  2020.3 

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  • 有用微生物培養のイロハ改定増補版

    片倉啓雄, 大政健史, 長沼孝文, 小野比佐好編（ Role： Joint authorpp. 1-5, 18-87, 90, 101-106, 131, 139-167, 183-216, 222, 225-256, 265-280, 310-318, 353-354（367頁中205頁執筆, 初版に33頁加筆））

    エヌ・ティ・エス  2018 

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  • 乳酸菌と酵母との相互作用，および乳酸菌の炭水化物への接着現象の解析とプロバイオティクスへの応用

    谷口茉莉亜, 山崎(屋敷)思乃, 片倉啓雄

    酵母菌・麹菌・乳酸菌の産業応用展開, シーエムシー出版  2018 

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  • バイオ実験を安全に行うために

    片倉 啓雄（ Role： Edit第3章, pp45-77（単著）, 第4章, pp79-107（共著））

    化学同人  2018 

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  • 大学生のための「安全・安心」の基礎講座 第7講「個人にできること（Ⅰ）倫理・不正防止」 Reviewed

    片倉 啓雄

    日本国立大学協会編 Web教材  2015.4 

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  • 研究者に必要な生産コストの知識

    片倉 啓雄（ Role： Sole author）

    生物工学会誌  2015 

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  • 有用微生物培養のイロハ

    片倉啓雄, 大政健史, 長沼孝文, 小野比佐好（ Role： Joint authorpp. 1-5, 18-81, 93-98, 129-137, 153-185, 195-249, 280-288, 323-324（337頁中183頁執筆））

    エヌ・ティ・エス  2014.6 

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  • 実践・化学英語リスニング生化学編

    片倉啓雄（ Role： Joint authorPart 2, Chapter 4〜6）

    化学同人  2014.4 

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  • 実践・化学英語リスニング生化学編Part 2, Chapter 4～6

    片倉啓雄（ Role： Joint author）

    化学同人  2014.4 

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  • 大学生の学びを育む学習環境のデザイン「理工系多人数講義における効果的なグループ討論」

    片倉啓雄（pp.205-217(第11章)）

    関西大学出版部  2014.3 

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  • 生物工学系の研究・開発の安全のために

    片倉 啓雄（ Role： Sole author）

    生物工学会誌  2013 

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  • 遺伝子工学 第5章「遺伝子クローニング」

    片倉 啓雄（ Role： Editpp. 68-82）

    東京化学同人  2012.3 

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  • 乳酸菌と酵母の共存と共生

    古川 壮一, 片倉 啓雄（ Role： Joint author）

    生物工学会誌  2012 

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  • 乳酸菌と酵母の共存と共生

    古川壮一, 片倉啓雄（ Role： Joint author）

    日本生物工学会誌  2012 

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  • 共生における接着の効果

    片倉 啓雄（ Role： Sole author）

    生物工学会誌  2011 

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  • バイオ系実験安全オリエンテーション

    片倉啓雄, 山本仁（ Role： Joint author97頁中95頁執筆）

    東京化学同人  2009 

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  • セルロース系バイオエタノール製造技術集成―食糧クライシス回避のために―「前処理および糖化の評価とプロセスの最適化」

    片倉 啓雄（ Role： Editpp. 97-107（第2章））

    エヌ・ティー・エス  2009 

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  • ESP e-learning for global competency “English teaching for students in science and technology”

    片倉 啓雄（ Role： Edit）

    Universal Academy Press, Inc  2008 

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  • バイオセンシングの化学工学

    片倉 啓雄（ Role： Sole author）

    化学工学  2008 

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  • 安全倫理

    片倉啓雄, 堀田源治（ Role： Joint author）

    培風館  2008 

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  • Biotechnology Basic

    片倉啓雄, 藤山和仁, 西川美香子, 福井希一（ Role： Joint author）

    大阪大学  2006 

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  • 「世界に通用するProfessional Engineerを育てるための安全教育」報告書

    片倉 啓雄（ Role： Joint translator）

    三恵社  2006 

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  • バイオプロダクション「パン酵母生産」

    片倉 啓雄（ Role： Joint authorpp. 162-166（第9章2節））

    コロナ社  2006 

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  • 生物工学ハンドブック「工学倫理」

    片倉 啓雄（ Role： Editpp 828-832）

    コロナ社  2005 

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  • Biotechnology英会話

    片倉啓雄, 藤山和人, 西川美香子, 福井希一（ Role： Joint author）

    大阪大学  2005 

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  • 乳酸菌と酵母の共培養による有用物質生産

    片倉 啓雄（ Role： Joint author）

    生物工学会誌  2004 

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  • 抗体エンジニアリングの最前線「抗原抗体反応の実践的速度論」

    片倉 啓雄（ Role： Edit第2章, pp10-16）

    シーエムシー  2004 

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  • コンビナトリアル・バイオエンジニアリング「ファージを用いたシステム−リガンドのスクリーニングとその応用におけるkinetics」

    片倉 啓雄（ Role： Editpp 27-34）

    化学同人  2003 

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  • 生物工学実験書(改訂版)「インターネットによる情報検索」

    片倉 啓雄（ Role： Editpp449-450 (第7章3節-2)）

    培風館  2002 

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  • センシングにおけるリガンド－ターゲット相互作用の反応速度論

    片倉 啓雄（ Role： Joint author）

    ケミカルエンジニアリング  2002 

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  • ファージディスプレイによる新機能分子の創出

    片倉 啓雄（ Role： Sole author）

    化学と生物  2002 

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  • バイオプロセスシステムエンジニアリング「抗体の単離及びその応用におけるモデリング」

    片倉 啓雄（ Role： Editpp 79-88）

    シーエムシー  2002 

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  • 生物工学実験書(改訂版)「インターネットの利用」

    片倉 啓雄（ Role： Editpp 449 (第7章3節-1)）

    培風館  2002 

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  • 生物工学実験書(改訂版)「指数流加培養によるパン酵母流加培養制御」

    片倉 啓雄（ Role： Editpp377-380 (第5章11節-2)）

    培風館  2002 

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  • コンビナトリアルサイエンスの新展開「ファージライブラリーから効率よくリガンドを単離するための実践的な戦略」

    片倉 啓雄（ Role： Editpp 304-317）

    シーエムシー  2002 

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  • バイオ実験のツボ－酵素活性の測定(1)

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • バイオ実験のツボ－酵素活性の測定(2)

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • バイオ実験のツボ－核酸の電気泳動

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • バイオ実験のツボ－タンパク質の定量

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • バイオ実験のツボ－遠心分離

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • バイオ実験のツボ－試料の濃縮脱塩

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • バイオ実験のツボ－試料の保存

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • タンパク質のファージディスプレイシステム

    片倉 啓雄（ Role： Joint author）

    現代化学  2001 

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  • ファージライブラリーのアフィニティ選択理論

    片倉 啓雄（ Role： Joint author）

    Bioindustry  2001 

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  • バイオ実験のツボ－実験方法の調べ方

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • バイオ実験のツボ－核酸の抽出，精製，定量

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • バイオ実験のツボ－タンパク質の電気泳動

    片倉 啓雄（ Role： Sole author）

    化学  2001 

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  • バイオ実験のツボ－植菌(2)

    片倉 啓雄（ Role： Sole author）

    化学  2000 

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  • バイオ実験のツボ－植菌(1)

    片倉 啓雄（ Role： Sole author）

    化学  2000 

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  • バイオ実験のツボ－培地の調製

    片倉 啓雄（ Role： Sole author）

    化学  2000 

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  • バイオ実験のツボ－緩衝液の調製

    片倉 啓雄（ Role： Sole author）

    化学  2000 

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  • バイオ実験のツボ－計量,調製

    片倉 啓雄（ Role： Sole author）

    化学  2000 

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  • バイオ実験のツボ－培養

    片倉 啓雄（ Role： Sole author）

    化学  2000 

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  • ファージディスプレイシステムを用いたアフィニティリガンドの創製.

    片倉 啓雄（ Role： Sole author）

    生産と技術  1998 

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  • BEACONによる抗原抗体反応の解析.

    片倉 啓雄（ Role： Sole author）

    Bioview  1997 

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  • ペプチドの構造変化を利用したアフィニティ調節

    片倉 啓雄（ Role： Joint author）

    バイオサイエンスとインダストリー  1997 

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  • バイオ研究データブック酵母編「酵母のタンパク質分泌の翻訳後修飾の問題について」

    片倉 啓雄（ Role： Editpp 9-15）

    医学出版センター  1990 

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  • Protein disulfide isomerase

    KATAKURA,Yoshio（ Role： Sole author）

    発酵工学会誌  1990 

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MISC

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Presentations

  • 研究者倫理教育にグループワークを導入する

    片倉啓雄

    2019.9 

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    Event date： 2019.9

    Venue：大手町ファ－ストスクエアカンファレンス  

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  • 科学者の責務とWell-Being～いかにして気づかせ、考えさせるか～－グループディスカッションの実践－

    片倉啓雄

    2018.11 

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    Event date： 2018.11

    Venue：大手町ファ－ストスクエアカンファレンス  

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  • 術者倫理教育の目的は何か

    片倉啓雄

    2015.5 

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    Event date： 2015.5

    Venue：姫路商工会議所  

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  • コーヒー粕由来コーヒーマンナンの構造解析と乳化特性

    笹川貴司, 片倉啓雄, 河原秀久

    日本食品工学会第14回年次大会  2013.8 

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    Event date： 2013.8

    Venue：京都テルサ  

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  • Analysis and optimization of biopanning process of phage display libraries.

    KATAKURA,Yoshio

    2002 

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    Event date： 2002

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  • Efficient production of lactic acid bacteria by aerobic fed-batch culture

    KATAKURA,Yoshio

    2021.11 

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    Venue：オンライン（日本）  

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  • キシロースを資化するSaccharomyces cerevisiaeの非組換え育種の試み

    平松孝太郎, 頓名 洋, 今野紗希, 東原成美, 山崎思乃, 片倉啓雄

    日本生物工学会第71回大会  2019.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山大学  

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  • レアメーティングにより造成した酵母の耐熱性とエタノール耐性の評価

    高橋 葵, 海道直生, 山崎思乃, 片倉啓雄

    日本生物工学会第71回大会  2019.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山大学  

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  • Lactobacillus sakei由来メンブランベシクルによるIgA産生促進メカニズムの解明

    三好柚紀, 井谷彩乃, 雑賀あずさ, 長竹貴広, 松永安由, 國澤 純, 片倉啓雄, 山崎思乃

    日本生物工学会第71回大会  2019.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山大学  

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  • Lactobacillus antriが産生するメンブランベシクルのIgA産生促進作用の解析

    佐々木晴菜, 仲田真穂, 前田新一, 雑賀あずさ, 國澤 純, 片倉啓雄, 山崎思乃

    日本生物工学会第71回大会  2019.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山大学  

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  • 蛍光式溶存酸素センサーを使用した呼吸活性の測定法

    高寺美砂都, 一瀬 涼, 山崎思乃, 片倉啓雄

    日本生物工学会第71回大会  2019.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山大学  

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  • 乳酸菌の高密度培養のための好気的流加培養による乳酸生産の抑制

    一瀬 涼, 高寺美砂都, 山崎思乃, 片倉啓雄

    日本生物工学会第71回大会  2019.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岡山大学  

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  • 科学者のWell-beingのための志向倫理 Invited

    片倉啓雄

    新潟大学研究倫理セミナー  2019.9 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：新潟大学五十嵐キャンパス  

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  • 科学者のWell-beingのための志向倫理 Invited

    片倉啓雄

    新潟大学研究倫理セミナー  2019.9 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：新潟大学旭町キャンパス  

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  • 研究者倫理教育にグループワークを導入する Invited

    片倉啓雄

    第2回 JSPS 研究倫理セミナー  2019.9 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：大手町ファ−ストスクエアカンファレンス  

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  • 固体連続並行複発酵による古紙からのエタノール生産

    岸岡拓哉, 山崎(屋敷)思乃, 片倉啓雄

    セルラーゼ研究会  2019.8 

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    Language：Japanese   Presentation type：Poster presentation  

    Venue：佐久平  

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  • The influence of specific growth rate to lactate production on aerobic fed-batch culture of lactic acid bacteriai. International conference

    M. Kawai, A. Tsuchiya, J. Ishida, N. Yoda, S. Yashiki-Yamasaki, Y. Katakura

    The 14th International Symposium in Science and Technology  2019.8 

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    Language：English   Presentation type：Poster presentation  

    Venue：Thailand  

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  • ビフィズス菌と食物繊維の接着現象の解析

    谷口茉莉亜, 南部美乃莉, 山崎(屋敷)思乃, 片倉啓雄

    日本乳酸菌学会大会  2019.7 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岐阜市  

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  • Lactococcus lactis の好気的流加培養による乳酸生産の抑制

    佐野杏奈, 河合美桜, 土屋麻美, 石田淳也, 依田伸生, 山崎(屋敷)思乃, 片倉啓雄

    日本乳酸菌学会大会  2019.7 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岐阜市  

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  • 乳酸菌の好気的流加培養における比増殖速度が乳酸生産に与える影響

    河合美桜, 土屋麻美, 石田淳也, 依田伸生, 山崎(屋敷)思乃, 片倉啓雄

    日本乳酸菌学会大会  2019.7 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岐阜市  

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  • 乳酸菌の高密度培養のための好気的流加培養による乳酸生産の抑制

    一瀬涼, 山崎(屋敷)思乃, 片倉啓雄

    2019.5 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：松山  

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  • 乳酸菌の好気的流加培養による乳酸生産抑制

    一瀬涼, 垣本智美, 山崎(屋敷)思乃, 片倉啓雄

    日本農芸化学会大会  2019.3 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京農業大学  

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  • 志向倫理的観点からの工学教育−社会の安全・安心に貢献する− Invited

    片倉啓雄

    熊本工業高等専門学校教員研修  2019.3 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：熊本工業高等専門学校  

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  • 科学者のWell-beingのための志向倫理 Invited

    片倉啓雄

    大正大学研究倫理講習  2019.2 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：大正大学  

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  • 科学者と志向倫理 Invited

    片倉啓雄

    東京医科大学研究倫理講習  2019.2 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：東京医科大学  

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  • 科学者のWell-beingのための志向倫理 Invited

    片倉啓雄

    徳島大学研究倫理講習  2019.2 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：徳島大学  

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  • 科学に携わる者のための倫理−技術者倫理教育の視点から− Invited

    片倉啓雄

    日本生命倫理学会 第30回年次大会研究公正シンポジウム  2018.12 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：京都府立医科大学  

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  • 科学者の責務とWell-Being〜いかにして気づかせ、考えさせるか〜−グループディスカッションの実践− Invited

    片倉啓雄

    第1回 JSPS 研究倫理セミナー  2018.11 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：大手町ファ−ストスクエアカンファレンス  

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  • 研究倫理と志向倫理−単なる研究不正防止を越えて− Invited

    片倉啓雄

    日本工学教育協会 第４回ワークショップ「研究倫理」  2018.11 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：東京工業大学田町キャンパス  

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  • 科学に携わる者のための倫理〜技術者倫理教育の視点から〜 Invited

    片倉啓雄

    横浜市立大学コンプライアンスセミナー  2018.9 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：横浜市立大学  

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  • 潜性形質に注目したレアメーティングによる非遺伝子組換え酵母の育種

    海道直生, 金子嘉信, 山崎(屋敷)思乃, 片倉啓雄

    日本生物工学会第70回大会  2018.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：関西大学  

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  • グルコースリプレッションを回避する流加培養による乳酸菌の乳酸生産抑制

    河合美桜, 土屋麻美, 依田伸生, 山崎(屋敷)思乃, 片倉啓雄

    日本生物工学会第70回大会  2018.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：関西大学.  

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  • 生物工学系の研究・開発の安全のために−基本的な考え方と実践例− Invited

    片倉啓雄

    日本生物工学会大会ワークショップ  2018.9 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：関西大学  

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  • Streptococcus zooepidemicusによるヒアルロン酸生産の最適化

    小川愛未, 小野聡子, 成田琴美, 武内章, 山崎(屋敷)思乃, 片倉啓雄

    日本生物工学会第70回大会  2018.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：関西大学  

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  • スクロースを糖源とした乳酸菌培養による乳酸生産抑制

    佐野杏奈, 河合美桜, 土屋麻美, 依田伸生, 山崎(屋敷)思乃, 片倉啓雄

    日本生物工学会第70回大会  2018.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：関西大学.  

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  • Lactobacillus sakei由来メンブランベシクルを介するIgA産生促進作用の解析

    三好柚紀, 山崎(屋敷)思乃, 中山知哉, 國澤純, 片倉啓雄

    日本生物工学会第70回大会  2018.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：関西大学  

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  • 腸内細菌と食物繊維の接着現象の解析

    谷口茉莉亜, 倉光香奈, 山崎(屋敷)思乃, 片倉啓雄

    日本生物工学会第70回大会  2018.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：関西大学  

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  • 志向倫理教育の実践例―ルールを提案できる人材の育成―

    片倉啓雄

    平成30年度工学教育研究講演会  2018.8 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：名古屋工業大学科学に携わる者のための倫理−技術者倫理教育の視点から−  

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  • Enhancement of intestinal IgA production by membrane vesicles derived from Lactobacillus sakei International conference

    S. Yamasaki-Yashiki, Y. Miyoshi, J. Kunisawa, Y. Katakura

    The 13th International Symposium in Science and Technology  2018.8 

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    Language：English   Presentation type：Poster presentation  

    Venue：Taiwan  

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  • 科学に携わる者のための倫理−技術者倫理教育の視点から− Invited

    片倉啓雄

    豊田中央研究所研究倫理研修  2018.8 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：豊田中央研究所  

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  • 科学に携わる者のための倫理〜技術者倫理教育の視点から〜 Invited

    片倉啓雄

    京都府立医科大学倫理研修会  2018.7 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：京都府立医科大学  

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  • スクロースの流加による乳酸菌の乳酸生産抑制

    河合美桜, 土屋麻美, 依田伸生, 山崎(屋敷)思乃, 片倉啓雄

    日本乳酸菌学会大会  2018.7 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京農業大学  

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  • 科学に携わる者のための倫理 Invited

    片倉啓雄

    千葉市医師会医療安全講習会  2018.6 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：千葉市総合保健医療センター  

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  • 乳酸菌Lactobacillus sakeiが産生するメンブランベシクルの免疫調節作用の解析

    三好柚紀, 山崎(屋敷)思乃, 仲田真穂, 中山知哉, 國澤純, 片倉啓雄

    日本乳酸菌学会泊り込みセミナー  2018.5 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：北広島クラッセホテル  

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  • 科学に携わる者のための倫理 Invited

    片倉啓雄

    川崎医療福祉大学平成30年度コンプライアンス研修  2018.5 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：川崎医療福祉大学  

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  • 志向倫理教育の実践例 Invited

    片倉啓雄

    日本工学教育協会 第3回ワークショップ「研究倫理」  2018.2 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：金沢大学東京虎ノ門キャンパス  

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  • 科学に携わる者の倫理―技術者倫理の視点から― Invited

    片倉啓雄

    日本医療研究開発機構(AMED), 研究公正シンポジウム  2017.11 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：東京・北とぴあ  

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  • 科学に携わる者の倫理―技術者倫理の視点から― Invited

    片倉啓雄

    第31回日本耳鼻咽喉科学会専門医講習会  2017.11 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：神戸ポートピアホテル  

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  • 科学に携わる者の倫理―技術者倫理の視点から― Invited

    片倉啓雄

    第31回日本耳鼻咽喉科学会専門医講習会  2017.11 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：神戸ポートピアホテル  

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  • 技術者・研究者が知るべき倫理−あなたのWell-beingのために− Invited

    片倉啓雄

    有明工業高等専門学校  2017.10 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：有明高等専門学校  

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  • 乳酸菌培養における乳酸生産の抑制

    河合美桜, 原田里紗, 土屋麻美, 依田伸生, 山崎(屋敷)思乃, 福崎英一郎, 片倉啓雄

    日本生物工学会第69回大会  2017.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：早稲田大学  

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  • Lactobacillus reuteriのグリセリン共流加による乳酸生産の抑制

    一瀬涼, 福田雄一, 山崎(屋敷)思乃, 片倉啓雄

    日本生物工学会第69回大会  2017.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：早稲田大学  

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  • 乳酸菌と食物繊維およびムチンとの相互作用の解析

    山崎(屋敷)思乃, 倉光香奈, 谷口茉莉亜, 片倉啓雄

    日本生物工学会第69回大会  2017.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：早稲田大学  

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  • 技術者・研究者が知るべき倫理−あなたのWell-beingのために− Invited

    片倉啓雄

    信州大学研究者倫理特別講義  2017.6 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：信州大学  

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  • 受講者の価値観に刺激を与える授業方法 Invited

    片倉啓雄

    日本工学教育協会 第17回ワークショップ「技術者倫理」  2017.2 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：東京工業大学  

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  • 研究倫理をどのように教えるか−技術者倫理教育の視点から− Invited

    片倉啓雄

    日本医療研究開発機構(AMED) RIOネットワーク意見聴取会  2017.1 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：東京・大手町  

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  • 研究倫理をどのように教えるか−技術者倫理教育の視点から− Invited

    片倉啓雄

    日本医療研究開発機構(AMED) RIOネットワーク意見聴取会  2016.12 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：新大阪・CICI研修センター  

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  • Characterization and modulation of cell wall components of Lactobacillus antri for the enhanced production of intestinal IgA antibody International conference

    S. Yamasaki-Yashiki, T. Toramoto, J. Kunisawa, Y. Katakura

    5th AFSLAB International Symposium  2016.11 

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    Language：English   Presentation type：Poster presentation  

    Venue：Taiwan  

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  • Lactococcus lactisのナイシン高生産株スクリーニング系の開発

    三田麻友華, 山本理奈, 大島悟, 依田伸生, 山崎(屋敷)思乃, 片倉啓雄

    日本生物工学会第68回大会  2016.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：富山国際会議場  

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  • IgA産生を増強するLactobacillus antriの細胞壁成分の解析

    寅本拓, 山崎(屋敷)思乃, 國澤純, 片倉啓雄

    日本生物工学会第68回大会  2016.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：富山国際会議場  

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  • 技術者倫理への動機づけを高める指導法 Invited

    片倉啓雄

    日本工学教育協会 第16回ワークショップ「技術者倫理」  2015.12 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：東京工業大学大岡山キャンパス  

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  • エノキタケ子実体由来熱水可溶性接着タンパク質の同定とその機能

    楮畑智代, 山崎(屋敷)思乃, 片倉啓雄, 河原秀久, 小出芳栄

    日本生物工学会第67回大会  2015.10 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：鹿児島城山観光ホテル  

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  • 酵母の固体培養における呼吸商を指標にした細胞状態の推定

    山崎(屋敷)思乃, 上田賢佳, 真田三希, 片倉啓雄

    日本生物工学会第67回大会  2015.10 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：鹿児島城山観光ホテル  

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  • 志向倫理とキャリア教育 : 社会の安全に貢献するというgood workに気づかせる

    片倉啓雄

    平成27年度工学教育研究講演会  2015.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：九州大学  

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  • Intestinal IgA enhancement by three Lactobacilli through toll-like receptor 2 activation on dendritic cells in the Peyer's patches. International conference

    S. Yamasaki-Yashiki, A. Saika, J. Kunisawa, Y. Katakura

    The 8th Asian Conference on Lactic Acid Bacteria  2015.7 

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    Language：English   Presentation type：Poster presentation  

    Venue：Bangkok, Thailand  

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  • 技術者倫理教育の目的は何か Invited

    片倉啓雄

    日本トライボロジー学会2015年会  2015.5 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：姫路商工会議所  

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  • 技術者・研究者にとってののwell-beingとは Invited

    片倉啓雄

    日本乳酸菌学会泊り込み研究会  2015.5 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：サンライズ淡路  

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  • 技術者倫理への動機づけをはかる授業例 Invited

    片倉啓雄

    日本工学教育協会 第15回ワークショップ「技術者倫理」  2014.12 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：金沢大学東京虎ノ門キャンパス  

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  • レアメーティングによるバイオエタノール生産に適した酵母の育種

    松原 巧, 山崎(屋敷)思乃, 河原秀久, 金子嘉信, 片倉啓雄

    日本生物工学会第66回大会  2014.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：札幌国際会議場  

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  • いかにして技術者の責務とやりがいに気付かせるか Invited

    片倉啓雄

    日本電気学会全国大会シンポジウム  2014.3 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：愛媛大学  

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  • キャリアデザインと技術者倫理 Invited

    片倉啓雄

    電気学会教育フロンティア研究会  2013.12 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：関西大学  

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  • 培養時の水分が乳酸菌の増殖と代謝に及ぼす影響

    菅野勇樹, 中村惇志, 安田 智, 河原秀久, 片倉啓雄

    日本生物工学会第65回大会  2013.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：広島国際会議場  

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  • コーヒー粕からの過冷却促進物質の抽出とその機能・構造解析

    濱田 純, 笹川貴司, 片倉啓雄, 河原秀久

    環境技術学会第14回年次大会  2013.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：岐阜大学  

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  • コーヒー粕由来コーヒーマンナンの構造解析と乳化特性

    笹川貴司, 片倉啓雄, 河原秀久

    日本食品工学会第14回年次大会  2013.8 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：京都テルサ  

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  • より効果的にグループ討議を進めるための準備−メッセンジャーシステムを導入した大人数クラスでのグループ討論− Invited

    片倉啓雄

    日本工学教育協会 第14回ワークショップ「技術者倫理」  2012.11 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：東京大学  

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  • グルタミン酸添加によるUreibacillus thermosphaericusの脱リグニン活性の向上

    原田里紗, 片倉啓雄, 関野宏紀, 紀ノ岡正博

    日本生物工学会大会  2012.10 

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  • Ethanol production from biomass using consolidated continuous solid-state fermentation system. International conference

    C. Moukamnerd, Y. Katakura

    XII International Symposium on Environmental Geotechnology, Energy and Global Sustainable Development  2012.6 

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    Language：English   Presentation type：Poster presentation  

    Venue：Los Angeles  

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  • 実験系学部の安全教育 …無知の罪を知り、既知の危険を学び、未知の危険に対処する人材を育てるために Invited

    片倉啓雄

    日本鉄鋼協会第163回春季講演大会  2012.3 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：横浜国立大学  

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  • 緑豆由来の不凍タンパク質の分離とその諸性質

    中地 学, 片倉 啓雄, 河原 秀久, 荒井 直樹

    日本農芸化学会大会  2012.3 

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  • 低温馴化エノキタケ子実体由来脂溶性物質の機能評価とその構造解析

    田村 淑恵, 加藤 静香, 片倉 啓雄, 河原 秀久, 竹森 洋, 小出 芳栄

    日本農芸化学会大会  2012.3 

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  • 乳酸菌の他細胞への接着と応答

    片倉啓雄

    日本生物工学会平成23年度大会  2011.9 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：東京農工大学  

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  • 基質結合部位の除去がcellobiohydrolase Iの比活性に及ぼす影響

    片倉啓雄, 橋爪祥輝, 紀ノ岡正博

    日本生物工学会平成23年度大会  2011.9 

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    Venue：東京農工大学  

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  • Genes of Lactobacillus casei induced by adhesion to yeast. International conference

    Y. Yamamoto, H. Sawada, M. Kino-oka, Y. Katakura

    The 6th Asian Conference on Lactic Acid Bacteria  2011.8 

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    Language：English   Presentation type：Poster presentation  

    Venue：Sapporo, JAPAN  

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  • Lactic acid bacteria display on the cell surface cytosolic proteins that recognize carbohydrates including mannan, cellulose and mucin. International conference

    Y. Katakura, T. Hashimoto, A. Uematsu, M. Kino-oka

    The 6th Asian Conference on Lactic Acid Bacteria  2011.8 

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    Language：English   Presentation type：Poster presentation  

    Venue：Sapporo, JAPAN  

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  • 乳酸菌と種々の炭水化物の相互作用の解析

    植松亜弥, 田中祥之, 紀ノ岡正博, 片倉啓雄

    日本乳酸菌学会2011年度大会  2011.7 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：関西大学  

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  • 酵母との接触が引き起こす乳酸菌の遺伝子発現の解析

    片倉啓雄, 澤田浩志, 山本由佳, 紀ノ岡正博

    日本乳酸菌学会2011年度大会  2011.7 

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    Venue：関西大学  

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  • Ethanol production from biomass by consolidated continuous solid state fermentation system. International conference

    KATAKURA,Yoshio

    Asian Congress on Biotechnology 2011  2011.5 

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    Language：English   Presentation type：Poster presentation  

    Venue：Shanghai, CHINA  

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  • 植物種子由来不凍タンパク質の検索とその分離

    中地 学, 小林 大基, 片倉 啓雄, 河原 秀久

    日本農芸化学会大会  2011.3 

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  • 市販キノコの細胞壁成分中の不凍活性の検索

    河原 秀久, 入江 めぐみ, 佐藤 才治, 阪口 拓也, 片倉 啓雄, 小出 芳栄

    日本農芸化学会大会  2011.3 

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  • エノキタケ細胞壁成分キシロマンナン画分の不凍活性

    藤川 和平, 坂口 拓也, 片倉 啓雄, 河原 秀久, 小出 芳栄

    日本農芸化学会大会  2011.3 

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  • 高温・酸・エタノール耐性を持つ高発酵性酵母のゲノム育種

    長谷川大介, 横田大樹, 杉山峰崇, 金子嘉信, 片倉啓雄, Chuenchit Boonchird, 原島俊

    日本生物工学会平成22年度大会  2010.10 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：宮崎市  

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  • 乳酸菌と炭水化物の相互作用

    片倉啓雄, 橋本高志, 植松亜弥, 田中祥之, 紀ノ岡正博

    日本生物工学会平成22年度大会  2010.10 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：宮崎市  

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  • 乳酸菌と炭水化物の相互作用

    片倉啓雄, 橋本高志, 田中祥之, 植松亜弥, 紀ノ岡正博

    日本生物工学会乳酸菌・腸内細菌工学研究部会講演会  2010.5 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：帯広  

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  • 倫理的問題が生じる背景と今時の学生の気質 Invited

    片倉啓雄

    日本工学教育協会 第10回ワークショップ「技術者倫理」  2010.2 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：金沢大学東京虎ノ門キャンパス  

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  • 技術者倫理教育の目的は何か−社会の安全・安心との関連を中心に考える− Invited

    片倉啓雄

    電気学会教育フロンティア研究会  2009.12 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：福井大学．  

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  • 安全を保証される立場から保証する立場へ−安全教育と技術者倫理教育の融合− Invited

    片倉啓雄

    第３回専門職・技術者倫理ワークショップ  2009.11 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：宮崎大学  

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  • 固体連続併行復発酵によるバイオエタノール生産

    片倉啓雄, Moukamnerd Churairat, 岸本淳平, 紀ノ岡正博, 金子嘉信, 原島俊, 大道徹太郎, 平尾桂一, 野田秀夫, 仁宮一章, 塩谷捨明

    日本生物工学会平成21年度大会  2009.9 

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    Venue：名古屋大学  

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  • 乳酸菌と炭水化物の接着機構の解析

    橋本高志, 植松亜弥, 仁宮 一章, 塩谷 捨明, 紀ノ岡正博, 片倉啓雄

    日本生物工学会平成21年度大会  2009.9 

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    Venue：名古屋大学  

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  • 酵母に表層提示される糖化酵素のみかけの活性に及ぼす糖鎖修飾の影響

    片倉啓雄, 加藤真由, 仁宮一章, 近藤昭彦, 植田充美, 塩谷捨明

    日本生物工学会平成21年度大会  2009.9 

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    Venue：名古屋大学  

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  • 乳酸菌Lactococcus lactisのナイシン生合成経路における律速段階の解析

    仁宮一章, 野口智子, 片倉啓雄, 塩谷捨明

    日本生物工学会平成21年度大会  2009.9 

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    Venue：名古屋大学  

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  • 大学での安全教育−学生自身に気づかせ、考えさせ、実践させる− Invited

    片倉啓雄

    日本鉄鋼協会第157回春季講演大会  2009.3 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：東京工業大学  

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  • Ethanol production from biomass by Consolidated Continuous Solid State Fermentation System

    Moukamnerd Churairat, 原田佳苗, 大道徹太郎, 平尾桂一, 片倉啓雄, 仁宮一章, 塩谷捨明

    化学工学会第73年会  2009.3 

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    Venue：横浜国立大学  

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  • CO2要求性ビフィズス菌における13CO2を用いた代謝解析

    片倉啓雄, 川畑隆司, 仁宮一章, 金谷 忠, 河野麻美子, 浅田雅宣, 塩谷捨明

    化学工学会第73年会  2009.3 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：横浜国立大学  

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  • 乳酸菌と酵母の接着とその効果 Invited

    片倉啓雄

    日本農芸化学会2009年度大会（招待講演）  2009.3 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：福岡国際会議場．  

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  • 安全倫理−気づかせ、考えさせ、実践させる− Invited

    片倉啓雄

    日本工学教育協会 第9回ワークショップ「技術者倫理」  2009.2 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：日本大学理工学部  

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  • 安全倫理−社会の安全・安心のための基本をどのように教えるか− Invited

    片倉啓雄

    日本鉄鋼協会安全・快適なシステム構築研究フォーラム 第13回公開セミナー  2008.11 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：日本鉄鋼協会  

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  • 技術者倫理教育と安全教育との融合−技術者が知るべき実践的安全倫理−

    片倉啓雄

    平成20年度工学・工業教育研究講演会  2008.8 

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    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：神戸大学．  

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  • 安全を保証される立場から保証する立場へ Invited

    片倉啓雄

    日本工学教育協会 第8回ワークショップ「技術者倫理」  2008.2 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：日本大学理工学部  

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  • 大学の安全と社会の安全 Invited

    片倉啓雄

    青山学院大学理工学部  2007.11 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：青山学院大学相模原キャンパス  

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  • 工学倫理教育とバイオテクノロジー Invited

    片倉啓雄

    平成20年度日本機械学会大会  2007.9 

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    Language：Japanese   Presentation type：Oral presentation (invited, special)  

    Venue：関西大学．  

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  • 大学の安全と社会の安全 Invited

    片倉啓雄

    第１回大阪大学工学研究科技術部技術職員研修  2007.9 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：大阪大学．  

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  • 安全にはトップダウンとボトムアップの双方が必要−学生に能動的に安全活動に参加させるには− Invited

    片倉啓雄

    私立大学環境保全協議会第22回夏期研修研究会  2007.8 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：同志社大学  

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  • 生化学系実験の安全−事故事例とその対策− Invited

    片倉啓雄

    東京大学工学研究科安全講習  2007.6 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：東京大学工学研究科  

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  • 技術者教育における安全とは−大阪大学での実践例− Invited

    片倉啓雄

    愛媛大学農学部安全講習  2007.3 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：愛媛大学農学部．  

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  • 研究室での安全への取り組み−大阪大学での好事例を中心に− Invited

    片倉啓雄

    第2回安心と安全のための教育シンポジウム「安全・安心な教育研究環境を実現するには」  2007.1 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：大阪大学  

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  • 大阪大学工学部応用自然科学系の技術者倫理教育−気づかせ，考えさせるボトムアップ的な安全・倫理教育を目指して− Invited

    片倉啓雄

    第1回室蘭工業大学特色ＧＰシンポジウム「技術者倫理教育の現状と課題」  2006.12 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：室蘭工業大学．  

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  • 学生自身に考えさせるボトムアップ的な安全・倫理教育を目指して Invited

    片倉啓雄

    第1回安心と安全のための教育シンポジウム「教え子の安全・安心・健康への感性を高めるには」  2006.1 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：大阪大学  

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  • 工学における安全と倫理−応用生物工学専攻での授業コンテンツ Invited

    片倉啓雄

    ワークショップ「技術者倫理の構築」  2004.1 

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    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Venue：北海道大学  

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  • On rate selection of phage display antibody libraries: a rational strategy for isolating antibodies enabling rapid assay. International conference

    KATAKURA,Yoshio

    Phage display: The Chemical Set for Proteins.  2003 

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    Language：English   Presentation type：Poster presentation  

    Venue：Cambridge, Massachusetts, USA.  

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  • Design of affinity selections of ligands from phage display libraries based on kinetic studies. International conference

    KATAKURA,Yoshio

    The 6th world multiconference on systematics, cybernetics and informatics.  2002 

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    Language：English   Presentation type：Oral presentation (general)  

    Venue：Ohland, USA  

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  • Measurement of association rate constant of antibody-antigen interaction based on ELISA. Recombinant Antibody. International conference

    KATAKURA,Yoshio

    Recombinant Antibody  2002 

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    Language：English   Presentation type：Poster presentation  

    Venue：Cambridge, Massachusetts, USA.  

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  • A kinetic model for biopanning process considering antigen desorption and effective antigen concentration on solid phase. International conference

    KATAKURA,Yoshio

    Phage display technologies: Derect Protein Evolution  2001 

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    Language：English   Presentation type：Poster presentation  

    Venue：Cambridge, Massachusetts, USA.  

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  • Analysis and optimization of biopanning process of phage display libraries. International conference

    KATAKURA,Yoshio

    2000 International Chemical Congress of Pacific Basin Societies "Advance in Biochemical Production Technology"  2000 

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    Language：English   Presentation type：Poster presentation  

    Venue：Honolulu, Hawai, USA  

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  • Screening of pH-dependent antibodies from a phage display library by using affinity chromatography. International conference

    KATAKURA,Yoshio

    Recombinant Antibodies.  2000 

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    Language：English   Presentation type：Poster presentation  

    Venue：Baltimore, Martland, USA.  

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  • Fluorescence polarization study on a salt bridge between a single chain Fv and its antigen ribonuclease A. International conference

    KATAKURA,Yoshio

    Recombinant Antibodies.  2000 

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    Language：English   Presentation type：Poster presentation  

    Venue：Baltimore, Martland, USA  

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  • Energy metabolism and antibody production based on flux analysis in hybridoma. International conference

    KATAKURA,Yoshio

    Animal Cell Technology  1999 

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    Language：English  

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  • Control of an antibody-antigen interaction using an anion-induced conformational change in the antigen peptide. International conference

    KATAKURA,Yoshio

    Bioseparation Engineering: Recovery and recycle of resources to protect the global environment  1999 

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    Language：English   Presentation type：Poster presentation  

    Venue：Nikko, Tochigi  

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  • Energy metabolism and antibody production based on flux analysis in hybridoma cultivation. International conference

    KATAKURA,Yoshio

    Computer Application in Biotechnology  1998 

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    Language：English  

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  • Reduction of lake water eutrophication caused by nitrogen and phosphorous from paddy nearby field using a circular irrigation system. International conference

    KATAKURA,Yoshio

    Computer Application in Biotechnology  1998 

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    Language：English  

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Industrial property rights

  • 過冷却促進剤、過冷却促進剤の製造方法、抗凝固性組成物、及び、抗凝固性組成物の製造方法

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    Application no：2013-169606  Date applied：2013.8

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  • 界面活性剤及び界面活性剤の製造方法

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    Application no：2013-010967  Date applied：2013.1

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  • 同時糖化発酵方法

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    Application no：2012-064820  Date applied：2012.3

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  • エタノールの製造方法

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    Application no：2010-194832  Date applied：2010.8

    Announcement no：2012-50376  Date announced：2012.3

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  • 微生物複合体

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    Application no：2007-325246  Date applied：2007.12

    Announcement no：WO/2009/078438  Date announced：2009.6

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  • 蛋白質の消化物

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    Date applied：1993.6

    Announcement no：07-008179 

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  • CHO由来α1,6フコース転移酵素およびその遺伝子

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    Announcement no：2003-245079  Date announced：2003.9

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  • 糖蛋白質の生産方法

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    Announcement no：WO02/002793  Date announced：2003.8

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  • 高い会合速度定数を持つリガンドの選択法

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    Announcement no：2003-313197  Date announced：2003.1

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  • ポリマーの分子量変化の分析方法，およびその方法に用いる蛍光標識化合物

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    Announcement no：2003-247946  Date announced：2002.2

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  • 乳酸菌のスクリーニング方法

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    Application no：2016-162760 

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  • 乳酸菌の培養方法

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    Application no：2017-151959 

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  • 乳酸菌の培養方法

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    Application no：2017-151958 

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  • 乳酸菌の培養方法及び食品材料

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    Application no：2013-171447 

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  • 遺伝子増幅細胞の迅速選択法

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    Announcement no：2001-037478  Date announced：2001.3

    Patent/Registration no：4031154  Date issued：2007.12

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Works

  • 日本学術振興会 大学院生向け研究倫理eラーニングコース

    片倉啓雄, 中村征樹, 岡林浩嗣

    2019.7

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    Work type：Web Service  

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  • 大学院生向け研究倫理eラーニングコース

    片倉啓雄, 中村征樹, 岡林浩嗣

    2019

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  • 「大学生のための「安全・安心」の基礎講座」第7講「個人にできること（I）倫理・不正防止」日本国立大学協会編

    片倉啓雄

    2015

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    Work type：Web Service  

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  • Biotechnology Basic（CBT教材）

    片倉啓雄, 藤山和仁, 西川美香子, 福井希一

    2006

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    Work type：Educational material   Location：大阪大学  

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  • Biotechnology英会話（CBT教材）

    片倉啓雄, 藤山和人, 西川美香子, 福井希一

    2005

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    Work type：Educational material   Location：大阪大学  

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Awards

  • 第９回 JSEE AWARD

    2020.6   日本工学教育協会  

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    Country：Japan

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  • 第5回 日本e-Learning大賞 文部科学大臣賞

    2008.7   日本工業新聞社  

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    Country：Japan

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  • 日本生物工学会論文賞

    2002.10   日本生物工学会  

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    Country：Japan

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Research Projects

  • Control of quantity and quality of membrane vesicles produced by lactic acid bacteria

    Grant number：22K05396  2022.4 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

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  • High-density cultivation method that does not allow lactic acid bacteria to produce lactic acid

    Grant number：21K05372  2021.4 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）

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  • The Construction of Ethics Education Program to Foster Engineers Who Aspire to Promote the Well-being of the Public

    Grant number：18H01067  2018.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Fudano Jun

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    Grant amount：\16770000 （ Direct Cost: \12900000 、 Indirect Cost：\3870000 ）

    We proposed a new educational model named as "Positive Engineering Education" (hereafter PE2) which combines traditional academic and professional engineering education with "well-being" education based on the findings of well-being science. We also presented the components of the positive engineering education program, including specific learning and educational objectives and contents of foundation courses and the concept and implementation plan of "Well-being across the Curriculum."
    In addition, as educational materials to achieve the overall educational goal of PE2, we planned and produced video materials depicting a student's process of deepening his recognition of his own WB and how it relates to "good work" as an engineer.

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  • Regulation of lactic acid bacteria-derived membrane vesicle production with immunostimulatory activity and their functionality design

    Grant number：18K04857  2018.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    YAMASAKI Shino

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    Grant amount：\4420000 （ Direct Cost: \3400000 、 Indirect Cost：\1020000 ）

    Immunoglobulin A (IgA) plays an important role in defense mechanisms against pathogens on mucosal surfaces. Membrane vesicles (MVs) produced by a lactic acid bacterium, Latilactobacillus sakei enhanced IgA production in Peyer's patch cells isolated from the murine small intestine. To apply MVs as safe mucosal vaccine adjuvants, we analyzed the mechanisms of MV-mediated IgA production enhancement in Peyer's patch cells. It was excluded that MVs activated dendritic cells via Toll-like receptor 2 and induced the production of several factors involved in the class switch recombination of B cells and their differentiation into plasma cells. In addition, we found that culture factors such as culture period and temperature affected not only the amount of MVs produced by lactic acid bacteria but also the immunostimulatory effect, indicating the need to consider both the amount and the quality of MV.

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  • 免疫賦活作用をもたらす乳酸菌の表層に露出したリポテイコ酸部分構造の解析

    2017 - 2019

    日本学術振興会  科学研究費補助金，基盤研究C 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \3700 ）

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  • Engineering ethics education improvement by application of a standardized class questionnaire

    Grant number：26350200  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Okita Yuji, MARUYAMA Takeo, FUDANO Jun, KATAKURA Yoshio, ITO Yuko, AOYAGI Manabu

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    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

    The most prominent outcome of this study is to enable to provide the result of the class questionnaire quantitative data set which could be used to improve the quality of the engineering ethics education.
    The teaching materials developed from the questionnaire, the common usage of teaching tips and materials among the concerned lectures, the analytical results of the better placement of engineering ethics subjects in the curriculum are also the outcomes of this study.

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  • 乳酸を生産させない乳酸菌の培養法

    2014 - 2015

    日本学術振興会  科学研究費補助金，挑戦的萌芽研究 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \3900 ）

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  • 地域資源の高度利用を図るバイオリファイナリーの基盤形成とその実用化

    2013 - 2017

    文部科学省  私立大学戦略的研究基盤形成支援事業 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \172941 ）

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  • 文部科学省私立大学戦略的研究基盤形成支援事業「地域資源の高度利用を図るバイオリファイナリーの基盤形成とその実用化」

    2013 - 2017

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    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) 

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  • Engineering ethics education improvement by application of a standardized class questionnaire

    Grant number：23501028  2011 - 2013

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    OKITA Yuji, MATSUKI Junya, AOYAGI Manabu, SHIMIZU Kazuo, OBA Kyoko, KATAKURA Yoshio, SHIMOMURA Naoyuki, MARUYAMA Takeo

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    Grant amount：\5070000 （ Direct Cost: \3900000 、 Indirect Cost：\1170000 ）

    The goal of this research is to improve the teaching of engineering ethics in higher education by application of a standardized class questionnaire. The principal investigator developed a standardized questionnaire for an engineering ethics class by eliciting responses from a group of instructors of engineering ethics education.31 questions which were classified to five area were developed and applied to several engineering ethics classes of Japanese colleges and universities.The surveys were administered to several classes at the beginning and ending periods by a group of collaborative instructors so that they were able to assess changes of learners and thereby evaluate their educational quality. Changes and differences within and among classes were assessed. Many changes were found in attitudes, effort, and the level of understanding. By analyzing the results, the standardized class questionnaire has been shown to be useful for engineering ethics education improvement.

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  • Effects of intestinal bacterial adhesion to fibers on intestinal microflora

    Grant number：23656534  2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    KATAKURA Yoshio

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \4160 ）

    Surface proteins from Lactococcus lactis IL1403 cells purified by cellulose column were identified to be DnaK, GroEL, GAPDH and so on. The adsorption constants of the fluorescence labeled DnaK at pH 7.0 were estimated to be(2.7±0.9)×10^6、(2.5±0.5)×10^5、(3.7±2.2)×10^5、(8.7±3.2)×10^5 M^<-1> for cellulose, mucin, chitin and IL1403 cells, respectively. At pH 4.0, the adsorption constants for cellulose and mucin were estimated to be(5.7+-0.2) 10^6 and(4.4+-1.0) 10^6M^(-1), respectively. These results mean that lactic acid bacteria adhere to intestinal surface more tightly when they produce lactic acid.

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  • 地域分散型バイオエタノール生産のための省エネ低コスト固体連続並行複発酵に関する技術開発

    2010 - 2012

    環境省  地球温暖化対策技術開発事業 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \20800 ）

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  • Identifying Objectives, Teaching Methods, and Assessment Tools forEngineering Ethics Education: Toward the Development of a Model Syllabus

    Grant number：22500826  2010 - 2012

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    FUDANO Jun, ISHIHARA Koji, KATAKURA Yoshio, KIM Yonjyon, KOBAYASHI Yukito, TANAKA Hidekazu, TSUJII Hiroyuki, NATSUME Kenichi, MIKAMI Yoshiki, HOTTA Genji, SAEKI Noboru, YASUI Mitsukuni, FUJIKI Hiroyuki

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    Grant amount：\4810000 （ Direct Cost: \3700000 、 Indirect Cost：\1110000 ）

    In 2010 and 2011 the syllabi of 358 engineering-ethics-related courses taught in educational institutions across the country were collected and analyzed. Based on the results of the above syllabus surveys, 42 candidates for learning and educational objectives were extracted and categorized in the five domains. In 2012 they were evaluated by expert panels in the two-round Delphi survey using the Internet, and 22 objectives were defined and categorized into four domains.

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  • Delignification of cellulosic biomass by a bacterium

    Grant number：21360403  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KATAKURA Yoshio

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \13500 ）

    To establish biological delignification of biomass by Ureibacillus thermosphaericus, a rapid method evaluating the degree of deliginification using cellulose binding domain from Thermobifida fsuca Cel9B was developed. This bacterium was found to produce Mn-peroxidase and Cu-amine oxidase. It was possible to produce these enzymes by cultivating this bacterium in a synthetic medium containing seven amino acids and two vitamins.

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  • 乳酸菌が酵母を認識する機構の解明

    Grant number：21656217  2009 - 2010

    日本学術振興会  科学研究費補助金，挑戦的萌芽研究  挑戦的萌芽研究

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \3200 ）

    乳酸菌が自らが生産する乳酸のストレスを感じると、それを緩和してくれる酵母に接着し、より有利な環境を作り出すため、自身と酵母を包み込む多糖を生産する、という作業仮説を検証した。
    Lactobacillus casei ATCC334株とSaccharomyces cerevisiae IFO0216株を,脱脂粉乳培地を用いて30℃で共培養し、酵母と接着した乳酸菌の遺伝子発現をDNAマイクロアレイを用いて解析したところ、機能未知の膜タンパク質が多数誘導されており、細胞外多糖の生合成に関与すると推定されるタンパク質の発現が高まっていた。また、共培養によってATCC334株の細胞外多糖生産も高まっていおり、仮説が裏づけられた。
    Lactococcus lactis IL1403株のDnaKにHis-tagを付与して大腸菌で発現させた。精製したDnaKは、マンナン、酵母、乳酸菌に対して親和性をもち、0.1mg/ml以上の濃度では乳酸菌と酵母を凝集させた。また、ムチン、セルロース、キチン、デンプンに対して、それぞれ、5.1(+/-)0.9、2.7(+/-)0.9、1.1(+/-)0.3、0.2(+/-)0.06 x 10E6 1/Mの吸着定数を有していた。乳酸菌はいわゆる善玉細菌であり、腸の蠕動運動に逆らって定着するには、腸壁に接着する必要があり、これにはDnaKなどの乳酸菌表層のタンパク質が接着タンパク質としての役割を担っていると考えられる。同時に、DnaKがセルロース、キチン、デンプンなど食物繊維に対しても親和性をもつことは、腸内細菌の定着を議論する際には、食物繊維との相互作用も考慮しなければならないことを示している。

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  • セルロースエタノール高効率製造のための環境調和型統合プロセス開発

    2008 - 2010

    新エネルギー・産業技術総合開発機構  新エネルギー技術研究開発 バイオマスエネルギー等高効率転換技術開発（先導技術開発） 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \34500 ）

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  • 新エネルギー・産業技術総合開発機構(NEDO)，新エネルギー技術研究開発・バイオマスエネルギー等高効率転換技術開発，セルロースエタノール高効率製造のための環境調和型統合プロセス開発

    2008 - 2010

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    Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid) 

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  • Development of a tracking method for minor group in microbial community and its industrial application

    Grant number：19360374  2007 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    SHIOYA Suteaki, KATAKURA Yoshio, NINOMIYA Kazuaki, YAMAMOTO Shinjiro, HAYASHI Shuhei, FUJII Takao, NINOMIYA Kazuaki

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    Grant type：Competitive

    （ Direct Cost: \16140 ）

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  • 生物的無毒化処理によるセルロース系バイオマスの効率的利用

    2007

    科学技術振興機構  地域イノベーション創出総合支援事業「シーズ発掘試験」 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \2000 ）

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  • 微生物固体発酵による高効率なリグノセルロース完全利用システムの開発

    2007

    新エネルギー・産業技術総合開発機構  新エネルギー技術研究開発 バイオマスエネルギー等高効率転換技術開発（先導技術開発） 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \6000 ）

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  • 医薬品等の有効性・安全性を保証するための分析・解析技術の評価と標準化に関する研究

    2005 - 2006

    厚生労働省  厚生労働科学研究費補助金 創薬等ヒューマンサイエンス総合研究事業 

    林譲

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    Grant type：Competitive

    （ Direct Cost: \16100 ）

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  • 微生物固体発酵による高効率なリグノセルロース完全利用システムの開発

    2005 - 2006

    新エネルギー・産業技術総合開発機構  バイオマスエネルギー高効率転換技術開発 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \11000 ）

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  • 非分解性と信じられているプラスチックの分解酵素遺伝子のクローニング

    2004 - 2005

    日本学術振興会  科学研究費補助金萌芽研究 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \3500 ）

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  • 東南アジアにおける乳酸菌資源の学術調査及びデータベースの構築

    2004 - 2005

    日本学術振興会  科学研究費補助金基盤研究Ｂ海外学術調査 

    塩谷捨明

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    Grant type：Competitive

    （ Direct Cost: \11400 ）

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  • 微生物共生システムを利用した発酵生産法の開発

    2003 - 2004

    日本学術振興会  科学研究費補助金基盤研究Ｂ 

    塩谷捨明

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    Grant type：Competitive

    （ Direct Cost: \13300 ）

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  • Methodological Research of the Fuzzy Control in Insulin Production

    Grant number：12650752  2000 - 2002

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    KISHIMOTO Michimasa, OMASA Takeshi, KATAKURA Yoshio

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    Grant amount：\3100000 （ Direct Cost: \3100000 ）

    The objective of the research is the development of Fuzzy control system, which include the know-how of the superior fermentation technique, and is suitable for the production of Insulin by Saccharomyces cerevisiae. Insulin is important pharmaceuticals for diabetes, and produced mainly abroad, for example, Novo. Co. Ltd in Denmark has been producing Insulin using yeast However, we can easily imagine the severe technical problems in the production, because yeast produce alcohol, which seriously effect on the cell growth and the production. There fore, we need to perform many culture experiment in order to develop the fuzzy control algorithm and the on-line control system.
    In order to make sure the validity of the fuzzy control system, we tried to use the control system in the alcohol production by Saccharomyces cerevisiae. We succeeded to control the glucose feeding and to increase the growth rate and shorten culture time for the alcohol production. Our fuzzy control has a simple structure of fuzzy rules and membership functions compared with the normal fuzzy control, which has been developed in Japanese sake brewery company. Our approach applied to the immobilized cell system for alcohol production, and we can keep the glucose concentration at suitable level for the ethanol production successfully. In the fuzzy control, we have a severe problem which is the manual tuning of membership functions and manual adjustment of production rules, because we spend a lot of trouble for the procedures and they influence the efficiency of the fuzzy control serious ly. Then we tried to make the display system which shows the membership functions causing the fuzzy reasoning error. Taking into consideration of the suggestion from the display system, we modified the membership function. If the modification is small, the fuzzy control usually improved a little bit. But, if modified largely, the fuzzy control is damaged, because the many rule, which is not considered into the modification effect was effected by the modification. We also investigate the use of the proteome analysis technique for the fuzzy control development. Now, 2-D electrophoresis gel image is not stable but we succeed it to distinguish the culture state with the aid on ANN. Therefore we will be able to use the information from proteome analysis for the development of fuzzy control system.

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  • Analysis and Selection for gene-amplified cell based on FISH

    Grant number：11650817  1999 - 2001

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    OMASA Takeshi, KATAKURA Yoshio, KISHIMOTO Michimasa

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    Grant amount：\3400000 （ Direct Cost: \3400000 ）

    In order to establish an easy and quick construction method for obtaining a stable and highly productive gene-amplified recombinant Chinese Hamster Ovary (CHO) cell line, various kinds of stepwise methotrexate (MTX) selection were carried out. The specific growth and production rates of the cell were compared with each other, and the distribution of the amplified gene location was determined using fluorescence in situ hybridization (FISH). The specific growth and production rates of the cell pool reached the highest levels under the selection condition in which the stepwise increase in the MTX concentration was most gradual; about 82 % of amplified genes were observed near the telomeric region. To clarify the behavior of gene-amplified cell pools during a stepwise increase of MTX concentration, we investigated isolated gene-amplified clones derived from gene amplified cell pools. As a result, telomere-type clones, in which the amplified gene was located near the telomeric region, were found to be more stable and productive the other types of clones. Telomere-type clones had over 100 copies of amplified genes in the chromosomal DNA In contrast a large number of other types of clones had less than 10 copies of amplified genes. During long-term cultivation in the absence of MTX, in other types of clones, amplified genes rapidly decreased in the chromosomal DNA.
    Using a fluorescein isothiocyanatelabeled methotrexate (F-MTX) reagent with flow cytomery, we were easily able to distinguish between highly productive cells and the other types of cells. The level of fluorescence differed according to the difference in resistance to MTX. Based on this new finding, highly productive gene-amplified cells could be isolated from heterogeneous gene amplified cell pools more easily than by the method of limiting dilution assay. The limiting-dilution method requires several months to obtain highly productive gene amplified cells, while our flowcytometry-based method of selection requires only a few weeks.

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  • キャプチャリングカセット法によるファージディスプレイライブラリーの速度論的選抜

    1998 - 1999

    日本学術振興会  科学研究費補助金基盤研究Ｂ 

    福崎栄一郎

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    Grant type：Competitive

    （ Direct Cost: \13500 ）

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  • 塩橋の成立条件のファージディスプレイを利用した統計的解析

    1997

    日本学術振興会  科学研究費補助金重点領域研究(2) 

    片倉啓雄

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    Authorship：Principal investigator  Grant type：Competitive

    （ Direct Cost: \1900 ）

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  • Construction of hybrid liver support system using gene amplified recombinant mammalian cells

    Grant number：07558125  1995 - 1997

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    SUGA Ken-ichi, OMASA Takeshi, KATAKURA Yoshio, KISHIMOTO Michimasa

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    Grant amount：\12600000 （ Direct Cost: \12600000 ）

    In order to establish the cell line which was suitable for hybrid artificial liver support system, an ammonia metabolizing activity was given to Chinese Hamster Ovary (CHO) and HepG2 cell line using genetic engineering techniques. CHO-K1 and HepG2 cells were transformed with pSV2-GS vector which contains glutamine synthetase gene connected with SV40 or CMV promoter. The recombinant cell line was selected under the various concentrations of glutamine synthetase inhibitor, methionine sulfoximine (MSX). The obtained MSX tolerable cell line had the high glutamine synthetase activity and could remove the ammonium from medium. The kinetic parameters of this cell line were determined under 0,1, or 2mM ammonia concentration. The specific ammonium consumption rates of established CHO and HepG2 cell line during growth phase were about one fourth and one seventh of that of primary hepatocyte respectively.

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  • オリゴペプチドリガンドを用いたアフィニティ分離システム

    Grant number：07855092  1995

    日本学術振興会  科学研究費助成事業  奨励研究(A)

    片倉 啓雄

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    Grant amount：\900000 （ Direct Cost: \900000 ）

    【方法】ファージベクターfUSE5を用いて、コート蛋白質g3のN末端に、2つのシステイン残基に挟まれた6残基のランダムペプチドが発現するようにランダムペプチドライブラリーを構築した。モデル蛋白質として牛膵臓リボヌクレアーゼ(RNaseA)を用いてこれに対して親和性を持つペプチドを提示するファージクローンを、ライブラリーから固層に固定したRNaseAによって選択した。この際、得られるペプチドとRNaseAとの間の静電的な結合が強調されるように、RNaseAの等電点(9.6)から離れたpH6.0で、かつイオン強度の低い10mMリン酸緩衝液を用いてアフィニティ選択を行った。
    【結果】RNaseAに対して親和性を持つペプチドを提示したファージクローンが得られ、これらのファージのRNaseAに対する親和性はイオン強度を上昇させると顕著に低下した。現在塩基配列からこのクローンの提示するペプチドの配列を解析しているが、これまでに得られた結果から、(1)目的蛋白質に対する親和性を有するオリゴペプチドリガンドが取得でき、(2)得られたリガンドの結合はイオン強度依存性が顕著であることが明らかとなった。
    【結論】以上のことから、本研究に用いた手法によって、NaClの添加などによる温和な条件で目的蛋白質を溶出回収することができる理想的なペプチドリガンドが得られると考えられる。

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Teaching Experience

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Social Activities

  • 安全・技術者倫理関係の講演（トライボロジー学会大会(2015年)、日本乳酸菌学会泊り込み研究会(2015年)、日本電気学会全国大会シンポジウム(2014年)、電気学会教育フロンティア研究会(2014)、日本工学教育協会(2010, 2009, 2008年)、有明工業高等専門学校(2010年)、電気学会教育フロンティア研究会(2009年)、宮崎大学工学部(2009年)、日本鉄鋼協会大会(2009年)、工業教育研究講演会(2008年)、青山学院大学理工学部(2007年)、日本機械学会大会(2007年)、私立大学環境保全協議会第22回夏期研修研究会(2007年)、東京大学工学研究科(2007年)、愛媛大学農学部(2007年)、室蘭工業大学(2006年)）

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Devising educational methods

  • ・微生物学2の授業では、以下の施行を行った。 (1)　15回の授業それぞれの内容を問う記述式の問題を3つ程度、初回の授業で配布した。 (2)　学生にはそれぞれの問題について予習をさせ、授業後にミニッツペーパーで解答を提出させた。 (3)　添削して翌週の授業の最初に返却して講評を行った。 その結果、期末試験では平均点が前年に比べて3.5点向上した。
    ・フレッシュマンゼミナールでは、要点を聞き取りメモを取る方法を教授し、ミニッツペーパーに書かせた授業の要点をその場で添削指導している。また、覚えることと理解することの違いを説明した上、学ぶ目的を各自に設定させている。
    ・学部4年生の安全教育において、研究室間の相互巡視を行わせ、良い点を翌週の授業で報告させる。さらに、それを踏まえて自研究室の問題点を改善させ、報告させる。 ・大学院生に自身の研究テーマの意義、課題、解決のための戦略をまとめたプレゼンをさせ、研究をデザインする力を養う。

Teaching materials

  • 1． 片倉啓雄 他3名「Biotechnology Basic」、大阪大学（2006） 工学系の学部学生の専門英語力向上のため、再生医療などの最新のトピックスを基本学術用語とともに解説したnative speakerの音声と教材の画像が利用できるCBT（computer based training）教材。
    2． 片倉啓雄 他4名「Biotechnology 英会話」、大阪大学（2006） 工学系の学部学生の専門英語力向上のため、専門的な内容の会話、プレゼンテーションのための基本構文などをまとめたnative speakerの音声と教材の画像が利用できるCBT（computer based training）教材。
    3． 片倉啓雄・堀田源治「安全倫理」、培風館（2008）. 社会の安全・安心を実現するための現実的な考え方と具体的なテクニック、日頃からそれを実践するためのポイントを、ケーススタディーをまじえて平易に解説した。
    4． 片倉啓雄・山本仁「バイオ系実験安全オリエンテーションDVD付き」、東京化学同人（2009） 生化学系実験の安全のため、基本操作と事故の再現シーンのDVDを作成し、解説と演習問題をまとめた。
    5． 片倉啓雄「遺伝子工学」（基礎生物学テキストシリーズ）近藤昭彦、芝崎誠司編、化学同人、第5章「遺伝子クローニング」pp68-82　(2012)
    6． 片倉啓雄，大政健史，長沼孝文，小野比佐好 編著「有用微生物培養のイロハ」pp. 1-5, 18-81, 93-98, 129-137, 153-185, 195-249, 280-288, 323-324（337頁中183頁執筆）, エヌ・ティ・エス (2014)
    7． 片倉啓雄「実践・化学英語リスニング生化学編」 Part 2, Chapter 4～6，福井希一・福住俊一・上田サーソン編，化学同人 (2014).
    8． 片倉啓雄，「大学生のための「安全・安心」の基礎講座」第7講「個人にできること（Ⅰ）倫理・不正防止」日本国立大学協会編 (2015). 9. 片倉啓雄，大政健史，長沼孝文，小野比佐好 編著「有用微生物培養のイロハ」改訂第二版, pp. 1-5, 18-87, 90, 101-106, 131, 139-167, 183-216, 222, 225-256, 265-280, 310-318, 353-354（367頁中205頁執筆, 初版に33頁加筆）, エヌ・ティ・エス (2018)
    9. 片倉啓雄,「バイオ実験を安全に行うために」第3章, pp45-77（単著）, 第4章, pp79-107（共著）, 化学同人 (2018)

Teaching method presentations

  • 1. 「「世界に通用するProfessional Engineerを育てるための安全教育」報告書」，三恵社（2006）
    2. 「学生自身に考えさせるボトムアップ的な安全・倫理教育を目指して」、第1回安心と安全のための教育シンポジウム「教え子の安全・安心・健康への感性を高めるには」要旨集p15-20、2006年1月31日、大阪大学
    3. 「研究室での安全への取り組み－大阪大学での好事例を中心に－」、第2回安心と安全のための教育シンポジウム「安全・安心な教育研究環境を実現するには」要旨集p2-7、2007年1月30日、大阪大学
    4. 「安全にはトップダウンとボトムアップの双方が必要－学生に能動的に安全活動に参加させるには－」私立大学環境保全協議会第22回夏期研修研究会、2007年8月7日、同志社大学
    5. 「工学倫理教育とバイオテクノロジー」、平成20年度日本機械学会大会、2007年9月9-12日、関西大学
    6． 「安全を保証される立場から保証する立場へ」、日本工学教育協会 第8回ワークショップ「技術者倫理」、2008年2月2日、日本大学理工学部
    7． 「技術者倫理教育と安全教育との融合－技術者が知るべき実践的安全倫理－」平成20年度工学・工業教育研究講演会講演論文集、p290-291、2008年8月1-3日、神戸大学
    8． 「安全倫理－気づかせ、考えさせ、実践させる－」日本工学教育協会 第9回ワークショップ「技術者倫理」、2009年2月21日、日本大学理工学部
    9． 「安全倫理－社会の安全・安心のための基本をどのように教えるか－」日本鉄鋼協会 社会鉄鋼工学部会安全・快適なシステム構築研究フォーラム 第13回公開セミナー、2008年11月20日、日本鉄鋼協会
    10． 「大学での安全教育－学生自身に気づかせ、考えさせ、実践させる－」日本鉄鋼協会第157回春季講演大会、2009年3月30日、東京工業大学
    11． 「安全を保証される立場から保証する立場へ－安全教育と技術者倫理教育の融合－」第3回専門職・技術者倫理ワークショップ、2009年11月25日、宮崎大学
    12． 「技術者倫理教育の目的は何か－社会の安全・安心との関連を中心に考える－」電気学会教育フロンティア研究会、2009年12月11,12日、福井大学
    13． 「倫理的問題が生じる背景と今時の学生の気質」日本工学教育協会 第10回ワークショップ「技術者倫理」、2010年2月26-27日、金沢大学東京虎ノ門キャンパス
    14． 「社会の安全・安心と技術者倫理教育」日本工学教育協会 第11回ワークショップ技術者倫理、2011年2月26日、金沢大学東京虎ノ門キャンパス
    15． 「実験系学部の安全教育 …無知の罪を知り、既知の危険を学び、未知の危険に対処する人材を育てるために」日本鉄鋼協会第163回春季講演大会, 新時代の安全マネジメントフォーラム（招待），2012年，3/30，横浜国立大学
    16． 「キャリアデザインと技術者倫理」電気学会教育フロンティア研究会, 研究会資料 FIE 2013(25-38), 7-9, 2013年, 12/6, 関西大学
    17． 「いかにして技術者の責務とやりがいに気付かせるか」日本電気学会全国大会シンポジウム, 1-1H-2, 2014年3月19日, 愛媛大学
    18． 「技術者倫理教育の目的は何か」日本トライボロジー学会2015年会, 2015年5月27日, 姫路商工会議所
    19. 片倉啓雄“技術者・研究者にとってののwell-beingとは”日本乳酸菌学会泊り込み研究会（招待）, 2015年5月15日, サンライズ淡路
    20. 片倉啓雄“技術者倫理への動機づけを高める指導法” 日本工学教育協会 第16回ワークショップ「技術者倫理」（招待），2015年12月11-12日，東京工業大学大岡山キャンパス．
    21. 片倉啓雄“研究倫理をどのように教えるか－技術者倫理教育の視点から－”AMED RIOネットワーク意見聴取会（招待）, 2016年12月9日, 新大阪・CICI研修センター.
    22. 片倉啓雄“研究倫理をどのように教えるか－技術者倫理教育の視点から－”AMED RIOネットワーク意見聴取会（招待）, 2017年1月20日, 東京・大手町.
    23. 片倉啓雄“受講者の価値観に刺激を与える授業方法”日本工学教育協会 第15回ワークショップ「技術者倫理」（招待），2017年2月3-4日, 東京工業大学.
    24. 片倉啓雄“技術者・研究者が知るべき倫理－あなたのWell-beingのために－”信州大学研究者倫理特別講義, 2017年6月20日, 信州大学
    25. 片倉啓雄“技術者・研究者が知るべき倫理－あなたのWell-beingのために－”有明工業高等専門学校, 2017年10月18日, 有明高等専門学校
    26. 片倉啓雄“科学に携わる者の倫理―技術者倫理の視点から―”第31回日本耳鼻咽喉科学会専門医講習会（招待）, 2017年11月11日, 12日, 神戸ポートピアホテル
    27. 片倉啓雄“科学に携わる者の倫理―技術者倫理の視点から―”日本医療研究開発機構, 研究公正シンポジウム「考え、気づかせる」研究倫理教育（招待）, 2017年11月29日, 東京・北とぴあ
    28. 片倉啓雄“志向倫理教育の実践例”第3回ワークショップ「研究倫理」（招待）, 2018年2月17日, 金沢大学東京虎ノ門キャンパス．
    29. 片倉啓雄“科学に携わる者のための倫理”千葉市医師会医療安全講習会（招待）, 2018年6月8日, 千葉市総合保健医療センター
    30. 片倉啓雄“科学に携わる者のための倫理”川崎医療福祉大学 平成30年度コンプライアンス研修（招待）, 2018年5月15日
    31. 片倉啓雄“科学に携わる者のための倫理　～技術者倫理教育の視点から～ ”京都府立医科大学倫理研修会（招待）, 2018年7月18日
    32. 片倉啓雄“志向倫理教育の実践例―ルールを提案できる人材の育成―”平成30年度工学教育研究講演会講演論文集，p，2018年8月29-31日，名古屋工業大学．
    33. 片倉啓雄“科学に携わる者のための倫理　～技術者倫理教育の視点から～ ”横浜市立大学コンプライアンスセミナー（招待）, 2018年9月27日

Special notes on other educational activities

  • 2009年～ 技術者倫理教育に関するワークシップ（日本工学教育協会）オーガナイザー
    2008年7月第5回 日本e-Learning大賞 文部科学大臣賞（大阪大学現代GP事務局・専門英語e-Learningプログラム開発グループとして）
    2006年、2007年に「安心と安全のための教育シンポジウム」を主催。